Extensive precursor-product relationship between gangliosides formed from exogenous glucosylceramide in rat liver

Marco Trinchera, Carlo Vallenzasca, Riccardo Ghidoni, Guido Tettamanti

Research output: Contribution to journalArticle

Abstract

Glucosylceramide, radiolabelled on the glucose residue, was administered to rats and the radioactive gangliosides formed at different time points were chemically characterized. They were identified as GM3, GM1, GD1a and GD1b, each one carrying only radioactive glucose. The time course of each individual ganglioside showed that the simpler gangliosides were formed earlier but were consumed earlier than the more complex ones, resulting in radioactivity patterns that were different at each time point. Only 30 h after injection did it resemble that of endogenous rat liver gangliosides. These results indicate that an extensive precursor-product relationship actually exists in the course of ganglioside biosynthesis.

Original languageEnglish
Pages (from-to)23-26
Number of pages4
JournalFEBS Letters
Volume260
Issue number1
DOIs
Publication statusPublished - Jan 15 1990

Fingerprint

Glucosylceramides
Gangliosides
Liver
Rats
Glucose
Biosynthesis
Radioactivity
Injections

Keywords

  • Ganglioside
  • Glucosylceramide
  • Glycosylation
  • Golgi apparatus

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Extensive precursor-product relationship between gangliosides formed from exogenous glucosylceramide in rat liver. / Trinchera, Marco; Vallenzasca, Carlo; Ghidoni, Riccardo; Tettamanti, Guido.

In: FEBS Letters, Vol. 260, No. 1, 15.01.1990, p. 23-26.

Research output: Contribution to journalArticle

Trinchera, Marco ; Vallenzasca, Carlo ; Ghidoni, Riccardo ; Tettamanti, Guido. / Extensive precursor-product relationship between gangliosides formed from exogenous glucosylceramide in rat liver. In: FEBS Letters. 1990 ; Vol. 260, No. 1. pp. 23-26.
@article{7264819b4397414783416d25dce394c4,
title = "Extensive precursor-product relationship between gangliosides formed from exogenous glucosylceramide in rat liver",
abstract = "Glucosylceramide, radiolabelled on the glucose residue, was administered to rats and the radioactive gangliosides formed at different time points were chemically characterized. They were identified as GM3, GM1, GD1a and GD1b, each one carrying only radioactive glucose. The time course of each individual ganglioside showed that the simpler gangliosides were formed earlier but were consumed earlier than the more complex ones, resulting in radioactivity patterns that were different at each time point. Only 30 h after injection did it resemble that of endogenous rat liver gangliosides. These results indicate that an extensive precursor-product relationship actually exists in the course of ganglioside biosynthesis.",
keywords = "Ganglioside, Glucosylceramide, Glycosylation, Golgi apparatus",
author = "Marco Trinchera and Carlo Vallenzasca and Riccardo Ghidoni and Guido Tettamanti",
year = "1990",
month = "1",
day = "15",
doi = "10.1016/0014-5793(90)80056-O",
language = "English",
volume = "260",
pages = "23--26",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Extensive precursor-product relationship between gangliosides formed from exogenous glucosylceramide in rat liver

AU - Trinchera, Marco

AU - Vallenzasca, Carlo

AU - Ghidoni, Riccardo

AU - Tettamanti, Guido

PY - 1990/1/15

Y1 - 1990/1/15

N2 - Glucosylceramide, radiolabelled on the glucose residue, was administered to rats and the radioactive gangliosides formed at different time points were chemically characterized. They were identified as GM3, GM1, GD1a and GD1b, each one carrying only radioactive glucose. The time course of each individual ganglioside showed that the simpler gangliosides were formed earlier but were consumed earlier than the more complex ones, resulting in radioactivity patterns that were different at each time point. Only 30 h after injection did it resemble that of endogenous rat liver gangliosides. These results indicate that an extensive precursor-product relationship actually exists in the course of ganglioside biosynthesis.

AB - Glucosylceramide, radiolabelled on the glucose residue, was administered to rats and the radioactive gangliosides formed at different time points were chemically characterized. They were identified as GM3, GM1, GD1a and GD1b, each one carrying only radioactive glucose. The time course of each individual ganglioside showed that the simpler gangliosides were formed earlier but were consumed earlier than the more complex ones, resulting in radioactivity patterns that were different at each time point. Only 30 h after injection did it resemble that of endogenous rat liver gangliosides. These results indicate that an extensive precursor-product relationship actually exists in the course of ganglioside biosynthesis.

KW - Ganglioside

KW - Glucosylceramide

KW - Glycosylation

KW - Golgi apparatus

UR - http://www.scopus.com/inward/record.url?scp=0025177046&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025177046&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(90)80056-O

DO - 10.1016/0014-5793(90)80056-O

M3 - Article

C2 - 2298294

AN - SCOPUS:0025177046

VL - 260

SP - 23

EP - 26

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -