Abstract
Glucosylceramide, radiolabelled on the glucose residue, was administered to rats and the radioactive gangliosides formed at different time points were chemically characterized. They were identified as GM3, GM1, GD1a and GD1b, each one carrying only radioactive glucose. The time course of each individual ganglioside showed that the simpler gangliosides were formed earlier but were consumed earlier than the more complex ones, resulting in radioactivity patterns that were different at each time point. Only 30 h after injection did it resemble that of endogenous rat liver gangliosides. These results indicate that an extensive precursor-product relationship actually exists in the course of ganglioside biosynthesis.
| Original language | English |
|---|---|
| Pages (from-to) | 23-26 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 260 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 15 1990 |
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Keywords
- Ganglioside
- Glucosylceramide
- Glycosylation
- Golgi apparatus
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Extensive precursor-product relationship between gangliosides formed from exogenous glucosylceramide in rat liver. / Trinchera, Marco; Vallenzasca, Carlo; Ghidoni, Riccardo; Tettamanti, Guido.
In: FEBS Letters, Vol. 260, No. 1, 15.01.1990, p. 23-26.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Extensive precursor-product relationship between gangliosides formed from exogenous glucosylceramide in rat liver
AU - Trinchera, Marco
AU - Vallenzasca, Carlo
AU - Ghidoni, Riccardo
AU - Tettamanti, Guido
PY - 1990/1/15
Y1 - 1990/1/15
N2 - Glucosylceramide, radiolabelled on the glucose residue, was administered to rats and the radioactive gangliosides formed at different time points were chemically characterized. They were identified as GM3, GM1, GD1a and GD1b, each one carrying only radioactive glucose. The time course of each individual ganglioside showed that the simpler gangliosides were formed earlier but were consumed earlier than the more complex ones, resulting in radioactivity patterns that were different at each time point. Only 30 h after injection did it resemble that of endogenous rat liver gangliosides. These results indicate that an extensive precursor-product relationship actually exists in the course of ganglioside biosynthesis.
AB - Glucosylceramide, radiolabelled on the glucose residue, was administered to rats and the radioactive gangliosides formed at different time points were chemically characterized. They were identified as GM3, GM1, GD1a and GD1b, each one carrying only radioactive glucose. The time course of each individual ganglioside showed that the simpler gangliosides were formed earlier but were consumed earlier than the more complex ones, resulting in radioactivity patterns that were different at each time point. Only 30 h after injection did it resemble that of endogenous rat liver gangliosides. These results indicate that an extensive precursor-product relationship actually exists in the course of ganglioside biosynthesis.
KW - Ganglioside
KW - Glucosylceramide
KW - Glycosylation
KW - Golgi apparatus
UR - http://www.scopus.com/inward/record.url?scp=0025177046&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025177046&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(90)80056-O
DO - 10.1016/0014-5793(90)80056-O
M3 - Article
C2 - 2298294
AN - SCOPUS:0025177046
VL - 260
SP - 23
EP - 26
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -