Extracellular acidic pH modulates oxygen-dependent cytotoxic responses mediated by polymorphonuclear leucocytes and monocytes

J. R. Geffner, A. S. Trevani, F. Minnucci, M. S. Palermo, N. Maugeri, M. A. Isturiz

Research output: Contribution to journalArticle

Abstract

In the present study, we compared the ability of human neutrophils and monocytes to display oxygen-dependent cytotoxic responses at pH 7.4 and 6.2. Our results show that cytotoxicity induced by immune complexes (IC), zymosan, N-formyl-methionyl-leucyl-phenylalanine (FMLP) and concanavalin A (ConA) were markedly increased when they were carried out at pH 6.2 instead of pH 7.4. Cytotoxicity induced by phorbol myristate acetate (PMA), on the contrary, was significantly decreased at pH 6.2. It is noteworthy that cytotoxic responses induced by IC, zymosan and Con A were also increased when, 2 h after effector cell stimulation at pH 6.2, cytotoxicity was measured at pH 7.4. Finally, when we examined possible mechanisms involved in the augmentation of cytotoxicity, we observed that the oxidative response of IC-stimulated neutrophils, measured as chemiluminescence emission, was not increased at pH 6.2, on the contrary, it was significantly decreased. The relevance of these results is discussed.

Original languageEnglish
Pages (from-to)164-169
Number of pages6
JournalClinical and Experimental Immunology
Volume91
Issue number1
Publication statusPublished - 1993

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Keywords

  • cytotoxicity
  • monocyte
  • neutrophil
  • pH

ASJC Scopus subject areas

  • Immunology

Cite this

Geffner, J. R., Trevani, A. S., Minnucci, F., Palermo, M. S., Maugeri, N., & Isturiz, M. A. (1993). Extracellular acidic pH modulates oxygen-dependent cytotoxic responses mediated by polymorphonuclear leucocytes and monocytes. Clinical and Experimental Immunology, 91(1), 164-169.