Extracellular ATP induces the rapid release of HIV-1 from virus containing compartments of human macrophages

Francesca Graziano, Marion Desdouits, Livia Garzetti, Paola Podini, Massimo Alfano, Anna Rubartelli, Roberto Furlan, Philippe Benaroch, Guido Poli

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

HIV type 1 (HIV-1) infects CD4+ T lymphocytes and tissue macrophages. Infected macrophages differ from T cells in terms of decreased to absent cytopathicity and for active accumulation of new progeny HIV-1 virions in virus-containing compartments (VCC). For these reasons, infected macrophages are believed to act as "Trojan horses" carrying infectious particles to be released on cell necrosis or functional stimulation. Here we explored the hypothesis that extracellular ATP (eATP) could represent a microenvironmental signal potentially affecting virion release from VCC of infected macrophages. Indeed, eATP triggered the rapid release of infectious HIV-1 from primary human monocyte-derived macrophages (MDM) acutely infected with the CCR5-dependent HIV-1 strain. A similar phenomenon was observed in chronically infected promonocytic U1 cells differentiated to macrophage-like cells (D-U1) by costimulation with phorbol esters and urokinase-type plasminogen activator. Worthy of note, eATP did not cause necrotic, apoptotic, or pyroptotic cell death, and its effect on HIV-1 release was suppressed by Imipramine (an antidepressant agent known to inhibit microvesicle formation by interfering with membrane-associated acid sphingomyelinase). Virion release was not triggered by oxidized ATP, whereas the effect of eATP was inhibited by a specific inhibitor of the P2X7 receptor (P2X7R). Thus, eATP triggered the discharge of virions actively accumulating in VCC of infected macrophages via interaction with the P2X7R in the absence of significant cytopathicity. These findings suggest that the microvesicle pathway and P2X7R could represent exploitable targets for interfering with the VCC-associated reservoir of infectious HIV-1 virions in tissue macrophages.

Original languageEnglish
Pages (from-to)E3265-E3273
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number25
DOIs
Publication statusPublished - Jun 23 2015

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HIV-1
Adenosine Triphosphate
Macrophages
Viruses
Virion
Purinergic P2X7 Receptors
Virus Release
T-Lymphocytes
Sphingomyelin Phosphodiesterase
Imipramine
Urokinase-Type Plasminogen Activator
Phorbol Esters
Antidepressive Agents
Necrosis
Acids
Membranes

Keywords

  • ATP
  • HIV
  • Macrophage
  • P2X7
  • Virion-containing compartment

ASJC Scopus subject areas

  • General

Cite this

Extracellular ATP induces the rapid release of HIV-1 from virus containing compartments of human macrophages. / Graziano, Francesca; Desdouits, Marion; Garzetti, Livia; Podini, Paola; Alfano, Massimo; Rubartelli, Anna; Furlan, Roberto; Benaroch, Philippe; Poli, Guido.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, No. 25, 23.06.2015, p. E3265-E3273.

Research output: Contribution to journalArticle

Graziano, Francesca ; Desdouits, Marion ; Garzetti, Livia ; Podini, Paola ; Alfano, Massimo ; Rubartelli, Anna ; Furlan, Roberto ; Benaroch, Philippe ; Poli, Guido. / Extracellular ATP induces the rapid release of HIV-1 from virus containing compartments of human macrophages. In: Proceedings of the National Academy of Sciences of the United States of America. 2015 ; Vol. 112, No. 25. pp. E3265-E3273.
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