Extracellular copper ions regulate cellular prion protein (PrPC) expression and metabolism in neuronal cells

Mattia Toni, Maria Lina Massimino, Cristiana Griffoni, Benedetto Salvato, Vittorio Tomasi, Enzo Spisni

Research output: Contribution to journalArticlepeer-review

Abstract

The physiological functions of cellular prion protein (PrPC) remain unclear. It has been demonstrated that PrPC is a copper binding protein and proposed that its functions could be strictly linked to copper metabolism and neuroprotection. The aim of this study was to clarify how extracellular copper modifies PrPC expression and metabolism in cultured neurones. We reported here that copper delivered at physiological concentrations significantly decreases PrPC mRNA expression in GN11 neurones. Moreover, copper increases the release of PrPC into the culture medium. These results indicate that extracellular copper strongly affects the amount of cellular PrP and might represent an interesting strategy to decrease the expression of PrPC in neurones and its conversion in the pathological isoform PrPSc.

Original languageEnglish
Pages (from-to)741-744
Number of pages4
JournalFEBS Letters
Volume579
Issue number3
DOIs
Publication statusPublished - Jan 31 2005

Keywords

  • Cellular prion protein expression
  • Cellular prion protein physiology
  • Copper metabolism
  • GN11 cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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