Abstract
The physiological functions of cellular prion protein (PrPC) remain unclear. It has been demonstrated that PrPC is a copper binding protein and proposed that its functions could be strictly linked to copper metabolism and neuroprotection. The aim of this study was to clarify how extracellular copper modifies PrPC expression and metabolism in cultured neurones. We reported here that copper delivered at physiological concentrations significantly decreases PrPC mRNA expression in GN11 neurones. Moreover, copper increases the release of PrPC into the culture medium. These results indicate that extracellular copper strongly affects the amount of cellular PrP and might represent an interesting strategy to decrease the expression of PrPC in neurones and its conversion in the pathological isoform PrPSc.
| Original language | English |
|---|---|
| Pages (from-to) | 741-744 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 579 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jan 31 2005 |
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Keywords
- Cellular prion protein expression
- Cellular prion protein physiology
- Copper metabolism
- GN11 cell
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Extracellular copper ions regulate cellular prion protein (PrPC) expression and metabolism in neuronal cells. / Toni, Mattia; Massimino, Maria Lina; Griffoni, Cristiana; Salvato, Benedetto; Tomasi, Vittorio; Spisni, Enzo.
In: FEBS Letters, Vol. 579, No. 3, 31.01.2005, p. 741-744.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Extracellular copper ions regulate cellular prion protein (PrPC) expression and metabolism in neuronal cells
AU - Toni, Mattia
AU - Massimino, Maria Lina
AU - Griffoni, Cristiana
AU - Salvato, Benedetto
AU - Tomasi, Vittorio
AU - Spisni, Enzo
PY - 2005/1/31
Y1 - 2005/1/31
N2 - The physiological functions of cellular prion protein (PrPC) remain unclear. It has been demonstrated that PrPC is a copper binding protein and proposed that its functions could be strictly linked to copper metabolism and neuroprotection. The aim of this study was to clarify how extracellular copper modifies PrPC expression and metabolism in cultured neurones. We reported here that copper delivered at physiological concentrations significantly decreases PrPC mRNA expression in GN11 neurones. Moreover, copper increases the release of PrPC into the culture medium. These results indicate that extracellular copper strongly affects the amount of cellular PrP and might represent an interesting strategy to decrease the expression of PrPC in neurones and its conversion in the pathological isoform PrPSc.
AB - The physiological functions of cellular prion protein (PrPC) remain unclear. It has been demonstrated that PrPC is a copper binding protein and proposed that its functions could be strictly linked to copper metabolism and neuroprotection. The aim of this study was to clarify how extracellular copper modifies PrPC expression and metabolism in cultured neurones. We reported here that copper delivered at physiological concentrations significantly decreases PrPC mRNA expression in GN11 neurones. Moreover, copper increases the release of PrPC into the culture medium. These results indicate that extracellular copper strongly affects the amount of cellular PrP and might represent an interesting strategy to decrease the expression of PrPC in neurones and its conversion in the pathological isoform PrPSc.
KW - Cellular prion protein expression
KW - Cellular prion protein physiology
KW - Copper metabolism
KW - GN11 cell
UR - http://www.scopus.com/inward/record.url?scp=12744263653&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12744263653&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2004.12.053
DO - 10.1016/j.febslet.2004.12.053
M3 - Article
VL - 579
SP - 741
EP - 744
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -