Extracellular copper ions regulate cellular prion protein (PrPC) expression and metabolism in neuronal cells

Mattia Toni; Maria Lina Massimino; Cristiana Griffoni; Benedetto Salvato; Vittorio Tomasi; Enzo Spisni

doi:10.1016/j.febslet.2004.12.053

Extracellular copper ions regulate cellular prion protein (PrPC) expression and metabolism in neuronal cells

Mattia Toni, Maria Lina Massimino, Cristiana Griffoni, Benedetto Salvato, Vittorio Tomasi, Enzo Spisni

Istituti Ortopedici Rizzoli

Research output: Contribution to journal › Article › peer-review

Abstract

The physiological functions of cellular prion protein (PrP^C) remain unclear. It has been demonstrated that PrP^C is a copper binding protein and proposed that its functions could be strictly linked to copper metabolism and neuroprotection. The aim of this study was to clarify how extracellular copper modifies PrP^C expression and metabolism in cultured neurones. We reported here that copper delivered at physiological concentrations significantly decreases PrP^C mRNA expression in GN11 neurones. Moreover, copper increases the release of PrP^C into the culture medium. These results indicate that extracellular copper strongly affects the amount of cellular PrP and might represent an interesting strategy to decrease the expression of PrP^C in neurones and its conversion in the pathological isoform PrP^Sc.

Original language	English
Pages (from-to)	741-744
Number of pages	4
Journal	FEBS Letters
Volume	579
Issue number	3
DOIs	https://doi.org/10.1016/j.febslet.2004.12.053
Publication status	Published - Jan 31 2005

Keywords

Cellular prion protein expression
Cellular prion protein physiology
Copper metabolism
GN11 cell

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/j.febslet.2004.12.053

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title = "Extracellular copper ions regulate cellular prion protein (PrPC) expression and metabolism in neuronal cells",

abstract = "The physiological functions of cellular prion protein (PrPC) remain unclear. It has been demonstrated that PrPC is a copper binding protein and proposed that its functions could be strictly linked to copper metabolism and neuroprotection. The aim of this study was to clarify how extracellular copper modifies PrPC expression and metabolism in cultured neurones. We reported here that copper delivered at physiological concentrations significantly decreases PrPC mRNA expression in GN11 neurones. Moreover, copper increases the release of PrPC into the culture medium. These results indicate that extracellular copper strongly affects the amount of cellular PrP and might represent an interesting strategy to decrease the expression of PrPC in neurones and its conversion in the pathological isoform PrPSc.",

keywords = "Cellular prion protein expression, Cellular prion protein physiology, Copper metabolism, GN11 cell",

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T1 - Extracellular copper ions regulate cellular prion protein (PrPC) expression and metabolism in neuronal cells

AU - Toni, Mattia

AU - Massimino, Maria Lina

AU - Griffoni, Cristiana

AU - Salvato, Benedetto

AU - Tomasi, Vittorio

AU - Spisni, Enzo

PY - 2005/1/31

Y1 - 2005/1/31

N2 - The physiological functions of cellular prion protein (PrPC) remain unclear. It has been demonstrated that PrPC is a copper binding protein and proposed that its functions could be strictly linked to copper metabolism and neuroprotection. The aim of this study was to clarify how extracellular copper modifies PrPC expression and metabolism in cultured neurones. We reported here that copper delivered at physiological concentrations significantly decreases PrPC mRNA expression in GN11 neurones. Moreover, copper increases the release of PrPC into the culture medium. These results indicate that extracellular copper strongly affects the amount of cellular PrP and might represent an interesting strategy to decrease the expression of PrPC in neurones and its conversion in the pathological isoform PrPSc.

AB - The physiological functions of cellular prion protein (PrPC) remain unclear. It has been demonstrated that PrPC is a copper binding protein and proposed that its functions could be strictly linked to copper metabolism and neuroprotection. The aim of this study was to clarify how extracellular copper modifies PrPC expression and metabolism in cultured neurones. We reported here that copper delivered at physiological concentrations significantly decreases PrPC mRNA expression in GN11 neurones. Moreover, copper increases the release of PrPC into the culture medium. These results indicate that extracellular copper strongly affects the amount of cellular PrP and might represent an interesting strategy to decrease the expression of PrPC in neurones and its conversion in the pathological isoform PrPSc.

KW - Cellular prion protein expression

KW - Cellular prion protein physiology

KW - Copper metabolism

KW - GN11 cell

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Extracellular copper ions regulate cellular prion protein (PrPC) expression and metabolism in neuronal cells

Abstract

Keywords

ASJC Scopus subject areas

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