Extracellular entrapment and degradation of single-walled carbon nanotubes

Consol Farrera, Kunal Bhattacharya, Beatrice Lazzaretto, Fernando T. Andón, Kjell Hultenby, Gregg P. Kotchey, Alexander Star, Bengt Fadeel

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Abstract

Neutrophils extrude neutrophil extracellular traps (NETs) consisting of a network of chromatin decorated with antimicrobial proteins to enable non-phagocytic killing of microorganisms. Here, utilizing a model of ex vivo activated human neutrophils, we present evidence of entrapment and degradation of carboxylated single-walled carbon nanotubes (SWCNTs) in NETs. The degradation of SWCNTs was catalyzed by myeloperoxidase (MPO) present in purified NETs and the reaction was facilitated by the addition of H2O2 and NaBr. These results show that SWCNTs can undergo acellular, MPO-mediated biodegradation and imply that the immune system may deploy similar strategies to rid the body of offending microorganisms and engineered nanomaterials.

Original languageEnglish
Pages (from-to)6974-6983
Number of pages10
JournalNanoscale
Volume6
Issue number12
DOIs
Publication statusPublished - Jun 21 2014

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ASJC Scopus subject areas

  • Materials Science(all)
  • Medicine(all)

Cite this

Farrera, C., Bhattacharya, K., Lazzaretto, B., Andón, F. T., Hultenby, K., Kotchey, G. P., Star, A., & Fadeel, B. (2014). Extracellular entrapment and degradation of single-walled carbon nanotubes. Nanoscale, 6(12), 6974-6983. https://doi.org/10.1039/c3nr06047k