Extracellular entrapment and degradation of single-walled carbon nanotubes

Consol Farrera; Kunal Bhattacharya; Beatrice Lazzaretto; Fernando T. Andón; Kjell Hultenby; Gregg P. Kotchey; Alexander Star; Bengt Fadeel

doi:10.1039/c3nr06047k

Extracellular entrapment and degradation of single-walled carbon nanotubes

Consol Farrera, Kunal Bhattacharya, Beatrice Lazzaretto, Fernando T. Andón, Kjell Hultenby, Gregg P. Kotchey, Alexander Star, Bengt Fadeel

Istituto Clinico Humanitas

Research output: Contribution to journal › Article › peer-review

Abstract

Neutrophils extrude neutrophil extracellular traps (NETs) consisting of a network of chromatin decorated with antimicrobial proteins to enable non-phagocytic killing of microorganisms. Here, utilizing a model of ex vivo activated human neutrophils, we present evidence of entrapment and degradation of carboxylated single-walled carbon nanotubes (SWCNTs) in NETs. The degradation of SWCNTs was catalyzed by myeloperoxidase (MPO) present in purified NETs and the reaction was facilitated by the addition of H₂O₂ and NaBr. These results show that SWCNTs can undergo acellular, MPO-mediated biodegradation and imply that the immune system may deploy similar strategies to rid the body of offending microorganisms and engineered nanomaterials.

Original language	English
Pages (from-to)	6974-6983
Number of pages	10
Journal	Nanoscale
Volume	6
Issue number	12
DOIs	https://doi.org/10.1039/c3nr06047k
Publication status	Published - Jun 21 2014

ASJC Scopus subject areas

Materials Science(all)
Medicine(all)

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10.1039/c3nr06047k

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abstract = "Neutrophils extrude neutrophil extracellular traps (NETs) consisting of a network of chromatin decorated with antimicrobial proteins to enable non-phagocytic killing of microorganisms. Here, utilizing a model of ex vivo activated human neutrophils, we present evidence of entrapment and degradation of carboxylated single-walled carbon nanotubes (SWCNTs) in NETs. The degradation of SWCNTs was catalyzed by myeloperoxidase (MPO) present in purified NETs and the reaction was facilitated by the addition of H2O2 and NaBr. These results show that SWCNTs can undergo acellular, MPO-mediated biodegradation and imply that the immune system may deploy similar strategies to rid the body of offending microorganisms and engineered nanomaterials.",

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AU - Farrera, Consol

AU - Bhattacharya, Kunal

AU - Lazzaretto, Beatrice

AU - Andón, Fernando T.

AU - Hultenby, Kjell

AU - Kotchey, Gregg P.

AU - Star, Alexander

AU - Fadeel, Bengt

PY - 2014/6/21

Y1 - 2014/6/21

N2 - Neutrophils extrude neutrophil extracellular traps (NETs) consisting of a network of chromatin decorated with antimicrobial proteins to enable non-phagocytic killing of microorganisms. Here, utilizing a model of ex vivo activated human neutrophils, we present evidence of entrapment and degradation of carboxylated single-walled carbon nanotubes (SWCNTs) in NETs. The degradation of SWCNTs was catalyzed by myeloperoxidase (MPO) present in purified NETs and the reaction was facilitated by the addition of H2O2 and NaBr. These results show that SWCNTs can undergo acellular, MPO-mediated biodegradation and imply that the immune system may deploy similar strategies to rid the body of offending microorganisms and engineered nanomaterials.

AB - Neutrophils extrude neutrophil extracellular traps (NETs) consisting of a network of chromatin decorated with antimicrobial proteins to enable non-phagocytic killing of microorganisms. Here, utilizing a model of ex vivo activated human neutrophils, we present evidence of entrapment and degradation of carboxylated single-walled carbon nanotubes (SWCNTs) in NETs. The degradation of SWCNTs was catalyzed by myeloperoxidase (MPO) present in purified NETs and the reaction was facilitated by the addition of H2O2 and NaBr. These results show that SWCNTs can undergo acellular, MPO-mediated biodegradation and imply that the immune system may deploy similar strategies to rid the body of offending microorganisms and engineered nanomaterials.

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Extracellular entrapment and degradation of single-walled carbon nanotubes

Abstract

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