Abstract
Neutrophils extrude neutrophil extracellular traps (NETs) consisting of a network of chromatin decorated with antimicrobial proteins to enable non-phagocytic killing of microorganisms. Here, utilizing a model of ex vivo activated human neutrophils, we present evidence of entrapment and degradation of carboxylated single-walled carbon nanotubes (SWCNTs) in NETs. The degradation of SWCNTs was catalyzed by myeloperoxidase (MPO) present in purified NETs and the reaction was facilitated by the addition of H2O2 and NaBr. These results show that SWCNTs can undergo acellular, MPO-mediated biodegradation and imply that the immune system may deploy similar strategies to rid the body of offending microorganisms and engineered nanomaterials.
| Original language | English |
|---|---|
| Pages (from-to) | 6974-6983 |
| Number of pages | 10 |
| Journal | Nanoscale |
| Volume | 6 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Jun 21 2014 |
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ASJC Scopus subject areas
- Materials Science(all)
- Medicine(all)
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Extracellular entrapment and degradation of single-walled carbon nanotubes. / Farrera, Consol; Bhattacharya, Kunal; Lazzaretto, Beatrice; Andón, Fernando T.; Hultenby, Kjell; Kotchey, Gregg P.; Star, Alexander; Fadeel, Bengt.
In: Nanoscale, Vol. 6, No. 12, 21.06.2014, p. 6974-6983.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Extracellular entrapment and degradation of single-walled carbon nanotubes
AU - Farrera, Consol
AU - Bhattacharya, Kunal
AU - Lazzaretto, Beatrice
AU - Andón, Fernando T.
AU - Hultenby, Kjell
AU - Kotchey, Gregg P.
AU - Star, Alexander
AU - Fadeel, Bengt
PY - 2014/6/21
Y1 - 2014/6/21
N2 - Neutrophils extrude neutrophil extracellular traps (NETs) consisting of a network of chromatin decorated with antimicrobial proteins to enable non-phagocytic killing of microorganisms. Here, utilizing a model of ex vivo activated human neutrophils, we present evidence of entrapment and degradation of carboxylated single-walled carbon nanotubes (SWCNTs) in NETs. The degradation of SWCNTs was catalyzed by myeloperoxidase (MPO) present in purified NETs and the reaction was facilitated by the addition of H2O2 and NaBr. These results show that SWCNTs can undergo acellular, MPO-mediated biodegradation and imply that the immune system may deploy similar strategies to rid the body of offending microorganisms and engineered nanomaterials.
AB - Neutrophils extrude neutrophil extracellular traps (NETs) consisting of a network of chromatin decorated with antimicrobial proteins to enable non-phagocytic killing of microorganisms. Here, utilizing a model of ex vivo activated human neutrophils, we present evidence of entrapment and degradation of carboxylated single-walled carbon nanotubes (SWCNTs) in NETs. The degradation of SWCNTs was catalyzed by myeloperoxidase (MPO) present in purified NETs and the reaction was facilitated by the addition of H2O2 and NaBr. These results show that SWCNTs can undergo acellular, MPO-mediated biodegradation and imply that the immune system may deploy similar strategies to rid the body of offending microorganisms and engineered nanomaterials.
UR - http://www.scopus.com/inward/record.url?scp=84901758784&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901758784&partnerID=8YFLogxK
U2 - 10.1039/c3nr06047k
DO - 10.1039/c3nr06047k
M3 - Article
C2 - 24835568
AN - SCOPUS:84901758784
VL - 6
SP - 6974
EP - 6983
JO - Nanoscale
JF - Nanoscale
SN - 2040-3364
IS - 12
ER -