Extracellular entrapment and degradation of single-walled carbon nanotubes

Consol Farrera; Kunal Bhattacharya; Beatrice Lazzaretto; Fernando T. Andón; Kjell Hultenby; Gregg P. Kotchey; Alexander Star; Bengt Fadeel

doi:10.1039/c3nr06047k

Extracellular entrapment and degradation of single-walled carbon nanotubes

Consol Farrera, Kunal Bhattacharya, Beatrice Lazzaretto, Fernando T. Andón, Kjell Hultenby, Gregg P. Kotchey, Alexander Star, Bengt Fadeel

Istituto Clinico Humanitas

Research output: Contribution to journal › Article

Abstract

Neutrophils extrude neutrophil extracellular traps (NETs) consisting of a network of chromatin decorated with antimicrobial proteins to enable non-phagocytic killing of microorganisms. Here, utilizing a model of ex vivo activated human neutrophils, we present evidence of entrapment and degradation of carboxylated single-walled carbon nanotubes (SWCNTs) in NETs. The degradation of SWCNTs was catalyzed by myeloperoxidase (MPO) present in purified NETs and the reaction was facilitated by the addition of H₂O₂ and NaBr. These results show that SWCNTs can undergo acellular, MPO-mediated biodegradation and imply that the immune system may deploy similar strategies to rid the body of offending microorganisms and engineered nanomaterials.

Original language	English
Pages (from-to)	6974-6983
Number of pages	10
Journal	Nanoscale
Volume	6
Issue number	12
DOIs	https://doi.org/10.1039/c3nr06047k
Publication status	Published - Jun 21 2014

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ASJC Scopus subject areas

Materials Science(all)
Medicine(all)

Cite this

Farrera, C., Bhattacharya, K., Lazzaretto, B., Andón, F. T., Hultenby, K., Kotchey, G. P., Star, A., & Fadeel, B. (2014). Extracellular entrapment and degradation of single-walled carbon nanotubes. Nanoscale, 6(12), 6974-6983. https://doi.org/10.1039/c3nr06047k

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AU - Bhattacharya, Kunal

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AU - Hultenby, Kjell

AU - Kotchey, Gregg P.

AU - Star, Alexander

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10.1039/c3nr06047k