Abstract
A case of cirrhotic liver harbouring three atypical macroregenerative nodules and an hepatocellular carcinoma was immunocytochemically investigated for the expression of VLA-β 1, VLA-α2 and VLA-α5 integrins and for different extracellular matrix (ECM) components (collagen I, collagen IV, laminin, fibronectin and tenascin). In addition, the proliferative activity within the nodules was evaluated, using the MIB 1 monoclonal antibody (MAb). The cirrhotic liver disclosed a continuous staining pattern of the ECM proteins investigated, as well as a "sinusoidal" immunostaining of VLA-β1, VLA-α2 and VLA-α5. The macroregenerative nodules showed a discontinued immunoreactivity for ECM proteins while maintaining a VLA-β1 sinusoidal immunostaining, coupled with intercellular immunostaining. VLA-α2 and VLA-α5 expression was lacking. The growth fraction was low in both the above pathological conditions. The hepatocellular carcinoma was devoid of any ECM immunostaining. VLA-β1 immunoreactivity exhibited a honeycomb pattern of staining, whereas VLA α subunits were absent. MIB1 expression was high, being present in 30% of neoplastic nuclei. A possible relationship between atypical macroregenerative nodules and hepatocellular carcinoma is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 29-36 |
| Number of pages | 8 |
| Journal | Histochemistry |
| Volume | 102 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Aug 1994 |
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- Anatomy
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Extracellular matrix proteins, integrin receptors (VLA-β1, VLA-α2 and VLA-α5) and growth fraction in atypical macroregenerative nodules of the liver : an immunocytochemical case study. / Patriarca, C.; Roncalli, M.; Viale, G.; Alfano, R. M.; Braidotti, P.; Guddo, F.; Coggi, G.
In: Histochemistry, Vol. 102, No. 1, 08.1994, p. 29-36.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Extracellular matrix proteins, integrin receptors (VLA-β1, VLA-α2 and VLA-α5) and growth fraction in atypical macroregenerative nodules of the liver
T2 - an immunocytochemical case study
AU - Patriarca, C.
AU - Roncalli, M.
AU - Viale, G.
AU - Alfano, R. M.
AU - Braidotti, P.
AU - Guddo, F.
AU - Coggi, G.
PY - 1994/8
Y1 - 1994/8
N2 - A case of cirrhotic liver harbouring three atypical macroregenerative nodules and an hepatocellular carcinoma was immunocytochemically investigated for the expression of VLA-β 1, VLA-α2 and VLA-α5 integrins and for different extracellular matrix (ECM) components (collagen I, collagen IV, laminin, fibronectin and tenascin). In addition, the proliferative activity within the nodules was evaluated, using the MIB 1 monoclonal antibody (MAb). The cirrhotic liver disclosed a continuous staining pattern of the ECM proteins investigated, as well as a "sinusoidal" immunostaining of VLA-β1, VLA-α2 and VLA-α5. The macroregenerative nodules showed a discontinued immunoreactivity for ECM proteins while maintaining a VLA-β1 sinusoidal immunostaining, coupled with intercellular immunostaining. VLA-α2 and VLA-α5 expression was lacking. The growth fraction was low in both the above pathological conditions. The hepatocellular carcinoma was devoid of any ECM immunostaining. VLA-β1 immunoreactivity exhibited a honeycomb pattern of staining, whereas VLA α subunits were absent. MIB1 expression was high, being present in 30% of neoplastic nuclei. A possible relationship between atypical macroregenerative nodules and hepatocellular carcinoma is discussed.
AB - A case of cirrhotic liver harbouring three atypical macroregenerative nodules and an hepatocellular carcinoma was immunocytochemically investigated for the expression of VLA-β 1, VLA-α2 and VLA-α5 integrins and for different extracellular matrix (ECM) components (collagen I, collagen IV, laminin, fibronectin and tenascin). In addition, the proliferative activity within the nodules was evaluated, using the MIB 1 monoclonal antibody (MAb). The cirrhotic liver disclosed a continuous staining pattern of the ECM proteins investigated, as well as a "sinusoidal" immunostaining of VLA-β1, VLA-α2 and VLA-α5. The macroregenerative nodules showed a discontinued immunoreactivity for ECM proteins while maintaining a VLA-β1 sinusoidal immunostaining, coupled with intercellular immunostaining. VLA-α2 and VLA-α5 expression was lacking. The growth fraction was low in both the above pathological conditions. The hepatocellular carcinoma was devoid of any ECM immunostaining. VLA-β1 immunoreactivity exhibited a honeycomb pattern of staining, whereas VLA α subunits were absent. MIB1 expression was high, being present in 30% of neoplastic nuclei. A possible relationship between atypical macroregenerative nodules and hepatocellular carcinoma is discussed.
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U2 - 10.1007/BF00271046
DO - 10.1007/BF00271046
M3 - Article
VL - 102
SP - 29
EP - 36
JO - Histochemistry and Cell Biology
JF - Histochemistry and Cell Biology
SN - 0948-6143
IS - 1
ER -