Extracellular Nanovesicles Secreted by Human Osteosarcoma Cells Promote Angiogenesis

Francesca Perut, Laura Roncuzzi, Nicoletta Zini, Annamaria Massa, Nicola Baldini

Research output: Contribution to journalArticle

Abstract

Angiogenesis involves a number of different players among which extracellular nanovesicles (EVs) have recently been proposed as an efficient cargo of pro-angiogenic mediators. Angiogenesis plays a key role in osteosarcoma (OS) development and progression. Acidity is a hallmark of malignancy in a variety of cancers, including sarcomas, as a result of an increased energetic metabolism. The aim of this study was to investigate the role of EVs derived from osteosarcoma cells on angiogenesis and whether extracellular acidity, generated by tumor metabolism, could influence EVs activity. For this purpose, we purified and characterized EVs from OS cells maintained at either acidic or neutral pH. The ability of EVs to induce angiogenesis was assessed in vitro by endothelial cell tube formation and in vivo using chicken chorioallantoic membrane. Our findings demonstrated that EVs derived from osteosarcoma cells maintained either in acidic or neutral conditions induced angiogenesis. The results showed that miRNA and protein content of EVs cargo are correlated with pro-angiogenic activity and this activity is increased by the acidity of tumor microenvironment. This study provides evidence that EVs released by human osteosarcoma cells act as carriers of active angiogenic stimuli that are able to promote endothelial cell functions relevant to angiogenesis.

Original languageEnglish
Number of pages17
JournalCancers
Volume11
Issue number6
DOIs
Publication statusPublished - Jun 5 2019

Fingerprint

Osteosarcoma
Endothelial Cells
Chorioallantoic Membrane
Neoplasms
Tumor Microenvironment
MicroRNAs
Sarcoma
Chickens
Proteins

Keywords

  • acidity
  • angiogenesis
  • extracellular nanovesicles
  • osteosarcoma

Cite this

Extracellular Nanovesicles Secreted by Human Osteosarcoma Cells Promote Angiogenesis. / Perut, Francesca; Roncuzzi, Laura; Zini, Nicoletta; Massa, Annamaria; Baldini, Nicola.

In: Cancers, Vol. 11, No. 6, 05.06.2019.

Research output: Contribution to journalArticle

@article{3c67dc37628f4a5f936ce6ad6a346c9a,
title = "Extracellular Nanovesicles Secreted by Human Osteosarcoma Cells Promote Angiogenesis",
abstract = "Angiogenesis involves a number of different players among which extracellular nanovesicles (EVs) have recently been proposed as an efficient cargo of pro-angiogenic mediators. Angiogenesis plays a key role in osteosarcoma (OS) development and progression. Acidity is a hallmark of malignancy in a variety of cancers, including sarcomas, as a result of an increased energetic metabolism. The aim of this study was to investigate the role of EVs derived from osteosarcoma cells on angiogenesis and whether extracellular acidity, generated by tumor metabolism, could influence EVs activity. For this purpose, we purified and characterized EVs from OS cells maintained at either acidic or neutral pH. The ability of EVs to induce angiogenesis was assessed in vitro by endothelial cell tube formation and in vivo using chicken chorioallantoic membrane. Our findings demonstrated that EVs derived from osteosarcoma cells maintained either in acidic or neutral conditions induced angiogenesis. The results showed that miRNA and protein content of EVs cargo are correlated with pro-angiogenic activity and this activity is increased by the acidity of tumor microenvironment. This study provides evidence that EVs released by human osteosarcoma cells act as carriers of active angiogenic stimuli that are able to promote endothelial cell functions relevant to angiogenesis.",
keywords = "acidity, angiogenesis, extracellular nanovesicles, osteosarcoma",
author = "Francesca Perut and Laura Roncuzzi and Nicoletta Zini and Annamaria Massa and Nicola Baldini",
year = "2019",
month = "6",
day = "5",
doi = "10.3390/cancers11060779",
language = "English",
volume = "11",
journal = "Cancers",
issn = "2072-6694",
publisher = "MDPI AG",
number = "6",

}

TY - JOUR

T1 - Extracellular Nanovesicles Secreted by Human Osteosarcoma Cells Promote Angiogenesis

AU - Perut, Francesca

AU - Roncuzzi, Laura

AU - Zini, Nicoletta

AU - Massa, Annamaria

AU - Baldini, Nicola

PY - 2019/6/5

Y1 - 2019/6/5

N2 - Angiogenesis involves a number of different players among which extracellular nanovesicles (EVs) have recently been proposed as an efficient cargo of pro-angiogenic mediators. Angiogenesis plays a key role in osteosarcoma (OS) development and progression. Acidity is a hallmark of malignancy in a variety of cancers, including sarcomas, as a result of an increased energetic metabolism. The aim of this study was to investigate the role of EVs derived from osteosarcoma cells on angiogenesis and whether extracellular acidity, generated by tumor metabolism, could influence EVs activity. For this purpose, we purified and characterized EVs from OS cells maintained at either acidic or neutral pH. The ability of EVs to induce angiogenesis was assessed in vitro by endothelial cell tube formation and in vivo using chicken chorioallantoic membrane. Our findings demonstrated that EVs derived from osteosarcoma cells maintained either in acidic or neutral conditions induced angiogenesis. The results showed that miRNA and protein content of EVs cargo are correlated with pro-angiogenic activity and this activity is increased by the acidity of tumor microenvironment. This study provides evidence that EVs released by human osteosarcoma cells act as carriers of active angiogenic stimuli that are able to promote endothelial cell functions relevant to angiogenesis.

AB - Angiogenesis involves a number of different players among which extracellular nanovesicles (EVs) have recently been proposed as an efficient cargo of pro-angiogenic mediators. Angiogenesis plays a key role in osteosarcoma (OS) development and progression. Acidity is a hallmark of malignancy in a variety of cancers, including sarcomas, as a result of an increased energetic metabolism. The aim of this study was to investigate the role of EVs derived from osteosarcoma cells on angiogenesis and whether extracellular acidity, generated by tumor metabolism, could influence EVs activity. For this purpose, we purified and characterized EVs from OS cells maintained at either acidic or neutral pH. The ability of EVs to induce angiogenesis was assessed in vitro by endothelial cell tube formation and in vivo using chicken chorioallantoic membrane. Our findings demonstrated that EVs derived from osteosarcoma cells maintained either in acidic or neutral conditions induced angiogenesis. The results showed that miRNA and protein content of EVs cargo are correlated with pro-angiogenic activity and this activity is increased by the acidity of tumor microenvironment. This study provides evidence that EVs released by human osteosarcoma cells act as carriers of active angiogenic stimuli that are able to promote endothelial cell functions relevant to angiogenesis.

KW - acidity

KW - angiogenesis

KW - extracellular nanovesicles

KW - osteosarcoma

U2 - 10.3390/cancers11060779

DO - 10.3390/cancers11060779

M3 - Article

VL - 11

JO - Cancers

JF - Cancers

SN - 2072-6694

IS - 6

ER -