Extracellular proteasome-osteopontin circuit regulates cell migration with implications in multiple sclerosis

Chiara Dianzani, Elena Bellavista, Juliane Liepe, Claudia Verderio, Morena Martucci, Aurelia Santoro, Annalisa Chiocchetti, Casimiro Luca Gigliotti, Elena Boggio, Benedetta Ferrara, Loredana Riganti, Christin Keller, Katharina Janek, Agathe Niewienda, Chiara Fenoglio, Melissa Sorosina, Roberto Cantello, Peter M Kloetzel, Michael P H Stumpf, Friedemann Paul & 6 others Klemens Ruprecht, Daniela Galimberti, Filippo Martinelli Boneschi, Cristoforo Comi, Umberto Dianzani, Michele Mishto

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Osteopontin is a pleiotropic cytokine that is involved in several diseases including multiple sclerosis. Secreted osteopontin is cleaved by few known proteases, modulating its pro-inflammatory activities. Here we show by in vitro experiments that secreted osteopontin can be processed by extracellular proteasomes, thereby producing fragments with novel chemotactic activity. Furthermore, osteopontin reduces the release of proteasomes in the extracellular space. The latter phenomenon seems to occur in vivo in multiple sclerosis, where it reflects the remission/relapse alternation. The extracellular proteasome-mediated inflammatory pathway may represent a general mechanism to control inflammation in inflammatory diseases.

Original languageEnglish
Article number43718
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - Mar 9 2017

Fingerprint

Osteopontin
Proteasome Endopeptidase Complex
Multiple Sclerosis
Cell Movement
Extracellular Space
Peptide Hydrolases
Cytokines
Inflammation
Recurrence

Keywords

  • Journal Article

Cite this

Extracellular proteasome-osteopontin circuit regulates cell migration with implications in multiple sclerosis. / Dianzani, Chiara; Bellavista, Elena; Liepe, Juliane; Verderio, Claudia; Martucci, Morena; Santoro, Aurelia; Chiocchetti, Annalisa; Gigliotti, Casimiro Luca; Boggio, Elena; Ferrara, Benedetta; Riganti, Loredana; Keller, Christin; Janek, Katharina; Niewienda, Agathe; Fenoglio, Chiara; Sorosina, Melissa; Cantello, Roberto; Kloetzel, Peter M; Stumpf, Michael P H; Paul, Friedemann; Ruprecht, Klemens; Galimberti, Daniela; Martinelli Boneschi, Filippo; Comi, Cristoforo; Dianzani, Umberto; Mishto, Michele.

In: Scientific Reports, Vol. 7, 43718, 09.03.2017.

Research output: Contribution to journalArticle

Dianzani, C, Bellavista, E, Liepe, J, Verderio, C, Martucci, M, Santoro, A, Chiocchetti, A, Gigliotti, CL, Boggio, E, Ferrara, B, Riganti, L, Keller, C, Janek, K, Niewienda, A, Fenoglio, C, Sorosina, M, Cantello, R, Kloetzel, PM, Stumpf, MPH, Paul, F, Ruprecht, K, Galimberti, D, Martinelli Boneschi, F, Comi, C, Dianzani, U & Mishto, M 2017, 'Extracellular proteasome-osteopontin circuit regulates cell migration with implications in multiple sclerosis', Scientific Reports, vol. 7, 43718. https://doi.org/10.1038/srep43718
Dianzani, Chiara ; Bellavista, Elena ; Liepe, Juliane ; Verderio, Claudia ; Martucci, Morena ; Santoro, Aurelia ; Chiocchetti, Annalisa ; Gigliotti, Casimiro Luca ; Boggio, Elena ; Ferrara, Benedetta ; Riganti, Loredana ; Keller, Christin ; Janek, Katharina ; Niewienda, Agathe ; Fenoglio, Chiara ; Sorosina, Melissa ; Cantello, Roberto ; Kloetzel, Peter M ; Stumpf, Michael P H ; Paul, Friedemann ; Ruprecht, Klemens ; Galimberti, Daniela ; Martinelli Boneschi, Filippo ; Comi, Cristoforo ; Dianzani, Umberto ; Mishto, Michele. / Extracellular proteasome-osteopontin circuit regulates cell migration with implications in multiple sclerosis. In: Scientific Reports. 2017 ; Vol. 7.
@article{11db67c5c057442baf9b3823b860c2d4,
title = "Extracellular proteasome-osteopontin circuit regulates cell migration with implications in multiple sclerosis",
abstract = "Osteopontin is a pleiotropic cytokine that is involved in several diseases including multiple sclerosis. Secreted osteopontin is cleaved by few known proteases, modulating its pro-inflammatory activities. Here we show by in vitro experiments that secreted osteopontin can be processed by extracellular proteasomes, thereby producing fragments with novel chemotactic activity. Furthermore, osteopontin reduces the release of proteasomes in the extracellular space. The latter phenomenon seems to occur in vivo in multiple sclerosis, where it reflects the remission/relapse alternation. The extracellular proteasome-mediated inflammatory pathway may represent a general mechanism to control inflammation in inflammatory diseases.",
keywords = "Journal Article",
author = "Chiara Dianzani and Elena Bellavista and Juliane Liepe and Claudia Verderio and Morena Martucci and Aurelia Santoro and Annalisa Chiocchetti and Gigliotti, {Casimiro Luca} and Elena Boggio and Benedetta Ferrara and Loredana Riganti and Christin Keller and Katharina Janek and Agathe Niewienda and Chiara Fenoglio and Melissa Sorosina and Roberto Cantello and Kloetzel, {Peter M} and Stumpf, {Michael P H} and Friedemann Paul and Klemens Ruprecht and Daniela Galimberti and {Martinelli Boneschi}, Filippo and Cristoforo Comi and Umberto Dianzani and Michele Mishto",
year = "2017",
month = "3",
day = "9",
doi = "10.1038/srep43718",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Extracellular proteasome-osteopontin circuit regulates cell migration with implications in multiple sclerosis

AU - Dianzani, Chiara

AU - Bellavista, Elena

AU - Liepe, Juliane

AU - Verderio, Claudia

AU - Martucci, Morena

AU - Santoro, Aurelia

AU - Chiocchetti, Annalisa

AU - Gigliotti, Casimiro Luca

AU - Boggio, Elena

AU - Ferrara, Benedetta

AU - Riganti, Loredana

AU - Keller, Christin

AU - Janek, Katharina

AU - Niewienda, Agathe

AU - Fenoglio, Chiara

AU - Sorosina, Melissa

AU - Cantello, Roberto

AU - Kloetzel, Peter M

AU - Stumpf, Michael P H

AU - Paul, Friedemann

AU - Ruprecht, Klemens

AU - Galimberti, Daniela

AU - Martinelli Boneschi, Filippo

AU - Comi, Cristoforo

AU - Dianzani, Umberto

AU - Mishto, Michele

PY - 2017/3/9

Y1 - 2017/3/9

N2 - Osteopontin is a pleiotropic cytokine that is involved in several diseases including multiple sclerosis. Secreted osteopontin is cleaved by few known proteases, modulating its pro-inflammatory activities. Here we show by in vitro experiments that secreted osteopontin can be processed by extracellular proteasomes, thereby producing fragments with novel chemotactic activity. Furthermore, osteopontin reduces the release of proteasomes in the extracellular space. The latter phenomenon seems to occur in vivo in multiple sclerosis, where it reflects the remission/relapse alternation. The extracellular proteasome-mediated inflammatory pathway may represent a general mechanism to control inflammation in inflammatory diseases.

AB - Osteopontin is a pleiotropic cytokine that is involved in several diseases including multiple sclerosis. Secreted osteopontin is cleaved by few known proteases, modulating its pro-inflammatory activities. Here we show by in vitro experiments that secreted osteopontin can be processed by extracellular proteasomes, thereby producing fragments with novel chemotactic activity. Furthermore, osteopontin reduces the release of proteasomes in the extracellular space. The latter phenomenon seems to occur in vivo in multiple sclerosis, where it reflects the remission/relapse alternation. The extracellular proteasome-mediated inflammatory pathway may represent a general mechanism to control inflammation in inflammatory diseases.

KW - Journal Article

U2 - 10.1038/srep43718

DO - 10.1038/srep43718

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 43718

ER -