TY - JOUR
T1 - Extracellular vesicles derived from human gingival mesenchymal stem cells
T2 - A transcriptomic analysis
AU - Silvestro, Serena
AU - Chiricosta, Luigi
AU - Gugliandolo, Agnese
AU - Pizzicannella, Jacopo
AU - Diomede, Francesca
AU - Bramanti, Placido
AU - Trubiani, Oriana
AU - Mazzon, Emanuela
N1 - Funding Information:
Funding: This study was supported by a Current Research Fund 2019, Ministry of Health, Italy.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Human gingival mesenchymal stem cells (hGMSCs) have outstanding characteristics of proliferation and are able to differentiate into osteogenic, chondrogenic, adipogenic, and neurogenic cell lineages. The extracellular vesicles (EVs) secreted by hGMSCs contain proteins, lipids, mRNA and microRNA have emerged as important mediators of cell‐to‐cell communication. In this study, we analyzed the transcriptome of hGMSCs‐derived EVs using Next Generation Sequencing (NGS). The functional evaluation of the transcriptome highlighted 26 structural protein classes and the presence of “non‐coding RNAs”. Our results showed that EVs contain several growth factors such as Transforming Growth Factor‐β (TGF‐β), Fibroblast Growth Factor (FGF), and Vascular Endothelial Growth Factors (VEGF) implicated in osteoblast differentiation and in angiogenetic process. Furthermore, the transcriptomic analysis showed the presence of glial cell‐derived neurotrophic factor (GDNF) family ligands and neurotrophins involved in neuronal development. The NGS analysis also identified the presence of several interleukins among which some with anti‐inflammatory action. Moreover, the transcriptome profile of EVs contained members of the Wnt family, involved in several biological processes, such as cellular proliferation and tissue regeneration. In conclusion, the huge amount of growth factors included in the hGMSCs‐derived EVs could make them a big resource in regenerative medicine.
AB - Human gingival mesenchymal stem cells (hGMSCs) have outstanding characteristics of proliferation and are able to differentiate into osteogenic, chondrogenic, adipogenic, and neurogenic cell lineages. The extracellular vesicles (EVs) secreted by hGMSCs contain proteins, lipids, mRNA and microRNA have emerged as important mediators of cell‐to‐cell communication. In this study, we analyzed the transcriptome of hGMSCs‐derived EVs using Next Generation Sequencing (NGS). The functional evaluation of the transcriptome highlighted 26 structural protein classes and the presence of “non‐coding RNAs”. Our results showed that EVs contain several growth factors such as Transforming Growth Factor‐β (TGF‐β), Fibroblast Growth Factor (FGF), and Vascular Endothelial Growth Factors (VEGF) implicated in osteoblast differentiation and in angiogenetic process. Furthermore, the transcriptomic analysis showed the presence of glial cell‐derived neurotrophic factor (GDNF) family ligands and neurotrophins involved in neuronal development. The NGS analysis also identified the presence of several interleukins among which some with anti‐inflammatory action. Moreover, the transcriptome profile of EVs contained members of the Wnt family, involved in several biological processes, such as cellular proliferation and tissue regeneration. In conclusion, the huge amount of growth factors included in the hGMSCs‐derived EVs could make them a big resource in regenerative medicine.
KW - Extracellular vesicles
KW - Human gingival mesenchymal stem cells
KW - Next generation sequencing
KW - Transciptome
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U2 - 10.3390/genes11020118
DO - 10.3390/genes11020118
M3 - Article
C2 - 31973135
AN - SCOPUS:85078243967
VL - 11
JO - Genes
JF - Genes
SN - 2073-4425
IS - 2
M1 - 118
ER -