Extrachromosomal genes: A powerful tool in gene targeting approaches

A. Colosimo, V. Guida, G. Palka, B. Dallapiccola

Research output: Contribution to journalArticle

Abstract

Several studies, some of which have been updated during the recent workshop entitled Genome Medicine: Gene Therapy for the Millennium (Rome, 30 September-3 October 2001), have highlighted the usefulness of extrachromosomal or episomal genes in gene targeting strategies. Due to the selectable nature of antibiotic resistance and reporter genes, targeted correction of mutated versions of these extrachromosomal genes allows an accurate quantification of correction frequency. In addition, these model systems facilitate and speed up the optimization of critical parameters for the successful application of gene targeting approaches. In fact, type of cell line, gene delivery system, molar ratio of episomal target/therapeutic constructs, nature and design of therapeutic complexes and different recombinative proteins may be critical for the actual feasibility of each method. Although virus-based approaches are now being investigated as well, this article is focusing on the targeted correction of extrachromosomal genes by the use of small DNA fragments (SDF), chimeric RNA/DNA oligonucleotides (RDO) and triplex-forming oligonucleotides (TFO).

Original languageEnglish
Pages (from-to)679-682
Number of pages4
JournalGene Therapy
Volume9
Issue number11
DOIs
Publication statusPublished - 2002

Fingerprint

Gene Targeting
Oligonucleotides
Targeted Gene Repair
Gene Transfer Techniques
Microbial Drug Resistance
Reporter Genes
Genetic Therapy
Genes
Medicine
Genome
RNA
Viruses
Education
Cell Line
DNA
Therapeutics
Proteins
triplex DNA

Keywords

  • Correction frequency
  • Delivery systems
  • Extrachromosomal genes
  • Gene targeting

ASJC Scopus subject areas

  • Genetics

Cite this

Extrachromosomal genes : A powerful tool in gene targeting approaches. / Colosimo, A.; Guida, V.; Palka, G.; Dallapiccola, B.

In: Gene Therapy, Vol. 9, No. 11, 2002, p. 679-682.

Research output: Contribution to journalArticle

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