TY - JOUR
T1 - Extracorporeal photochemotherapy is accompanied by increasing levels of circulating CD4+CD25+GITR+Foxp3+CD62L+ functional regulatory T-cells in patients with graft-versus-host disease
AU - Biagi, Ettore
AU - Di Biaso, Iolanda
AU - Leoni, Veronica
AU - Gaipa, Giuseppe
AU - Rossi, Vincenzo
AU - Bugarin, Cristina
AU - Renoldi, Giuliano
AU - Parma, Matteo
AU - Balduzzi, Adriana
AU - Perseghin, Paolo
AU - Biondi, Andrea
PY - 2007/7
Y1 - 2007/7
N2 - BACKGROUND. Extracorporeal photochemotherapy (ECP) produces clinical improvements in refractory/resistant graft-versus-host disease (GvHD). Immunological mechanisms of ECP are still under investigation. METHODS. We have evaluated the changes in frequency and immunophenotype of circulating regulatory T cells (T-regs) in 10 patients undergoing allogeneic hematopoietic stem cell transplantation, receiving ECP for acute (n=4) or chronic (n=6) GvHD. T-regs were monitored for expression of surface CD4, CD25, GITR, CD45RO, CD62L and intracytoplasmic Foxp3. T-regs were sorted by fluorescence-activated cell sorting to perform functional assays by interferon (IFN)-γ enzyme-linked immunospot and real-time quantitative polymerase chain reaction (RQ-PCR) to measure Foxp3, transforming growth factor (TGF)-β, and interleukin (IL)-10 mRNA. RESULTS. ECP was accompanied by a significant increase of CD4+CD25+ T-regs after six procedures, increasing from 8.9% to 29.1% of total CD4 (P
AB - BACKGROUND. Extracorporeal photochemotherapy (ECP) produces clinical improvements in refractory/resistant graft-versus-host disease (GvHD). Immunological mechanisms of ECP are still under investigation. METHODS. We have evaluated the changes in frequency and immunophenotype of circulating regulatory T cells (T-regs) in 10 patients undergoing allogeneic hematopoietic stem cell transplantation, receiving ECP for acute (n=4) or chronic (n=6) GvHD. T-regs were monitored for expression of surface CD4, CD25, GITR, CD45RO, CD62L and intracytoplasmic Foxp3. T-regs were sorted by fluorescence-activated cell sorting to perform functional assays by interferon (IFN)-γ enzyme-linked immunospot and real-time quantitative polymerase chain reaction (RQ-PCR) to measure Foxp3, transforming growth factor (TGF)-β, and interleukin (IL)-10 mRNA. RESULTS. ECP was accompanied by a significant increase of CD4+CD25+ T-regs after six procedures, increasing from 8.9% to 29.1% of total CD4 (P
KW - Extracorporeal photochemotherapy
KW - Graft-versus-host disease
KW - Hematopoietic stem cell transplantation
KW - Regulatory T cells
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U2 - 10.1097/01.tp.0000267785.52567.9c
DO - 10.1097/01.tp.0000267785.52567.9c
M3 - Article
C2 - 17627234
AN - SCOPUS:34447635261
VL - 84
SP - 31
EP - 39
JO - Transplantation
JF - Transplantation
SN - 0041-1337
IS - 1
ER -