TY - JOUR
T1 - Extraction and partial purification of a CEA-like BCA from a pool of pre-selected breast carcinomas
AU - Accinni, R.
AU - Bailo, M.
AU - Cavalca, V.
AU - Ferrara, R.
AU - Biancardi, C.
AU - Bartorelli, A.
PY - 1982
Y1 - 1982
N2 - By exploiting the cross-reaction among the antigens associated with a pool of breast carcinomas, preselected according to their antigenic content, a CEA-like BCA, with a specific activity of 1400 U/μg, has been extracted, by means of 3M KCl, and partially purified by means of ConA Sepharose affinity chromatography and bioabsorption, employing, for the latter, a cross-reacting antiserum (anti CEA). The subsequent labelling, after Sephadex G-200 gel filtration, points out an immunological activity at the apex of the first peak versus anti CBC (or C.BCA) and anti CEA.
AB - By exploiting the cross-reaction among the antigens associated with a pool of breast carcinomas, preselected according to their antigenic content, a CEA-like BCA, with a specific activity of 1400 U/μg, has been extracted, by means of 3M KCl, and partially purified by means of ConA Sepharose affinity chromatography and bioabsorption, employing, for the latter, a cross-reacting antiserum (anti CEA). The subsequent labelling, after Sephadex G-200 gel filtration, points out an immunological activity at the apex of the first peak versus anti CBC (or C.BCA) and anti CEA.
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M3 - Article
C2 - 7126333
AN - SCOPUS:0020287576
VL - 61
SP - 8
EP - 14
JO - Bollettino dell'Istituto Sieroterapico Milanese
JF - Bollettino dell'Istituto Sieroterapico Milanese
SN - 0021-2547
IS - 1
ER -