Extraction and partial purification of a CEA-like BCA from a pool of pre-selected breast carcinomas

R. Accinni, M. Bailo, V. Cavalca, R. Ferrara, C. Biancardi, A. Bartorelli

Research output: Contribution to journalArticle

Abstract

By exploiting the cross-reaction among the antigens associated with a pool of breast carcinomas, preselected according to their antigenic content, a CEA-like BCA, with a specific activity of 1400 U/μg, has been extracted, by means of 3M KCl, and partially purified by means of ConA Sepharose affinity chromatography and bioabsorption, employing, for the latter, a cross-reacting antiserum (anti CEA). The subsequent labelling, after Sephadex G-200 gel filtration, points out an immunological activity at the apex of the first peak versus anti CBC (or C.BCA) and anti CEA.

Original languageEnglish
Pages (from-to)8-14
Number of pages7
JournalBollettino dell'Istituto Sieroterapico Milanese
Volume61
Issue number1
Publication statusPublished - 1982

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Agarose Chromatography
Cross Reactions
Affinity Chromatography
Gel Chromatography
Immune Sera
Breast Neoplasms
Antigens
sephadex

ASJC Scopus subject areas

  • Immunology

Cite this

Extraction and partial purification of a CEA-like BCA from a pool of pre-selected breast carcinomas. / Accinni, R.; Bailo, M.; Cavalca, V.; Ferrara, R.; Biancardi, C.; Bartorelli, A.

In: Bollettino dell'Istituto Sieroterapico Milanese, Vol. 61, No. 1, 1982, p. 8-14.

Research output: Contribution to journalArticle

Accinni, R. ; Bailo, M. ; Cavalca, V. ; Ferrara, R. ; Biancardi, C. ; Bartorelli, A. / Extraction and partial purification of a CEA-like BCA from a pool of pre-selected breast carcinomas. In: Bollettino dell'Istituto Sieroterapico Milanese. 1982 ; Vol. 61, No. 1. pp. 8-14.
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