Extraction and partial purification of a hepatic stimulatory substance in rats, mice, and dogs

A. Francavilla, P. Ove, L. Polimeno, M. Coetzee, L. Makowka, J. Rose, D. H. Van Thiel, T. E. Starzl

Research output: Contribution to journalArticle

Abstract

A factor has been isolated from weanling rat liver which stimulates in vivo hepatic DNA synthesis in a dose dependent manner when injected into 40% hepatectomized rats. The factor has been partially purified by successive steps, involving ethanol precipitation, ultrafiltration through an Amicon PM 30 membrane, and finally fast protein liquid chromatography, resulting in a 38,000-fold increase in specific activity over that in the original cytosol. The factor contains a few bands in the molecular weight range of 14,000-50,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Active fractions from fast protein liquid chromatography (F150), when injected into 40% hepatectomized rats, increased hepatic DNA synthesis 3-fold over the background stimulation due to the hepatectomy. The response was dose dependent over a range from 1.76 μg to 6.8 μg per 200-g (body weight) rat. Mitotic and labeling indexes confirmed that F150 stimulates both replicative DNA synthesis and cell proliferation. The factor is heat and neuraminidase resistant, trypsin sensitive, organ specific, but not species specific.

Original languageEnglish
Pages (from-to)5600-5605
Number of pages6
JournalCancer Research
Volume47
Issue number21
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Francavilla, A., Ove, P., Polimeno, L., Coetzee, M., Makowka, L., Rose, J., Van Thiel, D. H., & Starzl, T. E. (1987). Extraction and partial purification of a hepatic stimulatory substance in rats, mice, and dogs. Cancer Research, 47(21), 5600-5605.