Extralesional detection and load of human papillomavirus DNA: A possible marker of preclinical tumor spread in cervical cancer

Flavia Lillo, Laura Galli, Sara Lodini, Gianluca Taccagni, Augusto Ferrari, Massimo Origoni

Research output: Contribution to journalArticle

Abstract

Objective. Because the interaction between viral DNA products and cellular regulatory mechanisms is the first step leading to cancerous transformation, the detection of its presence in histologically negative lymph nodes may represent a very early biological step in cancer spread. The quantitative estimate may represent and an indirect sign of active cellular replication. Materials and Methods. Cervical and lymph nodes tissues of 13 cases of invasive cervical cancer were analyzed for human papillomavirus (HPV)-DNA presence and viral load by HPV typing and quantification by real-time polymerase chain reaction. Results. HPV-DNA was demonstrated in all tissue samples (primary tumor, positive lymph nodes, negative lymph nodes) with the most prevalence of HPV 16 (61.5%) and single-type infection (69.3%), whereas viral load (mean quantity of DNA copies) is statistically different in negative versus positive lymph nodes (p =.005). Conclusions.Concordance of viral type and lymph nodes viral load may represent as a useful tool in identifying early metastatic risk of tumor spread.

Original languageEnglish
Pages (from-to)204-209
Number of pages6
JournalJournal of Lower Genital Tract Disease
Volume12
Issue number3
DOIs
Publication statusPublished - Jul 2008

Keywords

  • Cervical cancer
  • HPV
  • HPVDNA
  • Human papillomavirus
  • Lymph node metastases
  • Viral load

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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