Extranodal marginal zone B-cell lymphoma genotyping by Alu-polymerase chain reaction

Francesco Bertoni, Roman Müllenbach, Massimo Broggini, Enrico Roggero, Franco Cavalli, Finbarr E. Cotter, Emanuele Zucca

Research output: Contribution to journalArticlepeer-review

Abstract

DNA amplification by polymerase chain reaction (PCR) with primers designed on the widely distributed Alu sequences allows the production of specific inter-Alu DNA-fingerprints. Amplification of tumour and matched normal DNA can show differences due to genetic alterations within the tumour genome. We applied this approach to study low-grade extranodal marginal zone B-cell lymphoma (of MALT type). After digestion with restriction enzymes, DNA samples were separately amplified by PCR with three different Alu-primers. A comparison between the fingerprint pattern from lymphoma and normal samples was made. Inter-Alu bands differing between the two samples were excised from the gel, cloned and sequenced. Nine cases of low-grade MALT-lymphomas have been analysed, giving seventeen different bands between tumour and normal. DNA sequence analysis showed identities for three of them with sequences available at the GenBank. The methodology of Alu-PCR to detect DNA-based abnormalities, in addition or combination with RNA-based methods, is a powerful tool to identify candidate regions frequently altered in tumours. With the increased available genomic sequences through the Human Genome Project, there will be an increasing probability of picking up perfect homologies with these sequences using cloned differential Alu-PCR bands in BLAST searches through genome databases.

Original languageEnglish
Pages (from-to)605-610
Number of pages6
JournalLeukemia and Lymphoma
Volume38
Issue number5-6
Publication statusPublished - 2000

Keywords

  • Alu
  • Extranodal
  • Genetic lesions
  • MALT
  • Marginal zone B-cell lymphoma
  • PCR

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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