Eye drop NGF administration promotes the recovery of chemically injured cholinergic neurons of adult mouse forebrain

Veronica Di Fausto, Marco Fiore, Paola Tirassa, Alessandro Lambiase, Luigi Aloe

Research output: Contribution to journalArticle

Abstract

We have recently shown that conjunctivally applied nerve growth factor (NGF) in rats can reach the retina, the optic nerve and the CNS. In the present study, we investigated whether NGF application as collyrium can promote the recovery of chemically injured basal forebrain cholinergic neurons. NGF was administered on the eye of adult male mice previously treated i.c.v. with ibotenic acid to impair cholinergic pathways. Mice were tested in the passive avoidance test, and after 2 weeks of NGF administration were killed and the brains used for structural, biochemical and molecular analyses. The results showed that application of NGF on the eye surface protected choline acetyl transferase levels. These findings strengthen the hypothesis that application of NGF on the eye can represent an alternative delivery route to promote the recovery of brain cells during degeneration, including neurons involved in learning and memory activities.

Original languageEnglish
Pages (from-to)2473-2480
Number of pages8
JournalEuropean Journal of Neuroscience
Volume26
Issue number9
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Cholinergic Neurons
Ophthalmic Solutions
Nerve Growth Factor
Prosencephalon
Ibotenic Acid
Neurotrophin 3
Nerve Degeneration
Brain
Optic Nerve
Transferases
Choline
Cholinergic Agents
Retina
Learning

Keywords

  • Behaviour
  • ChAT
  • Ibotenic acid
  • NGF
  • Passive avoidance

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Eye drop NGF administration promotes the recovery of chemically injured cholinergic neurons of adult mouse forebrain. / Di Fausto, Veronica; Fiore, Marco; Tirassa, Paola; Lambiase, Alessandro; Aloe, Luigi.

In: European Journal of Neuroscience, Vol. 26, No. 9, 11.2007, p. 2473-2480.

Research output: Contribution to journalArticle

Di Fausto, Veronica ; Fiore, Marco ; Tirassa, Paola ; Lambiase, Alessandro ; Aloe, Luigi. / Eye drop NGF administration promotes the recovery of chemically injured cholinergic neurons of adult mouse forebrain. In: European Journal of Neuroscience. 2007 ; Vol. 26, No. 9. pp. 2473-2480.
@article{521a58f630954a118655c3619c893cd9,
title = "Eye drop NGF administration promotes the recovery of chemically injured cholinergic neurons of adult mouse forebrain",
abstract = "We have recently shown that conjunctivally applied nerve growth factor (NGF) in rats can reach the retina, the optic nerve and the CNS. In the present study, we investigated whether NGF application as collyrium can promote the recovery of chemically injured basal forebrain cholinergic neurons. NGF was administered on the eye of adult male mice previously treated i.c.v. with ibotenic acid to impair cholinergic pathways. Mice were tested in the passive avoidance test, and after 2 weeks of NGF administration were killed and the brains used for structural, biochemical and molecular analyses. The results showed that application of NGF on the eye surface protected choline acetyl transferase levels. These findings strengthen the hypothesis that application of NGF on the eye can represent an alternative delivery route to promote the recovery of brain cells during degeneration, including neurons involved in learning and memory activities.",
keywords = "Behaviour, ChAT, Ibotenic acid, NGF, Passive avoidance",
author = "{Di Fausto}, Veronica and Marco Fiore and Paola Tirassa and Alessandro Lambiase and Luigi Aloe",
year = "2007",
month = "11",
doi = "10.1111/j.1460-9568.2007.05883.x",
language = "English",
volume = "26",
pages = "2473--2480",
journal = "European Journal of Neuroscience",
issn = "0953-816X",
publisher = "Wiley-Blackwell",
number = "9",

}

TY - JOUR

T1 - Eye drop NGF administration promotes the recovery of chemically injured cholinergic neurons of adult mouse forebrain

AU - Di Fausto, Veronica

AU - Fiore, Marco

AU - Tirassa, Paola

AU - Lambiase, Alessandro

AU - Aloe, Luigi

PY - 2007/11

Y1 - 2007/11

N2 - We have recently shown that conjunctivally applied nerve growth factor (NGF) in rats can reach the retina, the optic nerve and the CNS. In the present study, we investigated whether NGF application as collyrium can promote the recovery of chemically injured basal forebrain cholinergic neurons. NGF was administered on the eye of adult male mice previously treated i.c.v. with ibotenic acid to impair cholinergic pathways. Mice were tested in the passive avoidance test, and after 2 weeks of NGF administration were killed and the brains used for structural, biochemical and molecular analyses. The results showed that application of NGF on the eye surface protected choline acetyl transferase levels. These findings strengthen the hypothesis that application of NGF on the eye can represent an alternative delivery route to promote the recovery of brain cells during degeneration, including neurons involved in learning and memory activities.

AB - We have recently shown that conjunctivally applied nerve growth factor (NGF) in rats can reach the retina, the optic nerve and the CNS. In the present study, we investigated whether NGF application as collyrium can promote the recovery of chemically injured basal forebrain cholinergic neurons. NGF was administered on the eye of adult male mice previously treated i.c.v. with ibotenic acid to impair cholinergic pathways. Mice were tested in the passive avoidance test, and after 2 weeks of NGF administration were killed and the brains used for structural, biochemical and molecular analyses. The results showed that application of NGF on the eye surface protected choline acetyl transferase levels. These findings strengthen the hypothesis that application of NGF on the eye can represent an alternative delivery route to promote the recovery of brain cells during degeneration, including neurons involved in learning and memory activities.

KW - Behaviour

KW - ChAT

KW - Ibotenic acid

KW - NGF

KW - Passive avoidance

UR - http://www.scopus.com/inward/record.url?scp=35748957462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35748957462&partnerID=8YFLogxK

U2 - 10.1111/j.1460-9568.2007.05883.x

DO - 10.1111/j.1460-9568.2007.05883.x

M3 - Article

C2 - 17970722

AN - SCOPUS:35748957462

VL - 26

SP - 2473

EP - 2480

JO - European Journal of Neuroscience

JF - European Journal of Neuroscience

SN - 0953-816X

IS - 9

ER -