Eye drop NGF administration promotes the recovery of chemically injured cholinergic neurons of adult mouse forebrain

Veronica Di Fausto, Marco Fiore, Paola Tirassa, Alessandro Lambiase, Luigi Aloe

Research output: Contribution to journalArticlepeer-review

Abstract

We have recently shown that conjunctivally applied nerve growth factor (NGF) in rats can reach the retina, the optic nerve and the CNS. In the present study, we investigated whether NGF application as collyrium can promote the recovery of chemically injured basal forebrain cholinergic neurons. NGF was administered on the eye of adult male mice previously treated i.c.v. with ibotenic acid to impair cholinergic pathways. Mice were tested in the passive avoidance test, and after 2 weeks of NGF administration were killed and the brains used for structural, biochemical and molecular analyses. The results showed that application of NGF on the eye surface protected choline acetyl transferase levels. These findings strengthen the hypothesis that application of NGF on the eye can represent an alternative delivery route to promote the recovery of brain cells during degeneration, including neurons involved in learning and memory activities.

Original languageEnglish
Pages (from-to)2473-2480
Number of pages8
JournalEuropean Journal of Neuroscience
Volume26
Issue number9
DOIs
Publication statusPublished - Nov 2007

Keywords

  • Behaviour
  • ChAT
  • Ibotenic acid
  • NGF
  • Passive avoidance

ASJC Scopus subject areas

  • Neuroscience(all)

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