EZH2 down-regulation exacerbates lipid accumulation and inflammation in in vitro and in vivo NAFLD

Serena Vella, Daniela Gnani, Annalisa Crudele, Sara Ceccarelli, Cristiano De Stefanis, Stefania Gaspari, Valerio Nobili, Franco Locatelli, Victor E. Marquez, Rossella Rota, Anna Alisi

Research output: Contribution to journalArticle

Abstract

Non-alcoholic fatty liver disease (NAFLD) is one of the most prevalent, chronic liver diseases, worldwide. It is a multifactorial disease caused by complex interactions between genetic, epigenetic and environmental factors. Recently, several microRNAs, some of which epigenetically regulated, have been found to be up- and/or down-regulated during NAFLD development. However, in NAFLD, the essential role of the Polycomb Group protein Enhancer of Zeste Homolog 2 (EZH2), which controls the epigenetic silencing of specific genes and/or microRNAs by trimethylating Lys27 on histone H3, still remains unknown. In this study, we demonstrate that the nuclear expression/activity of the EZH2 protein is down-regulated both in livers from NAFLD rats and in the free fatty acid-treated HepG2. The drop in EZH2 is inversely correlated with: (i) lipid accumulation; (ii) the expression of pro-inflammatory markers including TNF-α and TGF-β; and (iii) the expression of miR-200b and miR-155. Consistently, the pharmacological inhibition of EZH2 by 3-Deazaneplanocin A (DZNep) significantly reduces EZH2 expression/activity, while it increases lipid accumulation, inflammatory molecules and microRNAs. In conclusion, the results of this study suggest that the defective activity of EZH2 can enhance the NAFLD development by favouring steatosis and the de-repression of the inflammatory genes and that of specific microRNAs.

Original languageEnglish
Pages (from-to)24154-24168
Number of pages15
JournalInternational Journal of Molecular Sciences
Volume14
Issue number12
DOIs
Publication statusPublished - Dec 12 2013

Keywords

  • DZNep
  • EZH2
  • microRNAs
  • NAFLD

ASJC Scopus subject areas

  • Computer Science Applications
  • Molecular Biology
  • Catalysis
  • Inorganic Chemistry
  • Spectroscopy
  • Organic Chemistry
  • Physical and Theoretical Chemistry

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