QUANTIFICAZIONE DELLE PROSTAGLANDINE F2α, F1α, E2, E1 NEL PLASMA E NELL'EPIDERMIDE DI SOGGETTI UMANI NORMALI

S. Passi; M. Picardo; V. Nazzaro

QUANTIFICAZIONE DELLE PROSTAGLANDINE F2α, F1α, E2, E1 NEL PLASMA E NELL'EPIDERMIDE DI SOGGETTI UMANI NORMALI

Translated title of the contribution: F2α, F1α, E2, E1 Prostaglandins quantification in plasma and epidermis of normal human subjects

S. Passi, M. Picardo, V. Nazzaro

Istituto S. Maria e S. Gallicano

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

A method is described for the quantification of prostaglandins in biological samples. Standard prostaglandins F2α, F1α, E2, E1 and several catabolites were transformed in their respective para-bromophenacyl esters and then separated by reversed phase High Pressure Liquid Chromatography on 5 μm RP18 column. Simultaneous quantification of less than 20 nanograms of each prostaglandin was obtained by absorbance measurement at 254 nm. Because of the picogram levels of prostaglandins in biological samples, the radioimmunologic assay was applied to the para-bromophenacyl esters of prostaglandins separated and collected by HPLC. By this system it has been possible to measure separately plasma and epidermis levels of prostaglandins F2α, F1α, E2, E1 of 10 normal human subjects.

Translated title of the contribution	F2α, F1α, E2, E1 Prostaglandins quantification in plasma and epidermis of normal human subjects
Original language	Italian
Title of host publication	Bollettino dell Istituto Dermatologico S.Gallicano
Pages	245-256
Number of pages	12
Volume	10
Edition	2
Publication status	Published - 1980

ASJC Scopus subject areas

Medicine(all)

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Cite this

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title = "QUANTIFICAZIONE DELLE PROSTAGLANDINE F2α, F1α, E2, E1 NEL PLASMA E NELL'EPIDERMIDE DI SOGGETTI UMANI NORMALI",

abstract = "A method is described for the quantification of prostaglandins in biological samples. Standard prostaglandins F2α, F1α, E2, E1 and several catabolites were transformed in their respective para-bromophenacyl esters and then separated by reversed phase High Pressure Liquid Chromatography on 5 μm RP18 column. Simultaneous quantification of less than 20 nanograms of each prostaglandin was obtained by absorbance measurement at 254 nm. Because of the picogram levels of prostaglandins in biological samples, the radioimmunologic assay was applied to the para-bromophenacyl esters of prostaglandins separated and collected by HPLC. By this system it has been possible to measure separately plasma and epidermis levels of prostaglandins F2α, F1α, E2, E1 of 10 normal human subjects.",

author = "S. Passi and M. Picardo and V. Nazzaro",

year = "1980",

language = "Italian",

volume = "10",

pages = "245--256",

booktitle = "Bollettino dell Istituto Dermatologico S.Gallicano",

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T1 - QUANTIFICAZIONE DELLE PROSTAGLANDINE F2α, F1α, E2, E1 NEL PLASMA E NELL'EPIDERMIDE DI SOGGETTI UMANI NORMALI

AU - Passi, S.

AU - Picardo, M.

AU - Nazzaro, V.

PY - 1980

Y1 - 1980

N2 - A method is described for the quantification of prostaglandins in biological samples. Standard prostaglandins F2α, F1α, E2, E1 and several catabolites were transformed in their respective para-bromophenacyl esters and then separated by reversed phase High Pressure Liquid Chromatography on 5 μm RP18 column. Simultaneous quantification of less than 20 nanograms of each prostaglandin was obtained by absorbance measurement at 254 nm. Because of the picogram levels of prostaglandins in biological samples, the radioimmunologic assay was applied to the para-bromophenacyl esters of prostaglandins separated and collected by HPLC. By this system it has been possible to measure separately plasma and epidermis levels of prostaglandins F2α, F1α, E2, E1 of 10 normal human subjects.

AB - A method is described for the quantification of prostaglandins in biological samples. Standard prostaglandins F2α, F1α, E2, E1 and several catabolites were transformed in their respective para-bromophenacyl esters and then separated by reversed phase High Pressure Liquid Chromatography on 5 μm RP18 column. Simultaneous quantification of less than 20 nanograms of each prostaglandin was obtained by absorbance measurement at 254 nm. Because of the picogram levels of prostaglandins in biological samples, the radioimmunologic assay was applied to the para-bromophenacyl esters of prostaglandins separated and collected by HPLC. By this system it has been possible to measure separately plasma and epidermis levels of prostaglandins F2α, F1α, E2, E1 of 10 normal human subjects.

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