QUANTIFICAZIONE DELLE PROSTAGLANDINE F2α, F1α, E2, E1 NEL PLASMA E NELL'EPIDERMIDE DI SOGGETTI UMANI NORMALI

Translated title of the contribution: F2α, F1α, E2, E1 Prostaglandins quantification in plasma and epidermis of normal human subjects

S. Passi, M. Picardo, V. Nazzaro

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

A method is described for the quantification of prostaglandins in biological samples. Standard prostaglandins F2α, F1α, E2, E1 and several catabolites were transformed in their respective para-bromophenacyl esters and then separated by reversed phase High Pressure Liquid Chromatography on 5 μm RP18 column. Simultaneous quantification of less than 20 nanograms of each prostaglandin was obtained by absorbance measurement at 254 nm. Because of the picogram levels of prostaglandins in biological samples, the radioimmunologic assay was applied to the para-bromophenacyl esters of prostaglandins separated and collected by HPLC. By this system it has been possible to measure separately plasma and epidermis levels of prostaglandins F2α, F1α, E2, E1 of 10 normal human subjects.

Original languageItalian
Title of host publicationBollettino dell Istituto Dermatologico S.Gallicano
Pages245-256
Number of pages12
Volume10
Edition2
Publication statusPublished - 1980

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ASJC Scopus subject areas

  • Medicine(all)

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Passi, S., Picardo, M., & Nazzaro, V. (1980). QUANTIFICAZIONE DELLE PROSTAGLANDINE F2α, F1α, E2, E1 NEL PLASMA E NELL'EPIDERMIDE DI SOGGETTI UMANI NORMALI. In Bollettino dell Istituto Dermatologico S.Gallicano (2 ed., Vol. 10, pp. 245-256)

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