A method is described for the quantification of prostaglandins in biological samples. Standard prostaglandins F2α, F1α, E2, E1 and several catabolites were transformed in their respective para-bromophenacyl esters and then separated by reversed phase High Pressure Liquid Chromatography on 5 μm RP18 column. Simultaneous quantification of less than 20 nanograms of each prostaglandin was obtained by absorbance measurement at 254 nm. Because of the picogram levels of prostaglandins in biological samples, the radioimmunologic assay was applied to the para-bromophenacyl esters of prostaglandins separated and collected by HPLC. By this system it has been possible to measure separately plasma and epidermis levels of prostaglandins F2α, F1α, E2, E1 of 10 normal human subjects.
|Title of host publication||Bollettino dell Istituto Dermatologico S.Gallicano|
|Number of pages||12|
|Publication status||Published - 1980|
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