FACIT collagen (1α-chain) is expressed by hemocytes and epidermis during the inflammatory response of the ascidian Ciona intestinalis

Aiti Vizzini, Margherita Pergolizzi, Mirella Vazzana, Giuseppina Salerno, Caterina Di Sano, Pasquale Macaluso, Vincenzo Arizza, Daniela Parrinello, Matteo Cammarata, Nicolò Parrinello

Research output: Contribution to journalArticle

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Abstract

Based on previous cloning and sequencing study, real-time PCR and in situ hybridization assays of the inflamed body wall of LPS-injected Ciona intestinalis showed the enhanced gene expression of a collagen with FACIT structural features (Ci-type IX-Col 1α-chain). By using specific antibodies raised against an opportunely chosen Ci-type IX-Col synthetic peptide, the fibroblast property of hemocytes challenged in vitro with LPS (at 4 h) was displayed by flow cytometry, while immunocytochemistry identified hemocytes with large granules (morula cells) as collagen-producing cells. Hemocyte lysate supernatant analyzed in immunoblotting contained a 60 kDa band identifiable as 1α-chain-Ci-type IX-Col. Observations of body wall sections (immunohistochemistry method) supported the role of hemocytes and showed that epidermis expressed Ci-type IX-Col 1α-chain in the time course of the inflammatory reaction (within 24 h). Transcript and protein were mainly found in the epidermis that outlined the proximal side of the tunic matrix (at 24 h after LPS injection), in cells associated with the epidermis at 4 and 192 h. In conclusion, the C. intestinalis inflammatory response to LPS challenge appeared to be composed of a complex reaction set, and for the first time we showed in ascidians a granulation tissue with FACIT-collagen production that could participate in inflammation and wound healing. Like in vertebrates, C. intestinalis acute inflammatory reactions result in a regulated pattern of tissue repair with collagen expression during remodelling. Ci-type IX-Col could be involved in a network of non-fibril-forming collagens that participates in the organization of extracellular matrix and defense responses.

Original languageEnglish
Pages (from-to)682-692
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume32
Issue number6
DOIs
Publication statusPublished - 2008

Fingerprint

Fibril-Associated Collagens
Ciona intestinalis
Hemocytes
Urochordata
Epidermis
Collagen
Immunohistochemistry
Morula
Granulation Tissue
Immunoblotting
Wound Healing
In Situ Hybridization
Extracellular Matrix
Vertebrates
Organism Cloning
Real-Time Polymerase Chain Reaction
Flow Cytometry
Fibroblasts
Inflammation
Gene Expression

Keywords

  • Ciona intestinalis
  • Hemocytes
  • Inflammatory response
  • Type IX collagen

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

FACIT collagen (1α-chain) is expressed by hemocytes and epidermis during the inflammatory response of the ascidian Ciona intestinalis. / Vizzini, Aiti; Pergolizzi, Margherita; Vazzana, Mirella; Salerno, Giuseppina; Di Sano, Caterina; Macaluso, Pasquale; Arizza, Vincenzo; Parrinello, Daniela; Cammarata, Matteo; Parrinello, Nicolò.

In: Developmental and Comparative Immunology, Vol. 32, No. 6, 2008, p. 682-692.

Research output: Contribution to journalArticle

Vizzini, A, Pergolizzi, M, Vazzana, M, Salerno, G, Di Sano, C, Macaluso, P, Arizza, V, Parrinello, D, Cammarata, M & Parrinello, N 2008, 'FACIT collagen (1α-chain) is expressed by hemocytes and epidermis during the inflammatory response of the ascidian Ciona intestinalis', Developmental and Comparative Immunology, vol. 32, no. 6, pp. 682-692. https://doi.org/10.1016/j.dci.2007.10.006
Vizzini, Aiti ; Pergolizzi, Margherita ; Vazzana, Mirella ; Salerno, Giuseppina ; Di Sano, Caterina ; Macaluso, Pasquale ; Arizza, Vincenzo ; Parrinello, Daniela ; Cammarata, Matteo ; Parrinello, Nicolò. / FACIT collagen (1α-chain) is expressed by hemocytes and epidermis during the inflammatory response of the ascidian Ciona intestinalis. In: Developmental and Comparative Immunology. 2008 ; Vol. 32, No. 6. pp. 682-692.
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abstract = "Based on previous cloning and sequencing study, real-time PCR and in situ hybridization assays of the inflamed body wall of LPS-injected Ciona intestinalis showed the enhanced gene expression of a collagen with FACIT structural features (Ci-type IX-Col 1α-chain). By using specific antibodies raised against an opportunely chosen Ci-type IX-Col synthetic peptide, the fibroblast property of hemocytes challenged in vitro with LPS (at 4 h) was displayed by flow cytometry, while immunocytochemistry identified hemocytes with large granules (morula cells) as collagen-producing cells. Hemocyte lysate supernatant analyzed in immunoblotting contained a 60 kDa band identifiable as 1α-chain-Ci-type IX-Col. Observations of body wall sections (immunohistochemistry method) supported the role of hemocytes and showed that epidermis expressed Ci-type IX-Col 1α-chain in the time course of the inflammatory reaction (within 24 h). Transcript and protein were mainly found in the epidermis that outlined the proximal side of the tunic matrix (at 24 h after LPS injection), in cells associated with the epidermis at 4 and 192 h. In conclusion, the C. intestinalis inflammatory response to LPS challenge appeared to be composed of a complex reaction set, and for the first time we showed in ascidians a granulation tissue with FACIT-collagen production that could participate in inflammation and wound healing. Like in vertebrates, C. intestinalis acute inflammatory reactions result in a regulated pattern of tissue repair with collagen expression during remodelling. Ci-type IX-Col could be involved in a network of non-fibril-forming collagens that participates in the organization of extracellular matrix and defense responses.",
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AU - Salerno, Giuseppina

AU - Di Sano, Caterina

AU - Macaluso, Pasquale

AU - Arizza, Vincenzo

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AU - Cammarata, Matteo

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