Factors affecting lymphocyte subset reconstitution after either related or unrelated cord blood transplantation in children - A Eurocord analysis

Tim Niehues, Vanderson Rocha, Alexandra H. Filipovich, Ka Wah Chan, Raphael Porcher, Gerard Michel, Juan J. Ortega, Peter Wernet, Ulrich Göbel, Eliane Gluckman, Franco Locatelli

Research output: Contribution to journalArticle

Abstract

Immune recovery after cord blood transplantation (CBT) is of concern owing to the low number of lymphocytes transferred with the graft and their immaturity. Risk factors influencing lymphocyte subset reconstitution related to disease, patient, donor and transplant were studied in 63 children (<16 years), given either related (n = 14) or unrelated (n = 49) CBT for malignant (n = 33) or non-malignant diseases (n = 30). Only children with sustained myeloid engraftment were analysed. Absolute numbers of T (CD3+, CD4+, CD8+), B and natural killer (NK) cells were reported 2-3, 6, 9, 12 and 12-24 months after CBT. Median patient age was 4.0 years (0-15) and median follow-up was 23 months (1.7-61.0). Twenty-six patients received human leucocyte antigen (HLA)-matched CBT and 37 received HLA-mismatched CBT. The median number of nucleated cells (NCs) collected/recipient weight was 6.1 × 107/kg. In this selected population, the estimate 2 year survival was 85%. Lymphocyte reconstitution (defined as the median time to reach the normal value of age-matched healthy children) was 3, 6 and 8 months for NK, B and CD8+ cells, while it was 11.7 months for both CD3+ and CD4+ lymphocytes. In the multivariate analysis, factors favouring T-cell recovery were: related donor (P = 0.002); higher NCs/kg (P = 0.005) and recipient cytomegalovirus (CMV)-positive serology (P = 0.04). Presence of acute graft-versus-host disease (GVHD) delayed T-cell recovery (P = 0.04). To summarize, in children with sustained myeloid engraftment the concern that lymphocyte recovery after CBT could be delayed does not appear to be substantiated by our results.

Original languageEnglish
Pages (from-to)42-48
Number of pages7
JournalBritish Journal of Haematology
Volume114
Issue number1
DOIs
Publication statusPublished - 2001

Fingerprint

Lymphocyte Subsets
Fetal Blood
Transplantation
Lymphocytes
HLA Antigens
Tissue Donors
TCF Transcription Factors
Only Child
Lymphocyte Count
Graft vs Host Disease
Serology
Cytomegalovirus
Natural Killer Cells
Reference Values
Multivariate Analysis
Cell Count
T-Lymphocytes
Transplants
Weights and Measures
Survival

Keywords

  • B-cell recovery
  • Immune reconstitution
  • NK-cell recovery
  • T-cell recovery
  • Umbilical cord blood transplantation

ASJC Scopus subject areas

  • Hematology

Cite this

Factors affecting lymphocyte subset reconstitution after either related or unrelated cord blood transplantation in children - A Eurocord analysis. / Niehues, Tim; Rocha, Vanderson; Filipovich, Alexandra H.; Chan, Ka Wah; Porcher, Raphael; Michel, Gerard; Ortega, Juan J.; Wernet, Peter; Göbel, Ulrich; Gluckman, Eliane; Locatelli, Franco.

In: British Journal of Haematology, Vol. 114, No. 1, 2001, p. 42-48.

Research output: Contribution to journalArticle

Niehues, Tim ; Rocha, Vanderson ; Filipovich, Alexandra H. ; Chan, Ka Wah ; Porcher, Raphael ; Michel, Gerard ; Ortega, Juan J. ; Wernet, Peter ; Göbel, Ulrich ; Gluckman, Eliane ; Locatelli, Franco. / Factors affecting lymphocyte subset reconstitution after either related or unrelated cord blood transplantation in children - A Eurocord analysis. In: British Journal of Haematology. 2001 ; Vol. 114, No. 1. pp. 42-48.
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AU - Chan, Ka Wah

AU - Porcher, Raphael

AU - Michel, Gerard

AU - Ortega, Juan J.

AU - Wernet, Peter

AU - Göbel, Ulrich

AU - Gluckman, Eliane

AU - Locatelli, Franco

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AB - Immune recovery after cord blood transplantation (CBT) is of concern owing to the low number of lymphocytes transferred with the graft and their immaturity. Risk factors influencing lymphocyte subset reconstitution related to disease, patient, donor and transplant were studied in 63 children (<16 years), given either related (n = 14) or unrelated (n = 49) CBT for malignant (n = 33) or non-malignant diseases (n = 30). Only children with sustained myeloid engraftment were analysed. Absolute numbers of T (CD3+, CD4+, CD8+), B and natural killer (NK) cells were reported 2-3, 6, 9, 12 and 12-24 months after CBT. Median patient age was 4.0 years (0-15) and median follow-up was 23 months (1.7-61.0). Twenty-six patients received human leucocyte antigen (HLA)-matched CBT and 37 received HLA-mismatched CBT. The median number of nucleated cells (NCs) collected/recipient weight was 6.1 × 107/kg. In this selected population, the estimate 2 year survival was 85%. Lymphocyte reconstitution (defined as the median time to reach the normal value of age-matched healthy children) was 3, 6 and 8 months for NK, B and CD8+ cells, while it was 11.7 months for both CD3+ and CD4+ lymphocytes. In the multivariate analysis, factors favouring T-cell recovery were: related donor (P = 0.002); higher NCs/kg (P = 0.005) and recipient cytomegalovirus (CMV)-positive serology (P = 0.04). Presence of acute graft-versus-host disease (GVHD) delayed T-cell recovery (P = 0.04). To summarize, in children with sustained myeloid engraftment the concern that lymphocyte recovery after CBT could be delayed does not appear to be substantiated by our results.

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