Factors contributing to epidemic MRSA clones replacement in a hospital setting

Rossella Baldan, Francesca Testa, Nicola I. Lorè, Alessandra Bragonzi, Paola Cichero, Cristina Ossi, Anna Biancardi, Paola Nizzero, Matteo Moro, Daniela M. Cirillo

Research output: Contribution to journalArticle

Abstract

The mechanisms governing the epidemiology dynamics and success determinants of a specific healthcare-associated methicillin-resistant S. aureus (HA-MRSA) clone in hospital settings are still unclear. Important epidemiological changes have occurred in Europe since 2000 that have been related to the appearance of the ST22-IV clone. Between 2006 and 2010, we observed the establishment of the ST22-IV clone displacing the predominant Italian clone, ST228-I, in a large Italian university hospital. To investigate the factors associated with a successful spread of epidemic MRSA clones we studied the biofilm production, the competitive behavior in co-culture, the capacity of invasion of the A549 cells, and the susceptibility to infection in a murine model of acute pneumonia of the two major HA-MRSA clones, ST22-IV and ST228-I. We showed that persistence of ST22-IV is associated with its increased biofilm production and capacity to inhibit the growth of ST228-I in co-culture. Compared to ST228-I, ST22-IV had a significantly higher capacity to invade the A549 cells and a higher virulence in a murine model of acute lung infection causing severe inflammation and determining death in all the mice within 60 hours. On the contrary, ST228-I was associated with mice survival and clearance of the infection. ST22-IV, compared with ST228-I, caused a higher number of persistent, long lasting bacteremia. These data suggest that ST22-IV could have exploited its capacity to i) increase its biofilm production over time, ii) maintain its growth kinetics in the presence of a competitor and iii) be particularly invasive and virulent both in vitro and in vivo, to replace other well-established MRSA clones, becoming the predominant European clone.

Original languageEnglish
Article numbere43153
JournalPLoS One
Volume7
Issue number8
DOIs
Publication statusPublished - Aug 14 2012

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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    Baldan, R., Testa, F., Lorè, N. I., Bragonzi, A., Cichero, P., Ossi, C., Biancardi, A., Nizzero, P., Moro, M., & Cirillo, D. M. (2012). Factors contributing to epidemic MRSA clones replacement in a hospital setting. PLoS One, 7(8), [e43153]. https://doi.org/10.1371/journal.pone.0043153