Faf1 is expressed during neurodevelopment and is involved in Apaf1-dependent caspase-3 activation in proneural cells

D. De Zio, E. Ferraro, M. D'Amelio, V. Simoni, M. Bordi, D. Soroldoni, L. Berghella, B. I. Meyer, F. Cecconi

Research output: Contribution to journalArticle

Abstract

Fas-associated factor 1 (Faf1) has been described as a Fas-binding pro-apoptotic protein and as a component of the death-inducing signaling complex (DISC) in Fas-mediated apoptosis. Faf1 is able to potentiate Fas-induced apoptosis in several cell lines, although its specific functions are still not clear. Here we show that Faf1 is highly expressed in several areas of the developing telencephalon. Its expression pattern appears to be dynamic at different embryonic stages and to be progressively confined within limited territories. To decipher the specific role of Faf1 in developing brain, we used cDNA over-expression and mRNA down-regulation experiments to modulate Faf1 expression in telencephalic neural precursor cells, and we showed that in neural cell death Faf1 acts as a Fas-independent apoptotic enhancer. Moreover, we found that Faf1 protein level is down-regulated during apoptosis in a caspase- and Apaf1-dependent manner.

Original languageEnglish
Pages (from-to)1780-1790
Number of pages11
JournalCellular and Molecular Life Sciences
Volume65
Issue number11
DOIs
Publication statusPublished - Jun 2008

Keywords

  • Apaf1
  • Apoptosis
  • Caspase(s)
  • Cell death
  • Neurodevelopment

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

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