Failure of HIV-1 to infect human oocytes directly

Baccio Baccetti, Arrigo Benedetto, Giulia Collodel, Nicla Crisá, Antonino Di Caro, Anna Rosa Garbuglia, Paola Piomboni

Research output: Contribution to journalArticle

Abstract

In this study, the susceptibility of mature human oocytes to HIV-1 infection has been investigated. We exposed in vitro human oocytes of healthy women using inocula of cell-free HIV-1. We also tested for the presence of HIV-1-specific receptor molecules on the surface of these cells. By applying polymerase chain reaction (PCR) analysis, transmission electron microscopy (TEM), and immunocytochemistry at both light and electron microscopic levels, we did not obtain evidence for HIV DNA production nor for oocyte-associated HIV particles. Experiments of immunostaining for CD4, CCR5, and GalAAG (putative receptor for HIV in sperm), as well as reverse transcriptase (RT)- PCR for CD4, CCR5, and CXCR4, which all suggested the absence of the mentioned receptors in mature oocytes and in follicular cells. This study fills an important gap concerning the information available on the direct HIV infection of human gametes, adds to our basic understanding of HIV infection in human oocytes, provides different results from those obtained with human spermatozoa using comparable methods, and provides a basic contribution to the investigation on HIV infection in human oocytes.

Original languageEnglish
Pages (from-to)355-361
Number of pages7
JournalJournal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume21
Issue number5
Publication statusPublished - Aug 15 1999

Fingerprint

Oocytes
HIV-1
HIV Infections
Spermatozoa
HIV Receptors
HIV
Reverse Transcriptase Polymerase Chain Reaction
Transmission Electron Microscopy
Germ Cells
Immunohistochemistry
Electrons
Light
Polymerase Chain Reaction
DNA

Keywords

  • HIV-1
  • Oocytes
  • Polymerase chain reaction
  • Receptors
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Virology

Cite this

Failure of HIV-1 to infect human oocytes directly. / Baccetti, Baccio; Benedetto, Arrigo; Collodel, Giulia; Crisá, Nicla; Di Caro, Antonino; Garbuglia, Anna Rosa; Piomboni, Paola.

In: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, Vol. 21, No. 5, 15.08.1999, p. 355-361.

Research output: Contribution to journalArticle

Baccetti, B, Benedetto, A, Collodel, G, Crisá, N, Di Caro, A, Garbuglia, AR & Piomboni, P 1999, 'Failure of HIV-1 to infect human oocytes directly', Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, vol. 21, no. 5, pp. 355-361.
Baccetti, Baccio ; Benedetto, Arrigo ; Collodel, Giulia ; Crisá, Nicla ; Di Caro, Antonino ; Garbuglia, Anna Rosa ; Piomboni, Paola. / Failure of HIV-1 to infect human oocytes directly. In: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology. 1999 ; Vol. 21, No. 5. pp. 355-361.
@article{5bfb65d263ee4584b7e0d935b3233551,
title = "Failure of HIV-1 to infect human oocytes directly",
abstract = "In this study, the susceptibility of mature human oocytes to HIV-1 infection has been investigated. We exposed in vitro human oocytes of healthy women using inocula of cell-free HIV-1. We also tested for the presence of HIV-1-specific receptor molecules on the surface of these cells. By applying polymerase chain reaction (PCR) analysis, transmission electron microscopy (TEM), and immunocytochemistry at both light and electron microscopic levels, we did not obtain evidence for HIV DNA production nor for oocyte-associated HIV particles. Experiments of immunostaining for CD4, CCR5, and GalAAG (putative receptor for HIV in sperm), as well as reverse transcriptase (RT)- PCR for CD4, CCR5, and CXCR4, which all suggested the absence of the mentioned receptors in mature oocytes and in follicular cells. This study fills an important gap concerning the information available on the direct HIV infection of human gametes, adds to our basic understanding of HIV infection in human oocytes, provides different results from those obtained with human spermatozoa using comparable methods, and provides a basic contribution to the investigation on HIV infection in human oocytes.",
keywords = "HIV-1, Oocytes, Polymerase chain reaction, Receptors, Transmission electron microscopy",
author = "Baccio Baccetti and Arrigo Benedetto and Giulia Collodel and Nicla Cris{\'a} and {Di Caro}, Antonino and Garbuglia, {Anna Rosa} and Paola Piomboni",
year = "1999",
month = "8",
day = "15",
language = "English",
volume = "21",
pages = "355--361",
journal = "Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology",
issn = "1077-9450",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Failure of HIV-1 to infect human oocytes directly

AU - Baccetti, Baccio

AU - Benedetto, Arrigo

AU - Collodel, Giulia

AU - Crisá, Nicla

AU - Di Caro, Antonino

AU - Garbuglia, Anna Rosa

AU - Piomboni, Paola

PY - 1999/8/15

Y1 - 1999/8/15

N2 - In this study, the susceptibility of mature human oocytes to HIV-1 infection has been investigated. We exposed in vitro human oocytes of healthy women using inocula of cell-free HIV-1. We also tested for the presence of HIV-1-specific receptor molecules on the surface of these cells. By applying polymerase chain reaction (PCR) analysis, transmission electron microscopy (TEM), and immunocytochemistry at both light and electron microscopic levels, we did not obtain evidence for HIV DNA production nor for oocyte-associated HIV particles. Experiments of immunostaining for CD4, CCR5, and GalAAG (putative receptor for HIV in sperm), as well as reverse transcriptase (RT)- PCR for CD4, CCR5, and CXCR4, which all suggested the absence of the mentioned receptors in mature oocytes and in follicular cells. This study fills an important gap concerning the information available on the direct HIV infection of human gametes, adds to our basic understanding of HIV infection in human oocytes, provides different results from those obtained with human spermatozoa using comparable methods, and provides a basic contribution to the investigation on HIV infection in human oocytes.

AB - In this study, the susceptibility of mature human oocytes to HIV-1 infection has been investigated. We exposed in vitro human oocytes of healthy women using inocula of cell-free HIV-1. We also tested for the presence of HIV-1-specific receptor molecules on the surface of these cells. By applying polymerase chain reaction (PCR) analysis, transmission electron microscopy (TEM), and immunocytochemistry at both light and electron microscopic levels, we did not obtain evidence for HIV DNA production nor for oocyte-associated HIV particles. Experiments of immunostaining for CD4, CCR5, and GalAAG (putative receptor for HIV in sperm), as well as reverse transcriptase (RT)- PCR for CD4, CCR5, and CXCR4, which all suggested the absence of the mentioned receptors in mature oocytes and in follicular cells. This study fills an important gap concerning the information available on the direct HIV infection of human gametes, adds to our basic understanding of HIV infection in human oocytes, provides different results from those obtained with human spermatozoa using comparable methods, and provides a basic contribution to the investigation on HIV infection in human oocytes.

KW - HIV-1

KW - Oocytes

KW - Polymerase chain reaction

KW - Receptors

KW - Transmission electron microscopy

UR - http://www.scopus.com/inward/record.url?scp=0032838430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032838430&partnerID=8YFLogxK

M3 - Article

C2 - 10458615

AN - SCOPUS:0032838430

VL - 21

SP - 355

EP - 361

JO - Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology

JF - Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology

SN - 1077-9450

IS - 5

ER -