Failure to detect thyroid growth-promoting activity in immunoglobulin G of patients with endemic goiter

Paolo Vitti, Luca Chiovato, Massimo Tonacchera, Giovanna Bendinelli, Claudia Mammoli, Anna Capaccioli, Emilio Fiore, Eduardo Pretell, Aldo Pinchera

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Abstract

The [3H]thymidine incorporation assay in FRTL-5 cells was used to measure thyroid growth-stimulating antibody in the purified immunoglobulin G (IgG) fraction of patients with endemic nontoxic goiter (grade I-III) living in Italy (n = 34) or Peru (n = 37). IgG of euthyroid nongoitrous subjects living in the same endemic area (n = 25) and from an area of sufficient iodine intake were used as controls. Bovine TSH (10 mU/L) and thyroid-stimulating antibody of Graves' disease produced a significant increase in [3H]thymidine incorporation and DNA content in FRTL-5 cells. IgG from Italian or Peruvian patients with endemic goiter produced a small increase in [3H]thymidine incorporation in FRTL-5 cells (131 ± 54% and 165 ± 57%, respectively), which was indistinguishable from that obtained with IgG from normal nongoitrous subjects residing in endemic or nonendemic areas (167 ± 80% and 161 ± 36%, respectively). For comparison 18 of 25 (72%) IgG of hyperthyroid patients with Graves' disease produced clear-cut increases in [3H]thymidine incorporation (1142 ± 1065%) and DNA content (219%) in FRTL-5 cells. IgG from patients with endemic goiter, at variance with Graves' IgG, did not cause an increase in DNA in FRTL-5 cells. All Graves' IgG that stimulated [3H]thymidine incorporation in FRTL-5 cells also stimulated cAMP production in this culture system, whereas no adenylate cyclase stimulation was produced by IgG from patients with endemic goiter. The prevalence of thyroglobulin antibody and thyroperoxidase antibody in endemic goiter patients did not differ from that in control subjects residing in the same iodine-deficient area. Our data show that sera of endemic goiter patients are devoid of thyroid growth-stimulating antibody and thyroid-stimulating antibody activities. These observations argue against a direct role of thyroid autoimmunity in the development of goiter in iodine-deficient areas.

Original languageEnglish
Pages (from-to)1020-1025
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume78
Issue number5
DOIs
Publication statusPublished - May 1994

Fingerprint

Endemic Goiter
Thyroid Gland
Immunoglobulin G
Thyroid-Stimulating Immunoglobulins
Thymidine
Iodine
Graves Disease
Antibodies
DNA
growth promoting activity
Peru
Thyroglobulin
Goiter
Hyperthyroidism
Growth
Autoimmunity
Adenylyl Cyclases
Italy
Assays

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Failure to detect thyroid growth-promoting activity in immunoglobulin G of patients with endemic goiter. / Vitti, Paolo; Chiovato, Luca; Tonacchera, Massimo; Bendinelli, Giovanna; Mammoli, Claudia; Capaccioli, Anna; Fiore, Emilio; Pretell, Eduardo; Pinchera, Aldo.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 78, No. 5, 05.1994, p. 1020-1025.

Research output: Contribution to journalArticle

Vitti, P, Chiovato, L, Tonacchera, M, Bendinelli, G, Mammoli, C, Capaccioli, A, Fiore, E, Pretell, E & Pinchera, A 1994, 'Failure to detect thyroid growth-promoting activity in immunoglobulin G of patients with endemic goiter', Journal of Clinical Endocrinology and Metabolism, vol. 78, no. 5, pp. 1020-1025. https://doi.org/10.1210/jc.78.5.1020
Vitti, Paolo ; Chiovato, Luca ; Tonacchera, Massimo ; Bendinelli, Giovanna ; Mammoli, Claudia ; Capaccioli, Anna ; Fiore, Emilio ; Pretell, Eduardo ; Pinchera, Aldo. / Failure to detect thyroid growth-promoting activity in immunoglobulin G of patients with endemic goiter. In: Journal of Clinical Endocrinology and Metabolism. 1994 ; Vol. 78, No. 5. pp. 1020-1025.
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abstract = "The [3H]thymidine incorporation assay in FRTL-5 cells was used to measure thyroid growth-stimulating antibody in the purified immunoglobulin G (IgG) fraction of patients with endemic nontoxic goiter (grade I-III) living in Italy (n = 34) or Peru (n = 37). IgG of euthyroid nongoitrous subjects living in the same endemic area (n = 25) and from an area of sufficient iodine intake were used as controls. Bovine TSH (10 mU/L) and thyroid-stimulating antibody of Graves' disease produced a significant increase in [3H]thymidine incorporation and DNA content in FRTL-5 cells. IgG from Italian or Peruvian patients with endemic goiter produced a small increase in [3H]thymidine incorporation in FRTL-5 cells (131 ± 54{\%} and 165 ± 57{\%}, respectively), which was indistinguishable from that obtained with IgG from normal nongoitrous subjects residing in endemic or nonendemic areas (167 ± 80{\%} and 161 ± 36{\%}, respectively). For comparison 18 of 25 (72{\%}) IgG of hyperthyroid patients with Graves' disease produced clear-cut increases in [3H]thymidine incorporation (1142 ± 1065{\%}) and DNA content (219{\%}) in FRTL-5 cells. IgG from patients with endemic goiter, at variance with Graves' IgG, did not cause an increase in DNA in FRTL-5 cells. All Graves' IgG that stimulated [3H]thymidine incorporation in FRTL-5 cells also stimulated cAMP production in this culture system, whereas no adenylate cyclase stimulation was produced by IgG from patients with endemic goiter. The prevalence of thyroglobulin antibody and thyroperoxidase antibody in endemic goiter patients did not differ from that in control subjects residing in the same iodine-deficient area. Our data show that sera of endemic goiter patients are devoid of thyroid growth-stimulating antibody and thyroid-stimulating antibody activities. These observations argue against a direct role of thyroid autoimmunity in the development of goiter in iodine-deficient areas.",
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