False-positive apoptosis signal in mouse kidney and liver detected with TUNEL assay

K. J. Pulkkanen, M. O. Laukkanen, J. Naarala, S. Yla-Herttuala

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Apoptosis is a physiological, programmed process for the elimination of cells from living organisms. Currently, one of the most frequently used methods to detect apoptosis is TUNEL assay. It has provided valuable information about apoptosis in various tissues. However, the sensitivity and the specificity of TUNEL technique have also been criticized. We detected an intense false-positive apoptotic signal in nude and Balb/c mice kidney and liver. In kidney the signal was confined to the proximal, distal and collecting tubular cells, and in liver to hepatocytes. Both tissues appeared normal in light microscopy, and no DNA ladder formation or increase in caspase-3 enzyme activity was detected. BrdU labelling and Ki-67 immunostaining did not reveal increased cell proliferation in these tissues. On the other hand, false-positive signal was not detected in testis, spleen, pancreas or renal cell carcinoma from the same animals. Also, no false-positive signal was seen in human liver or kidney samples. Although factors known to produce false-positive staining related to sample harvesting, preparation and staining protocols were eliminated, the cause of the false- positive apoptotic signal remains unknown. We conclude that caution must be exercised when examining apoptosis in mouse tissues with TUNEL assay.

Original languageEnglish
Pages (from-to)329-333
Number of pages5
JournalApoptosis
Volume5
Issue number4
DOIs
Publication statusPublished - 2000

Fingerprint

In Situ Nick-End Labeling
Liver
Assays
Tissue
Apoptosis
Kidney
Staining and Labeling
Physiological Phenomena
Ladders
Cell proliferation
Enzyme activity
Bromodeoxyuridine
Renal Cell Carcinoma
Caspase 3
Labeling
Optical microscopy
Testis
Pancreas
Microscopy
Hepatocytes

Keywords

  • False-positive
  • Immunocytochemistry
  • Kidney
  • Liver
  • Mouse
  • TUNEL

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

False-positive apoptosis signal in mouse kidney and liver detected with TUNEL assay. / Pulkkanen, K. J.; Laukkanen, M. O.; Naarala, J.; Yla-Herttuala, S.

In: Apoptosis, Vol. 5, No. 4, 2000, p. 329-333.

Research output: Contribution to journalArticle

Pulkkanen, K. J. ; Laukkanen, M. O. ; Naarala, J. ; Yla-Herttuala, S. / False-positive apoptosis signal in mouse kidney and liver detected with TUNEL assay. In: Apoptosis. 2000 ; Vol. 5, No. 4. pp. 329-333.
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