FAS engagement drives apoptosis of enterocytes of coeliac patients

L. Maiuri, C. Ciacci, V. Raia, L. Vacca, I. Ricciardelli, F. Raimondi, S. Auricchio, S. Quaratino, M. Londei

Research output: Contribution to journalArticlepeer-review

Abstract

Background - Villus atrophy is the most distinctive sign of untreated coeliac disease (CD) and epithelial apoptosis is considered to be involved in this stage of the coeliac lesion. The extent of villus atrophy is, however, not homogeneous and patients with patchy or mild lesions have been described. Aims - To address: (a) the degree of "patchiness" in untreated CD patients; and (b) to clarify if apoptosis, and eventually which trigger drives it, causes epithelial damage. Patients - Twenty of 40 untreated, 14 treated coeliac patients, and 15 controls received five or more multiple duodenal biopsies; the remaining 20 untreated CD patients had no more than three biopsies. Methods - All biopsies were analysed to monitor the presence of a "flat" mucosa. Biopsies of 14 untreated, 10 treated coeliacs, and seven controls were cultured with or without gliadin. DNA fragmentation was studied by terminal deoxynucleotidyl transferase (TdT) mediated dUTP digoxigenin nick end labelling (TUNEL), and FAS and Ki67 expression by immunohistochemistry. Antiendomysium antibodies (EMA) were surveyed in biopsy culture supernatants. Results - A pattern of patchy duodenal lesions was observed in all untreated CD patients biopsied up to five times. High enterocyte FAS expression, and a high number of TUNEL+ and Ki67+ enterocytes were detected in areas with villus atrophy but not in those with a normal morphology (p0.01) while agonist anti-FAS monoclonal antibody increased it (p

Original languageEnglish
Pages (from-to)418-424
Number of pages7
JournalGut
Volume48
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • Apoptosis
  • Coeliac disease
  • Enterocytes
  • FAS

ASJC Scopus subject areas

  • Gastroenterology

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