Fas receptor is not present on ejaculated human sperm

S. Perticarari, G. Ricci, R. Boscolo, M. De Santis, G. Pagnini, M. Martinelli, G. Presani

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Apoptosis appears to have an essential role in the control of testis germ cell number and Fas expression has been reported in apoptotic spermatocytes and spermatids. We investigated if Fas (CD95) was present on ejaculated human sperm and any relationship between Fas on sperm and the apoptotic marker Syto16. METHODS: Semen samples from 77 male partners of infertile couples were evaluated. Each sample was analysed both before and after semen preparation by conventional microscopical procedures and by flow cytometry (FC). A multiparameter FC analysis to assess simultaneously sperm concentration, sperm viability, sperm apoptosis, CD45 positive (leukocyte) and CD95 (Fas) positive cell concentration was carried out. A further 10 samples were studied by indirect immunofluorescence to confirm results. RESULTS: The mean concentration of CD95 positive cells was very low (

Original languageEnglish
Pages (from-to)1271-1279
Number of pages9
JournalHuman Reproduction
Volume23
Issue number6
DOIs
Publication statusPublished - Jun 2008

Keywords

  • Apoptosis
  • Fas
  • Flow cytometry
  • Sperm
  • Syto 16

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynaecology
  • Reproductive Medicine

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