Abstract
Fas/Apo-1 (CD95) is an apoptosis-signaling cell surface receptor belonging to the TNF receptor family. Tumor cells resistant to Fas-mediated apoptosis have been described, but to date, the mechanisms responsible for this resistance are not well understood. We found that a series of apoptosis- resistant clones from human HUT78 lymphoma cells express a splicing variant coding for a truncated Fas molecule that lacks the intracellular death- signaling domain. The mutation responsible for the FasExo8Del expression was identified as a deletion-insertion in the intron 7/exon 8 region of the Fas gene. Moreover this mutation affects the phenotype in a dominant negative fashion, i.e., even in the presence of the normal receptor.
| Original language | English |
|---|---|
| Pages (from-to) | 13-17 |
| Number of pages | 5 |
| Journal | Journal of Immunology |
| Volume | 156 |
| Issue number | 1 |
| Publication status | Published - Jan 1 1996 |
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ASJC Scopus subject areas
- Immunology
Cite this
Fas/Apo-1 (CD95) receptor lacking the intracytoplasmic signaling domain protects tumor cells from Fas-mediated apoptosis. / Cascino, Isabella; Papoff, Giuliana; De Maria, Ruggero; Testi, Roberto; Ruberti, Giovina.
In: Journal of Immunology, Vol. 156, No. 1, 01.01.1996, p. 13-17.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fas/Apo-1 (CD95) receptor lacking the intracytoplasmic signaling domain protects tumor cells from Fas-mediated apoptosis
AU - Cascino, Isabella
AU - Papoff, Giuliana
AU - De Maria, Ruggero
AU - Testi, Roberto
AU - Ruberti, Giovina
PY - 1996/1/1
Y1 - 1996/1/1
N2 - Fas/Apo-1 (CD95) is an apoptosis-signaling cell surface receptor belonging to the TNF receptor family. Tumor cells resistant to Fas-mediated apoptosis have been described, but to date, the mechanisms responsible for this resistance are not well understood. We found that a series of apoptosis- resistant clones from human HUT78 lymphoma cells express a splicing variant coding for a truncated Fas molecule that lacks the intracellular death- signaling domain. The mutation responsible for the FasExo8Del expression was identified as a deletion-insertion in the intron 7/exon 8 region of the Fas gene. Moreover this mutation affects the phenotype in a dominant negative fashion, i.e., even in the presence of the normal receptor.
AB - Fas/Apo-1 (CD95) is an apoptosis-signaling cell surface receptor belonging to the TNF receptor family. Tumor cells resistant to Fas-mediated apoptosis have been described, but to date, the mechanisms responsible for this resistance are not well understood. We found that a series of apoptosis- resistant clones from human HUT78 lymphoma cells express a splicing variant coding for a truncated Fas molecule that lacks the intracellular death- signaling domain. The mutation responsible for the FasExo8Del expression was identified as a deletion-insertion in the intron 7/exon 8 region of the Fas gene. Moreover this mutation affects the phenotype in a dominant negative fashion, i.e., even in the presence of the normal receptor.
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UR - http://www.scopus.com/inward/citedby.url?scp=0030063193&partnerID=8YFLogxK
M3 - Article
C2 - 8598453
AN - SCOPUS:0030063193
VL - 156
SP - 13
EP - 17
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 1
ER -