TY - JOUR
T1 - Fast liquid chromatographic determination of urinary trans,trans-muconic acid
AU - Buratti, M.
AU - Fustinoni, S.
AU - Colombi, A.
PY - 1996/3/3
Y1 - 1996/3/3
N2 - trans,trans-Muconic acid (1,3-butadiene-1,4-dicarboxylic acid, MA), a minor urinary metabolite of benzene exposure, was determined, after clean-up by solid-phase anion-exchange chromatography, by reversed-phase HPLC on a C18 column (5 x 0.46 cm I.D., 3 μm particle size), using formic acid-tetrahydrofuran-water (14:17:969) as mobile phase and W detection at 263 nm. The recovery of MA from spiked urine was >95% in the 50-500 μg/l range; the quantification limit was 6 μg/l; day-to-day precision, at 300 μg/l, was C.V.=9.2%; the run time was less than 10 min. Urinary MA excretion was measured in two spot urine samples of 131 benzene environmentally exposed subjects: midday values obtained in non-smokers (mean±S.D.=77±54 μg/l, n = 82) were statistically different from those of smokers (169±85 μg/l, n = 30) (P
AB - trans,trans-Muconic acid (1,3-butadiene-1,4-dicarboxylic acid, MA), a minor urinary metabolite of benzene exposure, was determined, after clean-up by solid-phase anion-exchange chromatography, by reversed-phase HPLC on a C18 column (5 x 0.46 cm I.D., 3 μm particle size), using formic acid-tetrahydrofuran-water (14:17:969) as mobile phase and W detection at 263 nm. The recovery of MA from spiked urine was >95% in the 50-500 μg/l range; the quantification limit was 6 μg/l; day-to-day precision, at 300 μg/l, was C.V.=9.2%; the run time was less than 10 min. Urinary MA excretion was measured in two spot urine samples of 131 benzene environmentally exposed subjects: midday values obtained in non-smokers (mean±S.D.=77±54 μg/l, n = 82) were statistically different from those of smokers (169±85 μg/l, n = 30) (P
KW - Benzene
KW - Muconic acid
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U2 - 10.1016/0378-4347(95)00466-1
DO - 10.1016/0378-4347(95)00466-1
M3 - Article
C2 - 8704929
AN - SCOPUS:0029967432
VL - 677
SP - 257
EP - 263
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 1387-2273
IS - 2
ER -