TY - JOUR
T1 - Fast off-line FPSE-HPLC-PDA determination of six NSAIDs in saliva samples
AU - Tartaglia, A.
AU - Kabir, A.
AU - D'Ambrosio, F.
AU - Ramundo, P.
AU - Ulusoy, S.
AU - Ulusoy, H. I.
AU - Merone, G. M.
AU - Savini, F.
AU - D'Ovidio, C.
AU - De Grazia, U.
AU - Furton, K. G.
AU - Locatelli, M.
N1 - Funding Information:
We thank MIUR ex 60%, “G. d’Annunzio” University of Chieti-Pescara, Italy for supporting the work and TUBITAK 2219 RESEARCH SCHOLARSHIP PROGRAM for supporting the researches of Dr. Songul Ulusoy. This article is based upon work from the Sample Preparation Task Force and Network, supported by the Division of Analytical Chemistry of the European Chemical Society.
Publisher Copyright:
© 2020 Elsevier B.V.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/5/1
Y1 - 2020/5/1
N2 - A fast off-line FPSE-HPLC-PDA method has been reported that allows simultaneous clean up and determination of six non-steroidal anti-inflammatory drugs (NSAIDs) in saliva samples from healthy volunteers. Particularly, furprofen, indoprofen, ketoprofen, fenbufen, flurbiprofen, and ibuprofen were chromatographically resolved. Benzyl paraben was chosen as the internal standard (BzPB, IS). These target compounds were successfully extracted from human saliva using fabric phase sorptive extraction (FPSE) and then analysed in the liquid chromatographic system by means of a short analytical column (Symmetry C18, 75 × 4.6 mm, 3.5 µm) using acetonitrile (AcN) and phosphate buffer (PBS, 30 mM; pH = 2.5) as the mobile phases. The method, validated through the calculation of all analytical parameters in accordance of International Guidelines, was applied to real saliva sample analysis collected from informed volunteers. The proposed approach that included the use of sol–gel polytetrahydrofuran (sol–gel PTHF) sorbent immobilized on cellulose support and C18 stationary phase used in HPLC, showed high potential as a fast tool for future clinical and forensic applications. The herein reported results encourage potential future application of FPSE in the forensic field. Furthermore, the FPSE membrane was tested in dried saliva spot mode (DSS) in order to check its potential use as a sampling device, also for forensic applications.
AB - A fast off-line FPSE-HPLC-PDA method has been reported that allows simultaneous clean up and determination of six non-steroidal anti-inflammatory drugs (NSAIDs) in saliva samples from healthy volunteers. Particularly, furprofen, indoprofen, ketoprofen, fenbufen, flurbiprofen, and ibuprofen were chromatographically resolved. Benzyl paraben was chosen as the internal standard (BzPB, IS). These target compounds were successfully extracted from human saliva using fabric phase sorptive extraction (FPSE) and then analysed in the liquid chromatographic system by means of a short analytical column (Symmetry C18, 75 × 4.6 mm, 3.5 µm) using acetonitrile (AcN) and phosphate buffer (PBS, 30 mM; pH = 2.5) as the mobile phases. The method, validated through the calculation of all analytical parameters in accordance of International Guidelines, was applied to real saliva sample analysis collected from informed volunteers. The proposed approach that included the use of sol–gel polytetrahydrofuran (sol–gel PTHF) sorbent immobilized on cellulose support and C18 stationary phase used in HPLC, showed high potential as a fast tool for future clinical and forensic applications. The herein reported results encourage potential future application of FPSE in the forensic field. Furthermore, the FPSE membrane was tested in dried saliva spot mode (DSS) in order to check its potential use as a sampling device, also for forensic applications.
KW - Fabric phase sorptive extraction
KW - HPLC-PDA
KW - NSAIDs
KW - Saliva samples
KW - Validation
UR - http://www.scopus.com/inward/record.url?scp=85082808951&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85082808951&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2020.122082
DO - 10.1016/j.jchromb.2020.122082
M3 - Article
C2 - 32278291
AN - SCOPUS:85082808951
VL - 1144
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
M1 - 122082
ER -