Fast-reset of pacemaking and theta-frequency resonance patterns in cerebellar Golgi cells: Simulations of their impact in vivo

Sergio Solinas, Lia Forti, Elisabetta Cesana, Jonathan Mapelli, Erik De Schutter, Egidio D'Angelo

Research output: Contribution to journalArticlepeer-review


The Golgi cells are inhibitory interneurons of the cerebellar granular layer, which respond to afferent stimulation in vivo with a burst-pause sequence interrupting their irregular background low-frequency firing (Vos et al., 1999a. Eur. J. Neurosci. 11, 2621-2634). However, Golgi cells in vitro are regular pacemakers (Forti et al., 2006. J. Physiol. 574, 711-729), raising the question how their ionic mechanisms could impact on responses during physiological activity. Using patch-clamp recordings in cerebellar slices we show that the pacemaker cycle can be suddenly reset by spikes, making the cell highly sensitive to input variations. Moreover, the neuron resonates around the pacemaker frequency, making it specifically sensitive to patterned stimulation in the theta-frequency band. Computational analysis based on a model developed to reproduce Golgi cell pacemaking (Solinas et al., 2007 Front. Cell. Neurosci., 1:2) predicted that phase-reset required spike-triggered activation of SK channels and that resonance was sustained by a slow voltage-dependent potassium current and amplified by a persistent sodium current. Adding balanced synaptic noise to mimic the irregular discharge observed in vivo, we found that pacemaking converts into spontaneous irregular discharge, that phase-reset plays an important role in generating the burst-pause pattern evoked by sensory stimulation, and that repetitive stimulation at theta-frequency enhances the time-precision of spike coding in the burst. These results suggest that Golgi cell intrinsic properties exert a profound impact on time-dependent signal processing in the cerebellar granular layer.

Original languageEnglish
Article number4
JournalFrontiers in Cellular Neuroscience
Issue numberDEC
Publication statusPublished - Dec 30 2007


  • Cerebellum
  • Golgi cell
  • Granular layer
  • Modeling
  • Phase-reset
  • Resonance

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience


Dive into the research topics of 'Fast-reset of pacemaking and theta-frequency resonance patterns in cerebellar Golgi cells: Simulations of their impact in vivo'. Together they form a unique fingerprint.

Cite this