TY - JOUR
T1 - Fast, simple and cost-effective determination of thiopental in human plasma by a new HPLC technique
AU - Coppa, Gilberto
AU - Testa, Roberto
AU - Gambini, Anna Margarucci
AU - Testa, Ivano
AU - Tocchini, Massimo
AU - Bonfigli, Anna Rita
PY - 2001
Y1 - 2001
N2 - Background: Thiopental is an anaesthetic drug that is largely used in both short-term and long-term infusion. After long-term infusion of thiopental, non-linear and inter-individual-dependent pharmacokinetics occur because of the saturation and/or induction of the metabolism. Clinical monitoring is important so that therapeutic adjustments can be made in many of the different pharmacological treatments, especially when long-term infusion is required. We describe a new, rapid HPLC method for the determination of plasma thiopental. Methods: Sample preparation involved precipitation of plasma proteins using a mixture of methanol, zinc sulfate and ethylene glycol, and containing the internal standard 5-ethyl-5-p-tolyl-barbituric acid. After adding trichloroacetic acid, the sample was centrifuged and the supernatant was injected into a C18 reversed-phase column. The mobile phase used was water-methanol-acetonitrile (50:40:10, v/v). The eluent was monitored at 290 nm. Results: The calibration curve was linear from 0.2 to 100 μg/mL. Precision, calculated as the coefficient of variation (%), was in the range of 3.62-0.70% for the within-day assay and 5.77-1.51% for the between-day assay. The absolute recoveries obtained from supplemented samples were never less than 100%. Conclusions: This technique shows good reliability and seems to be suitable for a very fast and simple therapeutic monitoring of plasma thiopental.
AB - Background: Thiopental is an anaesthetic drug that is largely used in both short-term and long-term infusion. After long-term infusion of thiopental, non-linear and inter-individual-dependent pharmacokinetics occur because of the saturation and/or induction of the metabolism. Clinical monitoring is important so that therapeutic adjustments can be made in many of the different pharmacological treatments, especially when long-term infusion is required. We describe a new, rapid HPLC method for the determination of plasma thiopental. Methods: Sample preparation involved precipitation of plasma proteins using a mixture of methanol, zinc sulfate and ethylene glycol, and containing the internal standard 5-ethyl-5-p-tolyl-barbituric acid. After adding trichloroacetic acid, the sample was centrifuged and the supernatant was injected into a C18 reversed-phase column. The mobile phase used was water-methanol-acetonitrile (50:40:10, v/v). The eluent was monitored at 290 nm. Results: The calibration curve was linear from 0.2 to 100 μg/mL. Precision, calculated as the coefficient of variation (%), was in the range of 3.62-0.70% for the within-day assay and 5.77-1.51% for the between-day assay. The absolute recoveries obtained from supplemented samples were never less than 100%. Conclusions: This technique shows good reliability and seems to be suitable for a very fast and simple therapeutic monitoring of plasma thiopental.
KW - Anaesthesia
KW - Barbiturate
KW - HPLC
KW - Thiopental
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U2 - 10.1016/S0009-8981(00)00417-4
DO - 10.1016/S0009-8981(00)00417-4
M3 - Article
C2 - 11249921
AN - SCOPUS:0035105145
VL - 305
SP - 41
EP - 45
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
IS - 1-2
ER -