Fast transcription rates of RNA polymerase II in human cells

Paolo Maiuri, Anna Knezevich, Alex De Marco, Davide Mazza, Anna Kula, Jim G. McNally, Alessandro Marcello

Research output: Contribution to journalArticle

Abstract

Averaged estimates of RNA polymerase II (RNAPII) elongation rates in mammalian cells have been shown to range between 1.3 and 4.3 kb min -1. In this work, nascent RNAs from an integrated human immunodeficiency virus type 1-derived vector were detectable at the single living cell level by fluorescent RNA tagging. At steady state, a constant number of RNAs was measured corresponding to a minimal density of polymerases with negligible fluctuations over time. Recovery of fluorescence after photobleaching was complete within seconds, indicating a high rate of RNA biogenesis. The calculated transcription rate above 50 kb min -1 points towards a wide dynamic range of RNAPII velocities in living cells.

Original languageEnglish
Pages (from-to)1280-1285
Number of pages6
JournalEMBO Reports
Volume12
Issue number12
DOIs
Publication statusPublished - Dec 2011

Fingerprint

RNA Polymerase II
Transcription
Cells
RNA
Fluorescence Recovery After Photobleaching
Photobleaching
Viruses
HIV-1
Elongation
Fluorescence
Recovery

Keywords

  • FRAP
  • HIV
  • live imaging
  • RNA polymerase II
  • transcription

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry

Cite this

Maiuri, P., Knezevich, A., De Marco, A., Mazza, D., Kula, A., McNally, J. G., & Marcello, A. (2011). Fast transcription rates of RNA polymerase II in human cells. EMBO Reports, 12(12), 1280-1285. https://doi.org/10.1038/embor.2011.196

Fast transcription rates of RNA polymerase II in human cells. / Maiuri, Paolo; Knezevich, Anna; De Marco, Alex; Mazza, Davide; Kula, Anna; McNally, Jim G.; Marcello, Alessandro.

In: EMBO Reports, Vol. 12, No. 12, 12.2011, p. 1280-1285.

Research output: Contribution to journalArticle

Maiuri, P, Knezevich, A, De Marco, A, Mazza, D, Kula, A, McNally, JG & Marcello, A 2011, 'Fast transcription rates of RNA polymerase II in human cells', EMBO Reports, vol. 12, no. 12, pp. 1280-1285. https://doi.org/10.1038/embor.2011.196
Maiuri P, Knezevich A, De Marco A, Mazza D, Kula A, McNally JG et al. Fast transcription rates of RNA polymerase II in human cells. EMBO Reports. 2011 Dec;12(12):1280-1285. https://doi.org/10.1038/embor.2011.196
Maiuri, Paolo ; Knezevich, Anna ; De Marco, Alex ; Mazza, Davide ; Kula, Anna ; McNally, Jim G. ; Marcello, Alessandro. / Fast transcription rates of RNA polymerase II in human cells. In: EMBO Reports. 2011 ; Vol. 12, No. 12. pp. 1280-1285.
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