TY - JOUR
T1 - Fatal outbreak in tonkean macaques caused by possibly novel orthopoxvirus, Italy, January 2015
AU - Cardeti, Giusy
AU - Gruber, Cesare Ernesto Maria
AU - Eleni, Claudia
AU - Carletti, Fabrizio
AU - Castilletti, Concetta
AU - Manna, Giuseppe
AU - Rosone, Francesca
AU - Giombini, Emanuela
AU - Selleri, Marina
AU - Lapa, Daniele
AU - Puro, Vincenzo
AU - Di Caro, Antonino
AU - Lorenzetti, Raniero
AU - Scicluna, Maria Teresa
AU - Grifoni, Goffredo
AU - Rizzoli, Annapaola
AU - Tagliapietra, Valentina
AU - De Marco, Lorenzo
AU - Capobianchi, Maria Rosaria
AU - Autorino, Gian Luca
PY - 2017/12/1
Y1 - 2017/12/1
N2 - In January 2015, during a 3-week period, 12 captive Tonkean macacques at a sanctuary in Italy died. An orthopoxvirus infection was suspected because of negative-staining electron microscopy results. The diagnosis was confirmed by histology, virus isolation, and molecular analysis performed on different organs from all animals. An epidemiologic investigation was unable to define the infection source in the surrounding area. Trapped rodents were negative by virologic testing, but specific IgG was detected in 27.27% of small rodents and 14.28% of rats. An attenuated live vaccine was administered to the susceptible monkey population, and no adverse reactions were observed; a detectable humoral immune response was induced in most of the vaccinated animals. We performed molecular characterization of the orthopoxvirus isolate by next-generation sequencing. According to the phylogenetic analysis of the 9 conserved genes, the virus could be part of a novel clade, lying between cowpox and ectromelia viruses.
AB - In January 2015, during a 3-week period, 12 captive Tonkean macacques at a sanctuary in Italy died. An orthopoxvirus infection was suspected because of negative-staining electron microscopy results. The diagnosis was confirmed by histology, virus isolation, and molecular analysis performed on different organs from all animals. An epidemiologic investigation was unable to define the infection source in the surrounding area. Trapped rodents were negative by virologic testing, but specific IgG was detected in 27.27% of small rodents and 14.28% of rats. An attenuated live vaccine was administered to the susceptible monkey population, and no adverse reactions were observed; a detectable humoral immune response was induced in most of the vaccinated animals. We performed molecular characterization of the orthopoxvirus isolate by next-generation sequencing. According to the phylogenetic analysis of the 9 conserved genes, the virus could be part of a novel clade, lying between cowpox and ectromelia viruses.
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U2 - 10.3201/eid2312.162098
DO - 10.3201/eid2312.162098
M3 - Article
AN - SCOPUS:85035008559
VL - 23
SP - 1941
EP - 1949
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
SN - 1080-6040
IS - 12
ER -