Purpose. In this study, fatty acid profiles of red blood cells have been determined m patients affected by retinal degeneration. All fatty acids (C>14), have been documented. Abnormalities in their distribution couid be correlated with pathoaenesis of disease. Methods. Two studies were done at the same time. The first including 11 patients with retinitis pigmentosa (RP) and 14 controls. The second, 9 patients with age related macular deeeneration(AMD) and 11 controls. AU patients have been investigated and electrophysiological responses were measured (ERG and VHP's). Red blood cells were separated by centri&galion from a fasted blood samples (10 ml). Transesterified fatty acids were quantified using capillary column gas chromatography (Supelco, Bellefonte. PA). The Meat analysis was used to compare fatty acid content in patients versus controls. Values of P<0.05 were selected as the limit of significance. Results. Patients with RP had significantly lower mean plasma percentages of the £2-3 fatty acids: C18:3t23, C20:5£23, C22:6Q3 and two saturated acids: 14:0. C20:0. The level of C24:l was on the contrary increased in the patient group. Total £23 fatty acids were significantly lower in patients with age related macular degeneration. Conclusions. Several investigators have observed lipid abnormalities in various form of RP. but there are not studies about macular degeneration. Similar alterations in both diseases were found Since high levels of £23 fatty acids (30-40% of total fatty acids) are observed in retinal tissue, low sistemic levels suggest a correlation with the pathogenesis of disease,.
|Journal||Investigative Ophthalmology and Visual Science|
|Publication status||Published - 1997|
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