Favism: A hemolytic disease associated with increased superoxide dismutase and decreased glutathione peroxidase activities in red blood cells

I. Mavelli, M. R. Ciriolo, L. Rossi, T. Meloni, G. Forteleoni, A. De Flora, U. Benatti, A. Morelli, G. Rotilio

Research output: Contribution to journalArticle

Abstract

Red blood cells of favism patients with acute hemolytic crisis have markedly more superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1) and less glutathione peroxidase (glutathione:hydrogenperoxide oxidoreductase, EC 1.11.1.9) than either normal controls, glucose-6-phosphate dehydrogenase-deficient subjects or favism patients outside hemolytic crisis. This altered value of the two enzyme activities is not due to increased reticulocyte content of blood. The electrophoretic triplet pattern of superoxide dismutase is also changed, with significant increase of the most positively charged band. Similar modifications of the two enzyme activities are observed after treatment of normal red blood cells with high concentrations of divicine and ascorbate, which are redox compounds that are contained in fava seeds. This treatment produces no hemolysis, but leads to hemolysis if th) treated cells are resuspended in the homologous plasma. These results suggest a possible role of active oxygen species in the development of favism.

Original languageEnglish
Pages (from-to)13-18
Number of pages6
JournalEuropean Journal of Biochemistry
Volume139
Issue number1
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Biochemistry

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    Mavelli, I., Ciriolo, M. R., Rossi, L., Meloni, T., Forteleoni, G., De Flora, A., Benatti, U., Morelli, A., & Rotilio, G. (1984). Favism: A hemolytic disease associated with increased superoxide dismutase and decreased glutathione peroxidase activities in red blood cells. European Journal of Biochemistry, 139(1), 13-18.