Fc-receptors for IgG and IgM immunoglobulins on human T lymphocytes: Mode of re-expression after proteolysis or interaction with immune complexes

M. C. Mingari, L. Moretta, A. Moretta, M. Ferrarini, J. L. Preud'homme

Research output: Contribution to journalArticle

Abstract

The susceptibility to proteolysis and the mode of re-expression of receptors for IgM or IgG present on two different subpopulations of human T lymphocytes (T.(M) and T.(G) cells, respectively) have been investigated. The IgM receptor was highly susceptible to both trypsin and pronase, whereas the IgG receptor was resistant to trypsin and sensistive only to high concentrations of pronase. The receptors have been removed by treating purified human T cells with pronase and their reappearance on the cell surface has been followed in vitro. The IgM receptors on the cell surface were detectable within 2 hr and the resynthesis was completed in 6 hr. IgG receptors were detectable in 4 to 6 hr and the resynthesis completed within 12 hr. When protein synthesis was inhibited by culturing the cells in the presence of cycloheximide for up to 12 hr, only the IgM receptor (which had a higher turnover rate) failed to be expressed. Whereas interaction of IgG immune complex with the IgG receptors was previously shown to induce a modulation of the receptors, contact with antigen-IgM antibody complexes did not alter the mode of expression of IgM receptors.

Original languageEnglish
Pages (from-to)767-770
Number of pages4
JournalJournal of Immunology
Volume121
Issue number2
Publication statusPublished - 1978

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Antigen-Antibody Complex
Proteolysis
Immunoglobulin M
Immunoglobulins
Immunoglobulin G
T-Lymphocytes
IgG Receptors
Pronase
Trypsin
Gastrin-Secreting Cells
Cell Surface Receptors
Cycloheximide
immunoglobulin M receptor
Proteins

ASJC Scopus subject areas

  • Immunology

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Fc-receptors for IgG and IgM immunoglobulins on human T lymphocytes : Mode of re-expression after proteolysis or interaction with immune complexes. / Mingari, M. C.; Moretta, L.; Moretta, A.; Ferrarini, M.; Preud'homme, J. L.

In: Journal of Immunology, Vol. 121, No. 2, 1978, p. 767-770.

Research output: Contribution to journalArticle

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