Feasibility of circulating miRNA microarray analysis from archival plasma samples

Maurizio Callari; Paola Tiberio; Loris De Cecco; Elena Cavadini; Matteo Dugo; Chiara Ghimenti; Maria Grazia Daidone; Silvana Canevari; Valentina Appierto

doi:10.1016/j.ab.2013.03.002

Feasibility of circulating miRNA microarray analysis from archival plasma samples

Maurizio Callari, Paola Tiberio, Loris De Cecco, Elena Cavadini, Matteo Dugo, Chiara Ghimenti, Maria Grazia Daidone, Silvana Canevari, Valentina Appierto

Fondazione IRCCS Istituto Nazionale dei Tumori

Research output: Contribution to journal › Article › peer-review

Abstract

MicroRNAs have been found to be deregulated in several diseases and, due to their high stability in body fluids, represent promising noninvasively detectable biomarkers. However, numerous technical variables can affect accurate measurement of circulating miRNAs. Using a microarray-based method we assessed the: (i) adequate intra- and inter-array reproducibility of miRNA profiling; (ii) feasibility of using archival plasma samples stored for an extended period of time and available in limited amounts; (iii) good correlation between different batches; and (iv) time-dependent increase of background signals close to the chip expiration date.

Original language	English
Pages (from-to)	123-125
Number of pages	3
Journal	Analytical Biochemistry
Volume	437
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2013.03.002
Publication status	Published - Jun 15 2013

Keywords

Agilent
Blood
Circulating miRNA
Microarray
miRNA
Plasma

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology
Cell Biology

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Feasibility of circulating miRNA microarray analysis from archival plasma samples. / Callari, Maurizio; Tiberio, Paola; De Cecco, Loris; Cavadini, Elena; Dugo, Matteo; Ghimenti, Chiara; Daidone, Maria Grazia; Canevari, Silvana; Appierto, Valentina.

In: Analytical Biochemistry, Vol. 437, No. 2, 15.06.2013, p. 123-125.

Research output: Contribution to journal › Article › peer-review

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