Feasibility of circulating miRNA microarray analysis from archival plasma samples

Maurizio Callari, Paola Tiberio, Loris De Cecco, Elena Cavadini, Matteo Dugo, Chiara Ghimenti, Maria Grazia Daidone, Silvana Canevari, Valentina Appierto

Research output: Contribution to journalArticlepeer-review

Abstract

MicroRNAs have been found to be deregulated in several diseases and, due to their high stability in body fluids, represent promising noninvasively detectable biomarkers. However, numerous technical variables can affect accurate measurement of circulating miRNAs. Using a microarray-based method we assessed the: (i) adequate intra- and inter-array reproducibility of miRNA profiling; (ii) feasibility of using archival plasma samples stored for an extended period of time and available in limited amounts; (iii) good correlation between different batches; and (iv) time-dependent increase of background signals close to the chip expiration date.

Original languageEnglish
Pages (from-to)123-125
Number of pages3
JournalAnalytical Biochemistry
Volume437
Issue number2
DOIs
Publication statusPublished - Jun 15 2013

Keywords

  • Agilent
  • Blood
  • Circulating miRNA
  • Microarray
  • miRNA
  • Plasma

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

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