Abstract
The aim of this work was to study the feasibility of the Food and Agriculture Organization (FAO) microsatellite panel developed for chickens to assess genetic variability in turkeys. Genomic DNA was extracted from a total of 37 blood samples collected from turkey of different breeds [15 Brianzolo (BR); 12 Colli Euganei (EU); 10 Nero d’Italia (NI)], and all 31 chicken microsatellite markers recommended by the FAO were tested. The results show that 22 chicken markers out of 31 suggested by FAO guidelines can be applied to turkey populations. In particular, the multiplex groups confirmed in the turkey were the Multiplex Master Mix 1 (ADL0268, ADL0278, LEI0094, MCW0216, MCW0248) and the Master Mix 2 (MCW0034, MCW0069, MCW0081, MCW0222, MCW0295), whereas 13 microsatellites were amplified only under single polymerase chain reaction (PCR) conditions. No PCR products were obtained for 9 markers (LEI0166, MCW0020, MCW0078, MCW0080, MCW0104, MCW0123, MCW0248, MCW0284 and MCW0330), which is 29% of the total markers used. A panel of 22 markers was used to assess genetic diversity in three turkey breeds and a total number of 63 alleles were found. Observed (Ho) and expected (He) heterozygosity and polymorphism information content (PIC) values for each microsatellite and the relative mean values were also calculated. The mean values were 0.210, 0.250, 0.203 for Ho; 0.301, 0.348, 0.228 for He; and 0.265, 0.313, 0.199 for PIC in NI, BR and EU, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 887-890 |
| Number of pages | 4 |
| Journal | Italian Journal of Animal Science |
| Volume | 13 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Dec 23 2014 |
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Keywords
- Biodiversity
- Breed conservation
- Genetic variability
- Microsatellite
- Turkey
ASJC Scopus subject areas
- Animal Science and Zoology
Cite this
Feasibility study on the FAO chicken microsatellite panel to assess genetic variability in the Turkey (Meleagris gallopavo). / Colombo, Elena; Strillacci, Maria G.; Cozzi, Maria C.; Madeddu, Manuela; Mangiagalli, Maria G.; Mosca, Fabio; Zaniboni, Luisa; Bagnato, Alessandro; Cerolini, Silvia.
In: Italian Journal of Animal Science, Vol. 13, No. 4, 23.12.2014, p. 887-890.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Feasibility study on the FAO chicken microsatellite panel to assess genetic variability in the Turkey (Meleagris gallopavo)
AU - Colombo, Elena
AU - Strillacci, Maria G.
AU - Cozzi, Maria C.
AU - Madeddu, Manuela
AU - Mangiagalli, Maria G.
AU - Mosca, Fabio
AU - Zaniboni, Luisa
AU - Bagnato, Alessandro
AU - Cerolini, Silvia
PY - 2014/12/23
Y1 - 2014/12/23
N2 - The aim of this work was to study the feasibility of the Food and Agriculture Organization (FAO) microsatellite panel developed for chickens to assess genetic variability in turkeys. Genomic DNA was extracted from a total of 37 blood samples collected from turkey of different breeds [15 Brianzolo (BR); 12 Colli Euganei (EU); 10 Nero d’Italia (NI)], and all 31 chicken microsatellite markers recommended by the FAO were tested. The results show that 22 chicken markers out of 31 suggested by FAO guidelines can be applied to turkey populations. In particular, the multiplex groups confirmed in the turkey were the Multiplex Master Mix 1 (ADL0268, ADL0278, LEI0094, MCW0216, MCW0248) and the Master Mix 2 (MCW0034, MCW0069, MCW0081, MCW0222, MCW0295), whereas 13 microsatellites were amplified only under single polymerase chain reaction (PCR) conditions. No PCR products were obtained for 9 markers (LEI0166, MCW0020, MCW0078, MCW0080, MCW0104, MCW0123, MCW0248, MCW0284 and MCW0330), which is 29% of the total markers used. A panel of 22 markers was used to assess genetic diversity in three turkey breeds and a total number of 63 alleles were found. Observed (Ho) and expected (He) heterozygosity and polymorphism information content (PIC) values for each microsatellite and the relative mean values were also calculated. The mean values were 0.210, 0.250, 0.203 for Ho; 0.301, 0.348, 0.228 for He; and 0.265, 0.313, 0.199 for PIC in NI, BR and EU, respectively.
AB - The aim of this work was to study the feasibility of the Food and Agriculture Organization (FAO) microsatellite panel developed for chickens to assess genetic variability in turkeys. Genomic DNA was extracted from a total of 37 blood samples collected from turkey of different breeds [15 Brianzolo (BR); 12 Colli Euganei (EU); 10 Nero d’Italia (NI)], and all 31 chicken microsatellite markers recommended by the FAO were tested. The results show that 22 chicken markers out of 31 suggested by FAO guidelines can be applied to turkey populations. In particular, the multiplex groups confirmed in the turkey were the Multiplex Master Mix 1 (ADL0268, ADL0278, LEI0094, MCW0216, MCW0248) and the Master Mix 2 (MCW0034, MCW0069, MCW0081, MCW0222, MCW0295), whereas 13 microsatellites were amplified only under single polymerase chain reaction (PCR) conditions. No PCR products were obtained for 9 markers (LEI0166, MCW0020, MCW0078, MCW0080, MCW0104, MCW0123, MCW0248, MCW0284 and MCW0330), which is 29% of the total markers used. A panel of 22 markers was used to assess genetic diversity in three turkey breeds and a total number of 63 alleles were found. Observed (Ho) and expected (He) heterozygosity and polymorphism information content (PIC) values for each microsatellite and the relative mean values were also calculated. The mean values were 0.210, 0.250, 0.203 for Ho; 0.301, 0.348, 0.228 for He; and 0.265, 0.313, 0.199 for PIC in NI, BR and EU, respectively.
KW - Biodiversity
KW - Breed conservation
KW - Genetic variability
KW - Microsatellite
KW - Turkey
UR - http://www.scopus.com/inward/record.url?scp=84919949014&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84919949014&partnerID=8YFLogxK
U2 - 10.4081/ijas.2014.3334
DO - 10.4081/ijas.2014.3334
M3 - Article
AN - SCOPUS:84919949014
VL - 13
SP - 887
EP - 890
JO - Italian Journal of Animal Science
JF - Italian Journal of Animal Science
SN - 1594-4077
IS - 4
ER -