Feasibility study on the FAO chicken microsatellite panel to assess genetic variability in the Turkey (Meleagris gallopavo)

Elena Colombo, Maria G. Strillacci, Maria C. Cozzi, Manuela Madeddu, Maria G. Mangiagalli, Fabio Mosca, Luisa Zaniboni, Alessandro Bagnato, Silvia Cerolini

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this work was to study the feasibility of the Food and Agriculture Organization (FAO) microsatellite panel developed for chickens to assess genetic variability in turkeys. Genomic DNA was extracted from a total of 37 blood samples collected from turkey of different breeds [15 Brianzolo (BR); 12 Colli Euganei (EU); 10 Nero d’Italia (NI)], and all 31 chicken microsatellite markers recommended by the FAO were tested. The results show that 22 chicken markers out of 31 suggested by FAO guidelines can be applied to turkey populations. In particular, the multiplex groups confirmed in the turkey were the Multiplex Master Mix 1 (ADL0268, ADL0278, LEI0094, MCW0216, MCW0248) and the Master Mix 2 (MCW0034, MCW0069, MCW0081, MCW0222, MCW0295), whereas 13 microsatellites were amplified only under single polymerase chain reaction (PCR) conditions. No PCR products were obtained for 9 markers (LEI0166, MCW0020, MCW0078, MCW0080, MCW0104, MCW0123, MCW0248, MCW0284 and MCW0330), which is 29% of the total markers used. A panel of 22 markers was used to assess genetic diversity in three turkey breeds and a total number of 63 alleles were found. Observed (Ho) and expected (He) heterozygosity and polymorphism information content (PIC) values for each microsatellite and the relative mean values were also calculated. The mean values were 0.210, 0.250, 0.203 for Ho; 0.301, 0.348, 0.228 for He; and 0.265, 0.313, 0.199 for PIC in NI, BR and EU, respectively.

Original languageEnglish
Pages (from-to)887-890
Number of pages4
JournalItalian Journal of Animal Science
Volume13
Issue number4
DOIs
Publication statusPublished - Dec 23 2014

Keywords

  • Biodiversity
  • Breed conservation
  • Genetic variability
  • Microsatellite
  • Turkey

ASJC Scopus subject areas

  • Animal Science and Zoology

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