Features of ceruloplasmin in the cerebrospinal fluid of Alzheimer's disease patients

Concetta R. Capo, Mario Arciello, Rosanna Squitti, Emanuele Cassetta, Paolo Maria Rossini, Lilia Calabrese, Luisa Rossi

Research output: Contribution to journalArticle

Abstract

The level of the apo-form of the copper enzyme ceruloplasmin (CP) is an established peripheral marker in diseases associated with copper imbalance. In view of the proposal that disturbances of copper homeostasis may contribute to neurodegeneration associated with Alzheimer's disease (AD), the present work investigates, by Western blot and non-reducing SDS-PAGE followed by activity staining, the features of CP protein, and the copper/CP relationship in cerebrospinal fluid (CSF) and serum of AD patients. Results show that only a fraction of total copper is associated with CP in the CSF, at variance with serum, both in affected and in healthy individuals. Furthermore, a conspicuous amount of apo-ceruloplasmin and a decrease of CP oxidase activity characterize the CSF of the affected individuals, and confirm that an impairment of copper metabolism occurs in their central nervous system. In the CSF of AD patients the decrease of active CP, associated with the increase in the pool of copper not sequestered by this protein, may play a role in the neurodegenerative process.

Original languageEnglish
Pages (from-to)367-372
Number of pages6
JournalBioMetals
Volume21
Issue number3
DOIs
Publication statusPublished - Jun 2008

Keywords

  • Alzheimer's disease
  • Cerebrospinal fluid
  • Ceruloplasmin
  • Copper
  • Serum

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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    Capo, C. R., Arciello, M., Squitti, R., Cassetta, E., Rossini, P. M., Calabrese, L., & Rossi, L. (2008). Features of ceruloplasmin in the cerebrospinal fluid of Alzheimer's disease patients. BioMetals, 21(3), 367-372. https://doi.org/10.1007/s10534-007-9125-4