TY - JOUR
T1 - Features of peripheral CD8+CD57+ lymphocytes in patients with autoimmune hemolytic anemia
AU - Fatone, Maria Celeste
AU - Pavone, Fabio
AU - Lauletta, Gianfranco
AU - Russi, Sabino
PY - 2018/5/19
Y1 - 2018/5/19
N2 - Autoimmune hemolytic anemia (AIHA) is an acquired condition characterized by the presence of autoantibodies recognizing erythrocyte-related antigens. Several components of the immune system are involved in disease pathogenesis. Among them, as for other autoimmune disorders, a role for specific CD8+CD57+ regulatory cells subset could be hypothesized. We evaluated this lymphocyte subset by flow cytometry in 18 AIHA patients randomly selected in a retrospective population of 29 cases. Secondary forms were observed in 65.5% of cases, whereas frequencies of warm, cold, mixed, and atypical forms were similar. Cold agglutinins and cryoglobulins tested positive in 44.8% and 10.3% of cases, respectively. These patients exhibited a higher frequency of peripheral vascular symptoms (odds ratio = 8.2, p =.04) and complement consumption (odds ratio = 7.2, p =.02). Frequency of CD8+CD57+ cells resulted significantly higher in AIHA patients than in control group (17.0 ± 15.8% vs 8.2 ± 5.0%, p =.04). Regardless of therapeutic schedule, patients with partial or no response to therapy (8/18) showed higher frequencies of CD8+CD57+ cells as compared with controls (23.6 ± 21.3% vs 8.9 ± 4.9%, p =.01), whereas 10/18 complete responders (CR) showed lower levels of CD8+CD57+ cells (11.7 ± 6.9%, p =.11). CR and controls showed similar values (p =.24). This study suggests that monitoring this lymphocyte subset before and after treatment administration might have a prognostic value. Moreover, CD8+CD57+ cells may represent a possible therapeutic target to restore the normal balance between lymphocyte populations.
AB - Autoimmune hemolytic anemia (AIHA) is an acquired condition characterized by the presence of autoantibodies recognizing erythrocyte-related antigens. Several components of the immune system are involved in disease pathogenesis. Among them, as for other autoimmune disorders, a role for specific CD8+CD57+ regulatory cells subset could be hypothesized. We evaluated this lymphocyte subset by flow cytometry in 18 AIHA patients randomly selected in a retrospective population of 29 cases. Secondary forms were observed in 65.5% of cases, whereas frequencies of warm, cold, mixed, and atypical forms were similar. Cold agglutinins and cryoglobulins tested positive in 44.8% and 10.3% of cases, respectively. These patients exhibited a higher frequency of peripheral vascular symptoms (odds ratio = 8.2, p =.04) and complement consumption (odds ratio = 7.2, p =.02). Frequency of CD8+CD57+ cells resulted significantly higher in AIHA patients than in control group (17.0 ± 15.8% vs 8.2 ± 5.0%, p =.04). Regardless of therapeutic schedule, patients with partial or no response to therapy (8/18) showed higher frequencies of CD8+CD57+ cells as compared with controls (23.6 ± 21.3% vs 8.9 ± 4.9%, p =.01), whereas 10/18 complete responders (CR) showed lower levels of CD8+CD57+ cells (11.7 ± 6.9%, p =.11). CR and controls showed similar values (p =.24). This study suggests that monitoring this lymphocyte subset before and after treatment administration might have a prognostic value. Moreover, CD8+CD57+ cells may represent a possible therapeutic target to restore the normal balance between lymphocyte populations.
KW - autoimmune disease
KW - Autoimmune hemolytic anemia
KW - CD8CD57 cells
KW - flow cytometry
KW - immune senescence
UR - http://www.scopus.com/inward/record.url?scp=85047834289&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85047834289&partnerID=8YFLogxK
U2 - 10.1080/08916934.2018.1477132
DO - 10.1080/08916934.2018.1477132
M3 - Article
AN - SCOPUS:85047834289
VL - 51
SP - 166
EP - 174
JO - Autoimmunity
JF - Autoimmunity
SN - 0891-6934
IS - 4
ER -