FERM protein EPB41L5 is a novel member of the mammalian CRB-MPP5 polarity complex

Ilse Gosens, Alessandro Sessa, Anneke I. den Hollander, Stef J F Letteboer, Valentina Belloni, Maarten L. Arends, André Le Bivic, Frans P M Cremers, Vania Broccoli, Ronald Roepman

Research output: Contribution to journalArticlepeer-review

Abstract

Cell polarity is induced and maintained by separation of the apical and basolateral domains through specialized cell-cell junctions. The Crumbs protein and its binding partners are involved in formation and stabilization of adherens junctions. In this study, we describe a novel component of the mammalian Crumbs complex, the FERM domain protein EPB41L5, which associates with the intracellular domains of all three Crumbs homologs through its FERM domain. Surprisingly, the same FERM domain is involved in binding to the HOOK domain of MPP5/PALS1, a previously identified interactor of Crumbs. Co-expression and co-localization studies suggested that in several epithelial derived tissues Epb4.1l5 interacts with at least one Crumbs homolog, and with Mpp5. Although at early embryonic stages Epb4.1l5 is found at the basolateral membrane compartment, in adult tissues it co-localizes at the apical domain with Crumbs proteins and Mpp5. Overexpression of Epb4.1l5 in polarized MDCK cells affects tightness of cell junctions and results in disorganization of the tight junction markers ZO-1 and PATJ. Our results emphasize the importance of a conserved Crumbs-MPP5-EPB41L5 polarity complex in mammals.

Original languageEnglish
Pages (from-to)3959-3970
Number of pages12
JournalExperimental Cell Research
Volume313
Issue number19
DOIs
Publication statusPublished - Nov 15 2007

Keywords

  • Cell polarity
  • Crumbs
  • EPB41L5/mosaic eyes/YMO1
  • FERM domain
  • MAGUK
  • MPP5/Pals1

ASJC Scopus subject areas

  • Cell Biology

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