TY - JOUR
T1 - Fetal Cardiac Impairment in Nitrofen-Induced Congenital Diaphragmatic Hernia
T2 - Postmortem Microcomputed Tomography Imaging Study
AU - Pelizzo, Gloria
AU - Calcaterra, Valeria
AU - Lombardi, Claudio
AU - Bussani, Rossana
AU - Zambelli, Vanessa
AU - De Silvestri, Annalisa
AU - Custrin, Ana
AU - Belgrano, Manuel
AU - Zennaro, Floriana
PY - 2017/7/4
Y1 - 2017/7/4
N2 - Introduction: We assessed the post-mortem micro-CT utility to evaluate fetal cardiac impairment in nitrofen-induced congenital diaphragmatic hernia (CDH). Methods: At 9.5d postconception (dpc), pregnant rats were exposed to nitrofen. At +18 and +21dpc, fetuses were harvested by cesarean section. Postmortem micro-CT and autopsy were performed. Fetuses were assigned to three experimental groups: Control group (C), Nitrofen group (N, exposition to nitrofen without CDH), CDH group. Cardio-pulmonary indices were evaluated. Results: An accurate morphological evaluation of the lung and heart was obtained. Early cardiac impairment was present in the N and CDH groups. At term pregnancy, an increased maximum diameter and decreased minimum diameter of the ventricles and increased interventricular septal thickness were noted in CDH. Histology showed a myocardial “disarray” and an high density of mitotic myocytes in CDH at midgestation. Conclusions: The potential utility of post-mortem fetal micro-CT examination in CDH was introduced. The results highlighted the presence of cardiac adaptation in affected fetuses.
AB - Introduction: We assessed the post-mortem micro-CT utility to evaluate fetal cardiac impairment in nitrofen-induced congenital diaphragmatic hernia (CDH). Methods: At 9.5d postconception (dpc), pregnant rats were exposed to nitrofen. At +18 and +21dpc, fetuses were harvested by cesarean section. Postmortem micro-CT and autopsy were performed. Fetuses were assigned to three experimental groups: Control group (C), Nitrofen group (N, exposition to nitrofen without CDH), CDH group. Cardio-pulmonary indices were evaluated. Results: An accurate morphological evaluation of the lung and heart was obtained. Early cardiac impairment was present in the N and CDH groups. At term pregnancy, an increased maximum diameter and decreased minimum diameter of the ventricles and increased interventricular septal thickness were noted in CDH. Histology showed a myocardial “disarray” and an high density of mitotic myocytes in CDH at midgestation. Conclusions: The potential utility of post-mortem fetal micro-CT examination in CDH was introduced. The results highlighted the presence of cardiac adaptation in affected fetuses.
KW - Congenital diaphragmatic hernia
KW - fetal cardiac impairment
KW - microcomputed tomography
KW - nitrofen
KW - virtual autopsy
UR - http://www.scopus.com/inward/record.url?scp=85019046154&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019046154&partnerID=8YFLogxK
U2 - 10.1080/15513815.2017.1315198
DO - 10.1080/15513815.2017.1315198
M3 - Article
AN - SCOPUS:85019046154
VL - 36
SP - 282
EP - 293
JO - Fetal and Pediatric Pathology
JF - Fetal and Pediatric Pathology
SN - 1551-3815
IS - 4
ER -