Fetuin-A gene expression, synthesis and release in primary human hepatocytes cultured in a galactosylated membrane bioreactor

Bruno Memoli, Loredana De Bartolo, Pietro Favia, Sabrina Morelli, Linda C. Lopez, Alfredo Procino, Giuseppe Barbieri, Efrem Curcio, Lidietta Giorno, Pasquale Esposito, Mario Cozzolino, Diego Brancaccio, Vittorio E. Andreucci, Riccardo d'Agostino, Enrico Drioli

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

This paper reports on human hepatocytes cultured in a galactosylated membrane bioreactor in order to explore the modulation of the effects of a pro-inflammatory cytokine, Interleukin-6 (IL-6) on the liver cells at molecular level. In particular the role of IL-6 on gene expression and production of a glycoprotein, fetuin-A produced by hepatocytes, was investigated by culturing hepatocytes in the membrane bioreactor, both in the absence and presence of IL-6 (300 pg/ml). IL-6 modulated the fetuin-A gene expression, synthesis and release by primary human hepatocytes cultured in the bioreactor. A 75% IL-6-induced reduction of fetuin-A concentration in the medium was associated with a 60% increase of C-reactive protein in the same samples. Real-time-PCR demonstrated an 8-fold IL-6-induced reduction of fetuin-A gene expression. These results demonstrate that the hepatocyte galactosylated membrane bioreactor is a valuable tool to study IL-6 effects and gave evidence, for the first time, that IL-6 down-regulates the gene expression and synthesis of fetuin-A by primary human hepatocytes. The human hepatocyte bioreactor behaves like the in vivo liver, reproducing the same hepatic acute-phase response that occurs during the inflammatory process.

Original languageEnglish
Pages (from-to)4836-4844
Number of pages9
JournalBiomaterials
Volume28
Issue number32
DOIs
Publication statusPublished - Nov 2007

Fingerprint

alpha-2-HS-Glycoprotein
Bioreactors
Gene expression
Hepatocytes
Interleukin-6
Membranes
Gene Expression
Liver
Glycoproteins
Acute-Phase Reaction
C-Reactive Protein
Real-Time Polymerase Chain Reaction
Modulation
Down-Regulation
Proteins
Cytokines

Keywords

  • Bioreactor
  • Fetuin-A
  • Galactosylated membrane
  • Hepatocytes
  • IL-6
  • Inflammation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Memoli, B., De Bartolo, L., Favia, P., Morelli, S., Lopez, L. C., Procino, A., ... Drioli, E. (2007). Fetuin-A gene expression, synthesis and release in primary human hepatocytes cultured in a galactosylated membrane bioreactor. Biomaterials, 28(32), 4836-4844. https://doi.org/10.1016/j.biomaterials.2007.05.043

Fetuin-A gene expression, synthesis and release in primary human hepatocytes cultured in a galactosylated membrane bioreactor. / Memoli, Bruno; De Bartolo, Loredana; Favia, Pietro; Morelli, Sabrina; Lopez, Linda C.; Procino, Alfredo; Barbieri, Giuseppe; Curcio, Efrem; Giorno, Lidietta; Esposito, Pasquale; Cozzolino, Mario; Brancaccio, Diego; Andreucci, Vittorio E.; d'Agostino, Riccardo; Drioli, Enrico.

In: Biomaterials, Vol. 28, No. 32, 11.2007, p. 4836-4844.

Research output: Contribution to journalArticle

Memoli, B, De Bartolo, L, Favia, P, Morelli, S, Lopez, LC, Procino, A, Barbieri, G, Curcio, E, Giorno, L, Esposito, P, Cozzolino, M, Brancaccio, D, Andreucci, VE, d'Agostino, R & Drioli, E 2007, 'Fetuin-A gene expression, synthesis and release in primary human hepatocytes cultured in a galactosylated membrane bioreactor', Biomaterials, vol. 28, no. 32, pp. 4836-4844. https://doi.org/10.1016/j.biomaterials.2007.05.043
Memoli, Bruno ; De Bartolo, Loredana ; Favia, Pietro ; Morelli, Sabrina ; Lopez, Linda C. ; Procino, Alfredo ; Barbieri, Giuseppe ; Curcio, Efrem ; Giorno, Lidietta ; Esposito, Pasquale ; Cozzolino, Mario ; Brancaccio, Diego ; Andreucci, Vittorio E. ; d'Agostino, Riccardo ; Drioli, Enrico. / Fetuin-A gene expression, synthesis and release in primary human hepatocytes cultured in a galactosylated membrane bioreactor. In: Biomaterials. 2007 ; Vol. 28, No. 32. pp. 4836-4844.
@article{5bd82dfff1f7449cbd75160b2dfea59e,
title = "Fetuin-A gene expression, synthesis and release in primary human hepatocytes cultured in a galactosylated membrane bioreactor",
abstract = "This paper reports on human hepatocytes cultured in a galactosylated membrane bioreactor in order to explore the modulation of the effects of a pro-inflammatory cytokine, Interleukin-6 (IL-6) on the liver cells at molecular level. In particular the role of IL-6 on gene expression and production of a glycoprotein, fetuin-A produced by hepatocytes, was investigated by culturing hepatocytes in the membrane bioreactor, both in the absence and presence of IL-6 (300 pg/ml). IL-6 modulated the fetuin-A gene expression, synthesis and release by primary human hepatocytes cultured in the bioreactor. A 75{\%} IL-6-induced reduction of fetuin-A concentration in the medium was associated with a 60{\%} increase of C-reactive protein in the same samples. Real-time-PCR demonstrated an 8-fold IL-6-induced reduction of fetuin-A gene expression. These results demonstrate that the hepatocyte galactosylated membrane bioreactor is a valuable tool to study IL-6 effects and gave evidence, for the first time, that IL-6 down-regulates the gene expression and synthesis of fetuin-A by primary human hepatocytes. The human hepatocyte bioreactor behaves like the in vivo liver, reproducing the same hepatic acute-phase response that occurs during the inflammatory process.",
keywords = "Bioreactor, Fetuin-A, Galactosylated membrane, Hepatocytes, IL-6, Inflammation",
author = "Bruno Memoli and {De Bartolo}, Loredana and Pietro Favia and Sabrina Morelli and Lopez, {Linda C.} and Alfredo Procino and Giuseppe Barbieri and Efrem Curcio and Lidietta Giorno and Pasquale Esposito and Mario Cozzolino and Diego Brancaccio and Andreucci, {Vittorio E.} and Riccardo d'Agostino and Enrico Drioli",
year = "2007",
month = "11",
doi = "10.1016/j.biomaterials.2007.05.043",
language = "English",
volume = "28",
pages = "4836--4844",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "32",

}

TY - JOUR

T1 - Fetuin-A gene expression, synthesis and release in primary human hepatocytes cultured in a galactosylated membrane bioreactor

AU - Memoli, Bruno

AU - De Bartolo, Loredana

AU - Favia, Pietro

AU - Morelli, Sabrina

AU - Lopez, Linda C.

AU - Procino, Alfredo

AU - Barbieri, Giuseppe

AU - Curcio, Efrem

AU - Giorno, Lidietta

AU - Esposito, Pasquale

AU - Cozzolino, Mario

AU - Brancaccio, Diego

AU - Andreucci, Vittorio E.

AU - d'Agostino, Riccardo

AU - Drioli, Enrico

PY - 2007/11

Y1 - 2007/11

N2 - This paper reports on human hepatocytes cultured in a galactosylated membrane bioreactor in order to explore the modulation of the effects of a pro-inflammatory cytokine, Interleukin-6 (IL-6) on the liver cells at molecular level. In particular the role of IL-6 on gene expression and production of a glycoprotein, fetuin-A produced by hepatocytes, was investigated by culturing hepatocytes in the membrane bioreactor, both in the absence and presence of IL-6 (300 pg/ml). IL-6 modulated the fetuin-A gene expression, synthesis and release by primary human hepatocytes cultured in the bioreactor. A 75% IL-6-induced reduction of fetuin-A concentration in the medium was associated with a 60% increase of C-reactive protein in the same samples. Real-time-PCR demonstrated an 8-fold IL-6-induced reduction of fetuin-A gene expression. These results demonstrate that the hepatocyte galactosylated membrane bioreactor is a valuable tool to study IL-6 effects and gave evidence, for the first time, that IL-6 down-regulates the gene expression and synthesis of fetuin-A by primary human hepatocytes. The human hepatocyte bioreactor behaves like the in vivo liver, reproducing the same hepatic acute-phase response that occurs during the inflammatory process.

AB - This paper reports on human hepatocytes cultured in a galactosylated membrane bioreactor in order to explore the modulation of the effects of a pro-inflammatory cytokine, Interleukin-6 (IL-6) on the liver cells at molecular level. In particular the role of IL-6 on gene expression and production of a glycoprotein, fetuin-A produced by hepatocytes, was investigated by culturing hepatocytes in the membrane bioreactor, both in the absence and presence of IL-6 (300 pg/ml). IL-6 modulated the fetuin-A gene expression, synthesis and release by primary human hepatocytes cultured in the bioreactor. A 75% IL-6-induced reduction of fetuin-A concentration in the medium was associated with a 60% increase of C-reactive protein in the same samples. Real-time-PCR demonstrated an 8-fold IL-6-induced reduction of fetuin-A gene expression. These results demonstrate that the hepatocyte galactosylated membrane bioreactor is a valuable tool to study IL-6 effects and gave evidence, for the first time, that IL-6 down-regulates the gene expression and synthesis of fetuin-A by primary human hepatocytes. The human hepatocyte bioreactor behaves like the in vivo liver, reproducing the same hepatic acute-phase response that occurs during the inflammatory process.

KW - Bioreactor

KW - Fetuin-A

KW - Galactosylated membrane

KW - Hepatocytes

KW - IL-6

KW - Inflammation

UR - http://www.scopus.com/inward/record.url?scp=34548022299&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548022299&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2007.05.043

DO - 10.1016/j.biomaterials.2007.05.043

M3 - Article

C2 - 17706279

AN - SCOPUS:34548022299

VL - 28

SP - 4836

EP - 4844

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 32

ER -