FGF-2 stimulates migration of Kaposi's sarcoma-like vascular cells by HGF-dependent relocalization of the urokinase receptor

Ugo Cavallaro, Zhihao Wu, Andrea Di Palo, Roberto Montesano, Michael S. Pepper, Jeanette A M Maier, Marco R. Soria

Research output: Contribution to journalArticle

Abstract

The spindle-shaped cell line TTB was recently isolated from highly vascularized skin lesions of BKV/HIV-1 tat transgenic mice and shown to possess an autocrine loop for hepatocyte growth factor (HGF). We show that fibroblast growth factor-2 (FGF-2) stimulates TTB cell migration and promotes polarization of uPAR at the leading edge of migrating cells. FGF-stimulated TTB cells presented the typical migratory phenotype, with a triangular cell shape and concomitant breakdown of actin stress fibers and smooth muscle- specific actin isoform. FGF-2-stimulated migration was blocked by antibodies against urokinase-type plasminogen activator (uPA) or uLPA receptor (uPAR) and by neutralizing anti-HGF antibodies. The latter also inhibited uPAR relocalization at the cell surface of FGF-2-treated TTB cells. This points to a crosstalk between FGF-2 and HGF that might mediate TTB cell migration by modulating the localization of cell surface uPAR.

Original languageEnglish
Pages (from-to)1027-1034
Number of pages8
JournalFASEB Journal
Volume12
Issue number11
Publication statusPublished - 1998

Fingerprint

hepatocyte growth factor
u-plasminogen activator
fibroblast growth factor 2
Hepatocyte Growth Factor
Kaposi's Sarcoma
Urokinase-Type Plasminogen Activator
sarcoma
Fibroblast Growth Factor 2
blood vessels
Blood Vessels
Cells
receptors
Cell Movement
Actins
cells
cell movement
Stress Fibers
actin
Cell Shape
Antibodies

Keywords

  • FGF
  • Hepatocyte growth factor
  • TTB cell migration
  • UPAR relocalization

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Cavallaro, U., Wu, Z., Di Palo, A., Montesano, R., Pepper, M. S., Maier, J. A. M., & Soria, M. R. (1998). FGF-2 stimulates migration of Kaposi's sarcoma-like vascular cells by HGF-dependent relocalization of the urokinase receptor. FASEB Journal, 12(11), 1027-1034.

FGF-2 stimulates migration of Kaposi's sarcoma-like vascular cells by HGF-dependent relocalization of the urokinase receptor. / Cavallaro, Ugo; Wu, Zhihao; Di Palo, Andrea; Montesano, Roberto; Pepper, Michael S.; Maier, Jeanette A M; Soria, Marco R.

In: FASEB Journal, Vol. 12, No. 11, 1998, p. 1027-1034.

Research output: Contribution to journalArticle

Cavallaro, U, Wu, Z, Di Palo, A, Montesano, R, Pepper, MS, Maier, JAM & Soria, MR 1998, 'FGF-2 stimulates migration of Kaposi's sarcoma-like vascular cells by HGF-dependent relocalization of the urokinase receptor', FASEB Journal, vol. 12, no. 11, pp. 1027-1034.
Cavallaro, Ugo ; Wu, Zhihao ; Di Palo, Andrea ; Montesano, Roberto ; Pepper, Michael S. ; Maier, Jeanette A M ; Soria, Marco R. / FGF-2 stimulates migration of Kaposi's sarcoma-like vascular cells by HGF-dependent relocalization of the urokinase receptor. In: FASEB Journal. 1998 ; Vol. 12, No. 11. pp. 1027-1034.
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