Abstract
The spindle-shaped cell line TTB was recently isolated from highly vascularized skin lesions of BKV/HIV-1 tat transgenic mice and shown to possess an autocrine loop for hepatocyte growth factor (HGF). We show that fibroblast growth factor-2 (FGF-2) stimulates TTB cell migration and promotes polarization of uPAR at the leading edge of migrating cells. FGF-stimulated TTB cells presented the typical migratory phenotype, with a triangular cell shape and concomitant breakdown of actin stress fibers and smooth muscle- specific actin isoform. FGF-2-stimulated migration was blocked by antibodies against urokinase-type plasminogen activator (uPA) or uLPA receptor (uPAR) and by neutralizing anti-HGF antibodies. The latter also inhibited uPAR relocalization at the cell surface of FGF-2-treated TTB cells. This points to a crosstalk between FGF-2 and HGF that might mediate TTB cell migration by modulating the localization of cell surface uPAR.
| Original language | English |
|---|---|
| Pages (from-to) | 1027-1034 |
| Number of pages | 8 |
| Journal | FASEB Journal |
| Volume | 12 |
| Issue number | 11 |
| Publication status | Published - 1998 |
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Keywords
- FGF
- Hepatocyte growth factor
- TTB cell migration
- UPAR relocalization
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
Cite this
FGF-2 stimulates migration of Kaposi's sarcoma-like vascular cells by HGF-dependent relocalization of the urokinase receptor. / Cavallaro, Ugo; Wu, Zhihao; Di Palo, Andrea; Montesano, Roberto; Pepper, Michael S.; Maier, Jeanette A M; Soria, Marco R.
In: FASEB Journal, Vol. 12, No. 11, 1998, p. 1027-1034.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - FGF-2 stimulates migration of Kaposi's sarcoma-like vascular cells by HGF-dependent relocalization of the urokinase receptor
AU - Cavallaro, Ugo
AU - Wu, Zhihao
AU - Di Palo, Andrea
AU - Montesano, Roberto
AU - Pepper, Michael S.
AU - Maier, Jeanette A M
AU - Soria, Marco R.
PY - 1998
Y1 - 1998
N2 - The spindle-shaped cell line TTB was recently isolated from highly vascularized skin lesions of BKV/HIV-1 tat transgenic mice and shown to possess an autocrine loop for hepatocyte growth factor (HGF). We show that fibroblast growth factor-2 (FGF-2) stimulates TTB cell migration and promotes polarization of uPAR at the leading edge of migrating cells. FGF-stimulated TTB cells presented the typical migratory phenotype, with a triangular cell shape and concomitant breakdown of actin stress fibers and smooth muscle- specific actin isoform. FGF-2-stimulated migration was blocked by antibodies against urokinase-type plasminogen activator (uPA) or uLPA receptor (uPAR) and by neutralizing anti-HGF antibodies. The latter also inhibited uPAR relocalization at the cell surface of FGF-2-treated TTB cells. This points to a crosstalk between FGF-2 and HGF that might mediate TTB cell migration by modulating the localization of cell surface uPAR.
AB - The spindle-shaped cell line TTB was recently isolated from highly vascularized skin lesions of BKV/HIV-1 tat transgenic mice and shown to possess an autocrine loop for hepatocyte growth factor (HGF). We show that fibroblast growth factor-2 (FGF-2) stimulates TTB cell migration and promotes polarization of uPAR at the leading edge of migrating cells. FGF-stimulated TTB cells presented the typical migratory phenotype, with a triangular cell shape and concomitant breakdown of actin stress fibers and smooth muscle- specific actin isoform. FGF-2-stimulated migration was blocked by antibodies against urokinase-type plasminogen activator (uPA) or uLPA receptor (uPAR) and by neutralizing anti-HGF antibodies. The latter also inhibited uPAR relocalization at the cell surface of FGF-2-treated TTB cells. This points to a crosstalk between FGF-2 and HGF that might mediate TTB cell migration by modulating the localization of cell surface uPAR.
KW - FGF
KW - Hepatocyte growth factor
KW - TTB cell migration
KW - UPAR relocalization
UR - http://www.scopus.com/inward/record.url?scp=0031854841&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031854841&partnerID=8YFLogxK
M3 - Article
C2 - 9707175
AN - SCOPUS:0031854841
VL - 12
SP - 1027
EP - 1034
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 11
ER -