Fgf8 dosage determines midfacial integration and polarity within the nasal and optic capsules

John N. Griffin, Claudia Compagnucci, Diane Hu, Jennifer Fish, Ophir Klein, Ralph Marcucio, Michael J. Depew

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Craniofacial development requires an exquisitely timed and positioned cross-talk between the embryonic cephalic epithelia and mesenchyme. This cross-talk underlies the precise translation of patterning processes and information into distinct, appropriate skeletal morphologies. The molecular and cellular dialogue includes communication via secreted signaling molecules, including Fgf8, and effectors of their interpretation. Herein, we use genetic attenuation of Fgf8 in mice and perform gain-of-function mouse-chick chimeric experiments to demonstrate that significant character states of the frontonasal and optic skeletons are dependent on Fgf8. Notably, we show that the normal orientation and polarity of the nasal capsules and their developing primordia are dependent on Fgf8. We further demonstrate that Fgf8 is required for midfacial integration, and provide evidence for a role for Fgf8 in optic capsular development. Taken together, our data highlight Fgf8 signaling in craniofacial development as a plausible target for evolutionary selective pressures.

Original languageEnglish
Pages (from-to)185-197
Number of pages13
JournalDevelopmental Biology
Volume374
Issue number1
DOIs
Publication statusPublished - Feb 1 2013

Fingerprint

Nose
Capsules
Mesoderm
Skeleton
Epithelium
Communication
Head

Keywords

  • Craniofacial
  • Ectoderm
  • FEZ
  • Fgf8
  • Midface
  • Mouse
  • Nasal capsule
  • Optic capsule
  • Trabecular basal plate

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Fgf8 dosage determines midfacial integration and polarity within the nasal and optic capsules. / Griffin, John N.; Compagnucci, Claudia; Hu, Diane; Fish, Jennifer; Klein, Ophir; Marcucio, Ralph; Depew, Michael J.

In: Developmental Biology, Vol. 374, No. 1, 01.02.2013, p. 185-197.

Research output: Contribution to journalArticle

Griffin, John N. ; Compagnucci, Claudia ; Hu, Diane ; Fish, Jennifer ; Klein, Ophir ; Marcucio, Ralph ; Depew, Michael J. / Fgf8 dosage determines midfacial integration and polarity within the nasal and optic capsules. In: Developmental Biology. 2013 ; Vol. 374, No. 1. pp. 185-197.
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