Abstract
This study aimed at evaluating by a quantitative assay the fibrin clot retractile activity (FCR) of C3H embryo fibroblasts during their growth and aging in culture. Cell from primary and subsequent subcultures were tested at defined times from seeding, in a specially devised micromethod. Results indicate that cell-induced FCR has a kinetic similar to platelet-induced FCR; it depends on the number of cells and time of incubation in the system. It is absent or low in cells harvested from primary culture, then increases and remains high in the following doublings decreasing sharply at the end of the replicative life span in culture.
| Original language | English |
|---|---|
| Pages (from-to) | 731-737 |
| Number of pages | 7 |
| Journal | In Vitro |
| Volume | 16 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 1980 |
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Keywords
- aging
- cell growth
- fibrin clot retraction
- fibroblasts
ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry
- Developmental Biology
Cite this
Fibrin clot retractile activity of mouse fibroblasts during growth and aging. / Curatolo, L.; Balconi, G.; Borgia, R.; Morasca, L.; Donati, M. B.
In: In Vitro, Vol. 16, No. 9, 1980, p. 731-737.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fibrin clot retractile activity of mouse fibroblasts during growth and aging
AU - Curatolo, L.
AU - Balconi, G.
AU - Borgia, R.
AU - Morasca, L.
AU - Donati, M. B.
PY - 1980
Y1 - 1980
N2 - This study aimed at evaluating by a quantitative assay the fibrin clot retractile activity (FCR) of C3H embryo fibroblasts during their growth and aging in culture. Cell from primary and subsequent subcultures were tested at defined times from seeding, in a specially devised micromethod. Results indicate that cell-induced FCR has a kinetic similar to platelet-induced FCR; it depends on the number of cells and time of incubation in the system. It is absent or low in cells harvested from primary culture, then increases and remains high in the following doublings decreasing sharply at the end of the replicative life span in culture.
AB - This study aimed at evaluating by a quantitative assay the fibrin clot retractile activity (FCR) of C3H embryo fibroblasts during their growth and aging in culture. Cell from primary and subsequent subcultures were tested at defined times from seeding, in a specially devised micromethod. Results indicate that cell-induced FCR has a kinetic similar to platelet-induced FCR; it depends on the number of cells and time of incubation in the system. It is absent or low in cells harvested from primary culture, then increases and remains high in the following doublings decreasing sharply at the end of the replicative life span in culture.
KW - aging
KW - cell growth
KW - fibrin clot retraction
KW - fibroblasts
UR - http://www.scopus.com/inward/record.url?scp=0019164154&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019164154&partnerID=8YFLogxK
U2 - 10.1007/BF02619306
DO - 10.1007/BF02619306
M3 - Article
C2 - 7409833
AN - SCOPUS:0019164154
VL - 16
SP - 731
EP - 737
JO - In Vitro Cellular and Developmental Biology - Plant
JF - In Vitro Cellular and Developmental Biology - Plant
SN - 1054-5476
IS - 9
ER -