Fibrinogen assays

A collaborative study of six different methods

G. Palareti, M. Maccaferri, C. Manotti, A. Tripodi, V. Chantarangkul, F. Rodeghiero, M. Ruggeri, P. M. Mannucci

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The same frozen plasma samples, with normal and high contents of fibrinogen, were examined in four laboratories. The methods were calibrated with an internal standard whose fibrinogen content was determined gravimetrically. Both the Von Clauss and the RID methods were reliable, accurate, and precise, if adequate calibration was used. The PT-derived method was highly reproducible, but had some problems with accuracy. We demonstrate that an adequate calibration procedure is indispensable for reliable fibrinogen measurements whatever method is used. Because neither the calibration procedures proposed by the manufacturers nor the use of lyophilized commercial plasmas is adequate for this purpose, we urge that an international standard for fibrinogen measurement be promptly established.

Original languageEnglish
Pages (from-to)714-719
Number of pages6
JournalClinical Chemistry
Volume37
Issue number5
Publication statusPublished - 1991

Fingerprint

Fibrinogen
Assays
Calibration
Plasmas

Keywords

  • Calibration
  • Coagulation
  • Intermethod comparison

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Palareti, G., Maccaferri, M., Manotti, C., Tripodi, A., Chantarangkul, V., Rodeghiero, F., ... Mannucci, P. M. (1991). Fibrinogen assays: A collaborative study of six different methods. Clinical Chemistry, 37(5), 714-719.

Fibrinogen assays : A collaborative study of six different methods. / Palareti, G.; Maccaferri, M.; Manotti, C.; Tripodi, A.; Chantarangkul, V.; Rodeghiero, F.; Ruggeri, M.; Mannucci, P. M.

In: Clinical Chemistry, Vol. 37, No. 5, 1991, p. 714-719.

Research output: Contribution to journalArticle

Palareti, G, Maccaferri, M, Manotti, C, Tripodi, A, Chantarangkul, V, Rodeghiero, F, Ruggeri, M & Mannucci, PM 1991, 'Fibrinogen assays: A collaborative study of six different methods', Clinical Chemistry, vol. 37, no. 5, pp. 714-719.
Palareti G, Maccaferri M, Manotti C, Tripodi A, Chantarangkul V, Rodeghiero F et al. Fibrinogen assays: A collaborative study of six different methods. Clinical Chemistry. 1991;37(5):714-719.
Palareti, G. ; Maccaferri, M. ; Manotti, C. ; Tripodi, A. ; Chantarangkul, V. ; Rodeghiero, F. ; Ruggeri, M. ; Mannucci, P. M. / Fibrinogen assays : A collaborative study of six different methods. In: Clinical Chemistry. 1991 ; Vol. 37, No. 5. pp. 714-719.
@article{dbcfdc07c4bd4fcba0a1f958e122b7b4,
title = "Fibrinogen assays: A collaborative study of six different methods",
abstract = "The same frozen plasma samples, with normal and high contents of fibrinogen, were examined in four laboratories. The methods were calibrated with an internal standard whose fibrinogen content was determined gravimetrically. Both the Von Clauss and the RID methods were reliable, accurate, and precise, if adequate calibration was used. The PT-derived method was highly reproducible, but had some problems with accuracy. We demonstrate that an adequate calibration procedure is indispensable for reliable fibrinogen measurements whatever method is used. Because neither the calibration procedures proposed by the manufacturers nor the use of lyophilized commercial plasmas is adequate for this purpose, we urge that an international standard for fibrinogen measurement be promptly established.",
keywords = "Calibration, Coagulation, Intermethod comparison",
author = "G. Palareti and M. Maccaferri and C. Manotti and A. Tripodi and V. Chantarangkul and F. Rodeghiero and M. Ruggeri and Mannucci, {P. M.}",
year = "1991",
language = "English",
volume = "37",
pages = "714--719",
journal = "Clinical Chemistry",
issn = "0009-9147",
publisher = "American Association for Clinical Chemistry Inc.",
number = "5",

}

TY - JOUR

T1 - Fibrinogen assays

T2 - A collaborative study of six different methods

AU - Palareti, G.

AU - Maccaferri, M.

AU - Manotti, C.

AU - Tripodi, A.

AU - Chantarangkul, V.

AU - Rodeghiero, F.

AU - Ruggeri, M.

AU - Mannucci, P. M.

PY - 1991

Y1 - 1991

N2 - The same frozen plasma samples, with normal and high contents of fibrinogen, were examined in four laboratories. The methods were calibrated with an internal standard whose fibrinogen content was determined gravimetrically. Both the Von Clauss and the RID methods were reliable, accurate, and precise, if adequate calibration was used. The PT-derived method was highly reproducible, but had some problems with accuracy. We demonstrate that an adequate calibration procedure is indispensable for reliable fibrinogen measurements whatever method is used. Because neither the calibration procedures proposed by the manufacturers nor the use of lyophilized commercial plasmas is adequate for this purpose, we urge that an international standard for fibrinogen measurement be promptly established.

AB - The same frozen plasma samples, with normal and high contents of fibrinogen, were examined in four laboratories. The methods were calibrated with an internal standard whose fibrinogen content was determined gravimetrically. Both the Von Clauss and the RID methods were reliable, accurate, and precise, if adequate calibration was used. The PT-derived method was highly reproducible, but had some problems with accuracy. We demonstrate that an adequate calibration procedure is indispensable for reliable fibrinogen measurements whatever method is used. Because neither the calibration procedures proposed by the manufacturers nor the use of lyophilized commercial plasmas is adequate for this purpose, we urge that an international standard for fibrinogen measurement be promptly established.

KW - Calibration

KW - Coagulation

KW - Intermethod comparison

UR - http://www.scopus.com/inward/record.url?scp=0025768022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025768022&partnerID=8YFLogxK

M3 - Article

VL - 37

SP - 714

EP - 719

JO - Clinical Chemistry

JF - Clinical Chemistry

SN - 0009-9147

IS - 5

ER -