Abstract
The same frozen plasma samples, with normal and high contents of fibrinogen, were examined in four laboratories. The methods were calibrated with an internal standard whose fibrinogen content was determined gravimetrically. Both the Von Clauss and the RID methods were reliable, accurate, and precise, if adequate calibration was used. The PT-derived method was highly reproducible, but had some problems with accuracy. We demonstrate that an adequate calibration procedure is indispensable for reliable fibrinogen measurements whatever method is used. Because neither the calibration procedures proposed by the manufacturers nor the use of lyophilized commercial plasmas is adequate for this purpose, we urge that an international standard for fibrinogen measurement be promptly established.
| Original language | English |
|---|---|
| Pages (from-to) | 714-719 |
| Number of pages | 6 |
| Journal | Clinical Chemistry |
| Volume | 37 |
| Issue number | 5 |
| Publication status | Published - 1991 |
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Keywords
- Calibration
- Coagulation
- Intermethod comparison
ASJC Scopus subject areas
- Clinical Biochemistry
Cite this
Fibrinogen assays : A collaborative study of six different methods. / Palareti, G.; Maccaferri, M.; Manotti, C.; Tripodi, A.; Chantarangkul, V.; Rodeghiero, F.; Ruggeri, M.; Mannucci, P. M.
In: Clinical Chemistry, Vol. 37, No. 5, 1991, p. 714-719.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Fibrinogen assays
T2 - A collaborative study of six different methods
AU - Palareti, G.
AU - Maccaferri, M.
AU - Manotti, C.
AU - Tripodi, A.
AU - Chantarangkul, V.
AU - Rodeghiero, F.
AU - Ruggeri, M.
AU - Mannucci, P. M.
PY - 1991
Y1 - 1991
N2 - The same frozen plasma samples, with normal and high contents of fibrinogen, were examined in four laboratories. The methods were calibrated with an internal standard whose fibrinogen content was determined gravimetrically. Both the Von Clauss and the RID methods were reliable, accurate, and precise, if adequate calibration was used. The PT-derived method was highly reproducible, but had some problems with accuracy. We demonstrate that an adequate calibration procedure is indispensable for reliable fibrinogen measurements whatever method is used. Because neither the calibration procedures proposed by the manufacturers nor the use of lyophilized commercial plasmas is adequate for this purpose, we urge that an international standard for fibrinogen measurement be promptly established.
AB - The same frozen plasma samples, with normal and high contents of fibrinogen, were examined in four laboratories. The methods were calibrated with an internal standard whose fibrinogen content was determined gravimetrically. Both the Von Clauss and the RID methods were reliable, accurate, and precise, if adequate calibration was used. The PT-derived method was highly reproducible, but had some problems with accuracy. We demonstrate that an adequate calibration procedure is indispensable for reliable fibrinogen measurements whatever method is used. Because neither the calibration procedures proposed by the manufacturers nor the use of lyophilized commercial plasmas is adequate for this purpose, we urge that an international standard for fibrinogen measurement be promptly established.
KW - Calibration
KW - Coagulation
KW - Intermethod comparison
UR - http://www.scopus.com/inward/record.url?scp=0025768022&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025768022&partnerID=8YFLogxK
M3 - Article
VL - 37
SP - 714
EP - 719
JO - Clinical Chemistry
JF - Clinical Chemistry
SN - 0009-9147
IS - 5
ER -