Fibrinogen assays: A collaborative study of six different methods

G. Palareti, M. Maccaferri, C. Manotti, A. Tripodi, V. Chantarangkul, F. Rodeghiero, M. Ruggeri, P. M. Mannucci

Research output: Contribution to journalArticlepeer-review


The same frozen plasma samples, with normal and high contents of fibrinogen, were examined in four laboratories. The methods were calibrated with an internal standard whose fibrinogen content was determined gravimetrically. Both the Von Clauss and the RID methods were reliable, accurate, and precise, if adequate calibration was used. The PT-derived method was highly reproducible, but had some problems with accuracy. We demonstrate that an adequate calibration procedure is indispensable for reliable fibrinogen measurements whatever method is used. Because neither the calibration procedures proposed by the manufacturers nor the use of lyophilized commercial plasmas is adequate for this purpose, we urge that an international standard for fibrinogen measurement be promptly established.

Original languageEnglish
Pages (from-to)714-719
Number of pages6
JournalClinical Chemistry
Issue number5
Publication statusPublished - 1991


  • Calibration
  • Coagulation
  • Intermethod comparison

ASJC Scopus subject areas

  • Clinical Biochemistry


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