Fibroblasts increase adhesion to neutrophils after stimulation with phorbol ester and cytokines

A. L. Giuliani, S. Spisani, T. Cavalletti, E. Reali, L. Melchiorri, L. Ferrari, F. Lanza, S. Traniello

Research output: Contribution to journalArticle

Abstract

In our research there was spontaneous adhesion between resting fibroblasts and neutrophils in vitro which could be increased by stimulating either the coculture of cells or each cell type separately with various stimulants. Interferon-γ, interleukin-1, and interleukin-6 significantly increased adhesion; however, the highest adhesive response was obtained when cocultures were treated with phorbol myristate acetate (PMA). As PMA-stimulated fibroblasts show the highest adhesion to resting neutrophils, it was suggested that adhesion was primarily due to an effect on fibroblasts. Without Mg2+ PMA did not stimulate fibroblast adhesion, whereas in the absence of Ca2+ the response was only partially reduced. adhesion was independent of both neutrophil integrins and fibroblast ICAM-1, whereas cytokine-stimulated adhesion was blocked by mAbs against ICAM-1; PMA-stimulated adhesion was not affected by mAbs anti-ICAM-1, but was partially inhibited by mAbs anti-β2 integrins. These results suggested the presence of mechanisms able to modulate the adhesive fibroblast-neutrophil interaction in inflammatory and wound healing processes.

Original languageEnglish
Pages (from-to)208-222
Number of pages15
JournalCellular Immunology
Volume149
Issue number1
DOIs
Publication statusPublished - 1993

Fingerprint

Phorbol Esters
Neutrophils
Fibroblasts
Tetradecanoylphorbol Acetate
Cytokines
Intercellular Adhesion Molecule-1
Coculture Techniques
Integrins
Adhesives
Interleukin-1
Wound Healing
Interferons
Interleukin-6
Research

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Giuliani, A. L., Spisani, S., Cavalletti, T., Reali, E., Melchiorri, L., Ferrari, L., ... Traniello, S. (1993). Fibroblasts increase adhesion to neutrophils after stimulation with phorbol ester and cytokines. Cellular Immunology, 149(1), 208-222. https://doi.org/10.1006/cimm.1993.1148

Fibroblasts increase adhesion to neutrophils after stimulation with phorbol ester and cytokines. / Giuliani, A. L.; Spisani, S.; Cavalletti, T.; Reali, E.; Melchiorri, L.; Ferrari, L.; Lanza, F.; Traniello, S.

In: Cellular Immunology, Vol. 149, No. 1, 1993, p. 208-222.

Research output: Contribution to journalArticle

Giuliani, AL, Spisani, S, Cavalletti, T, Reali, E, Melchiorri, L, Ferrari, L, Lanza, F & Traniello, S 1993, 'Fibroblasts increase adhesion to neutrophils after stimulation with phorbol ester and cytokines', Cellular Immunology, vol. 149, no. 1, pp. 208-222. https://doi.org/10.1006/cimm.1993.1148
Giuliani AL, Spisani S, Cavalletti T, Reali E, Melchiorri L, Ferrari L et al. Fibroblasts increase adhesion to neutrophils after stimulation with phorbol ester and cytokines. Cellular Immunology. 1993;149(1):208-222. https://doi.org/10.1006/cimm.1993.1148
Giuliani, A. L. ; Spisani, S. ; Cavalletti, T. ; Reali, E. ; Melchiorri, L. ; Ferrari, L. ; Lanza, F. ; Traniello, S. / Fibroblasts increase adhesion to neutrophils after stimulation with phorbol ester and cytokines. In: Cellular Immunology. 1993 ; Vol. 149, No. 1. pp. 208-222.
@article{f722d90905ed4ca0b31b48352137386f,
title = "Fibroblasts increase adhesion to neutrophils after stimulation with phorbol ester and cytokines",
abstract = "In our research there was spontaneous adhesion between resting fibroblasts and neutrophils in vitro which could be increased by stimulating either the coculture of cells or each cell type separately with various stimulants. Interferon-γ, interleukin-1, and interleukin-6 significantly increased adhesion; however, the highest adhesive response was obtained when cocultures were treated with phorbol myristate acetate (PMA). As PMA-stimulated fibroblasts show the highest adhesion to resting neutrophils, it was suggested that adhesion was primarily due to an effect on fibroblasts. Without Mg2+ PMA did not stimulate fibroblast adhesion, whereas in the absence of Ca2+ the response was only partially reduced. adhesion was independent of both neutrophil integrins and fibroblast ICAM-1, whereas cytokine-stimulated adhesion was blocked by mAbs against ICAM-1; PMA-stimulated adhesion was not affected by mAbs anti-ICAM-1, but was partially inhibited by mAbs anti-β2 integrins. These results suggested the presence of mechanisms able to modulate the adhesive fibroblast-neutrophil interaction in inflammatory and wound healing processes.",
author = "Giuliani, {A. L.} and S. Spisani and T. Cavalletti and E. Reali and L. Melchiorri and L. Ferrari and F. Lanza and S. Traniello",
year = "1993",
doi = "10.1006/cimm.1993.1148",
language = "English",
volume = "149",
pages = "208--222",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Fibroblasts increase adhesion to neutrophils after stimulation with phorbol ester and cytokines

AU - Giuliani, A. L.

AU - Spisani, S.

AU - Cavalletti, T.

AU - Reali, E.

AU - Melchiorri, L.

AU - Ferrari, L.

AU - Lanza, F.

AU - Traniello, S.

PY - 1993

Y1 - 1993

N2 - In our research there was spontaneous adhesion between resting fibroblasts and neutrophils in vitro which could be increased by stimulating either the coculture of cells or each cell type separately with various stimulants. Interferon-γ, interleukin-1, and interleukin-6 significantly increased adhesion; however, the highest adhesive response was obtained when cocultures were treated with phorbol myristate acetate (PMA). As PMA-stimulated fibroblasts show the highest adhesion to resting neutrophils, it was suggested that adhesion was primarily due to an effect on fibroblasts. Without Mg2+ PMA did not stimulate fibroblast adhesion, whereas in the absence of Ca2+ the response was only partially reduced. adhesion was independent of both neutrophil integrins and fibroblast ICAM-1, whereas cytokine-stimulated adhesion was blocked by mAbs against ICAM-1; PMA-stimulated adhesion was not affected by mAbs anti-ICAM-1, but was partially inhibited by mAbs anti-β2 integrins. These results suggested the presence of mechanisms able to modulate the adhesive fibroblast-neutrophil interaction in inflammatory and wound healing processes.

AB - In our research there was spontaneous adhesion between resting fibroblasts and neutrophils in vitro which could be increased by stimulating either the coculture of cells or each cell type separately with various stimulants. Interferon-γ, interleukin-1, and interleukin-6 significantly increased adhesion; however, the highest adhesive response was obtained when cocultures were treated with phorbol myristate acetate (PMA). As PMA-stimulated fibroblasts show the highest adhesion to resting neutrophils, it was suggested that adhesion was primarily due to an effect on fibroblasts. Without Mg2+ PMA did not stimulate fibroblast adhesion, whereas in the absence of Ca2+ the response was only partially reduced. adhesion was independent of both neutrophil integrins and fibroblast ICAM-1, whereas cytokine-stimulated adhesion was blocked by mAbs against ICAM-1; PMA-stimulated adhesion was not affected by mAbs anti-ICAM-1, but was partially inhibited by mAbs anti-β2 integrins. These results suggested the presence of mechanisms able to modulate the adhesive fibroblast-neutrophil interaction in inflammatory and wound healing processes.

UR - http://www.scopus.com/inward/record.url?scp=0027315821&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027315821&partnerID=8YFLogxK

U2 - 10.1006/cimm.1993.1148

DO - 10.1006/cimm.1993.1148

M3 - Article

C2 - 8099851

AN - SCOPUS:0027315821

VL - 149

SP - 208

EP - 222

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -