Fibroin hydrogels for biomedical applications: Preparation, characterization and in vitro cell culture studies

Antonella Motta, C. Migliaresi, F. Faccioni, P. Torricelli, M. Fini, R. Giardino

Research output: Contribution to journalArticlepeer-review

Abstract

Silk fibroin hydrogels prepared either by treating a 2% (w/v) silk fibroin aqeuous solution at 4°C (thermgel) or by adding 30% (v/v) of glycerol (glygel), were characterized by using Environmental Scanning Electron Microscopy (ESEM), Fourier Transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC), Thermogravimetrical Analysis (TGA) and molecular weight determination. The preparation procedure affected morphology and molecular weight of hydrogels, with no or negligible differences being displayed by FT-IR and DSC analyses. While thermgel presented a well uniform porous structure, the morphology of glygel appeared to be non-porous and heterogeneous. Glygel presented lower water content and lower degradation temperatures, associated with the presence of glycerol but likely also to less-organized protein structures. Cytoxicity tests with human osteoblast-like cells indicated that both gels were not cytoxic, while cell cultures pointed out a faster cell proliferation on glygel and a higher cell activation and differentiation on thermgel. These gels could be used as scaffolds able to promote in situ bone regeneration.

Original languageEnglish
Pages (from-to)851-864
Number of pages14
JournalJournal of Biomaterials Science, Polymer Edition
Volume15
Issue number7
DOIs
Publication statusPublished - 2004

Keywords

  • Biocompatibility
  • Cell cultures
  • Fibroin
  • Hydrogels
  • Silk

ASJC Scopus subject areas

  • Biophysics

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