Fibroin hydrogels for biomedical applications: Preparation, characterization and in vitro cell culture studies

Antonella Motta, C. Migliaresi, F. Faccioni, P. Torricelli, M. Fini, R. Giardino

Research output: Contribution to journalArticle

Abstract

Silk fibroin hydrogels prepared either by treating a 2% (w/v) silk fibroin aqeuous solution at 4°C (thermgel) or by adding 30% (v/v) of glycerol (glygel), were characterized by using Environmental Scanning Electron Microscopy (ESEM), Fourier Transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC), Thermogravimetrical Analysis (TGA) and molecular weight determination. The preparation procedure affected morphology and molecular weight of hydrogels, with no or negligible differences being displayed by FT-IR and DSC analyses. While thermgel presented a well uniform porous structure, the morphology of glygel appeared to be non-porous and heterogeneous. Glygel presented lower water content and lower degradation temperatures, associated with the presence of glycerol but likely also to less-organized protein structures. Cytoxicity tests with human osteoblast-like cells indicated that both gels were not cytoxic, while cell cultures pointed out a faster cell proliferation on glygel and a higher cell activation and differentiation on thermgel. These gels could be used as scaffolds able to promote in situ bone regeneration.

Original languageEnglish
Pages (from-to)851-864
Number of pages14
JournalJournal of Biomaterials Science, Polymer Edition
Volume15
Issue number7
DOIs
Publication statusPublished - 2004

Fingerprint

Fibroins
Hydrogels
Silk
Differential Scanning Calorimetry
Fourier Transform Infrared Spectroscopy
Glycerol
Cell culture
Fourier transform infrared spectroscopy
Differential scanning calorimetry
Gels
Cell Culture Techniques
Molecular Weight
Molecular weight
Bone Regeneration
Osteoblasts
Cell proliferation
Scaffolds
Electron Scanning Microscopy
Water content
Cell Differentiation

Keywords

  • Biocompatibility
  • Cell cultures
  • Fibroin
  • Hydrogels
  • Silk

ASJC Scopus subject areas

  • Biophysics

Cite this

Fibroin hydrogels for biomedical applications : Preparation, characterization and in vitro cell culture studies. / Motta, Antonella; Migliaresi, C.; Faccioni, F.; Torricelli, P.; Fini, M.; Giardino, R.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 15, No. 7, 2004, p. 851-864.

Research output: Contribution to journalArticle

@article{5d46194ea14f4066901975c153bb7ade,
title = "Fibroin hydrogels for biomedical applications: Preparation, characterization and in vitro cell culture studies",
abstract = "Silk fibroin hydrogels prepared either by treating a 2{\%} (w/v) silk fibroin aqeuous solution at 4°C (thermgel) or by adding 30{\%} (v/v) of glycerol (glygel), were characterized by using Environmental Scanning Electron Microscopy (ESEM), Fourier Transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC), Thermogravimetrical Analysis (TGA) and molecular weight determination. The preparation procedure affected morphology and molecular weight of hydrogels, with no or negligible differences being displayed by FT-IR and DSC analyses. While thermgel presented a well uniform porous structure, the morphology of glygel appeared to be non-porous and heterogeneous. Glygel presented lower water content and lower degradation temperatures, associated with the presence of glycerol but likely also to less-organized protein structures. Cytoxicity tests with human osteoblast-like cells indicated that both gels were not cytoxic, while cell cultures pointed out a faster cell proliferation on glygel and a higher cell activation and differentiation on thermgel. These gels could be used as scaffolds able to promote in situ bone regeneration.",
keywords = "Biocompatibility, Cell cultures, Fibroin, Hydrogels, Silk",
author = "Antonella Motta and C. Migliaresi and F. Faccioni and P. Torricelli and M. Fini and R. Giardino",
year = "2004",
doi = "10.1163/1568562041271075",
language = "English",
volume = "15",
pages = "851--864",
journal = "Journal of Biomaterials Science, Polymer Edition",
issn = "0920-5063",
publisher = "Taylor and Francis Ltd.",
number = "7",

}

TY - JOUR

T1 - Fibroin hydrogels for biomedical applications

T2 - Preparation, characterization and in vitro cell culture studies

AU - Motta, Antonella

AU - Migliaresi, C.

AU - Faccioni, F.

AU - Torricelli, P.

AU - Fini, M.

AU - Giardino, R.

PY - 2004

Y1 - 2004

N2 - Silk fibroin hydrogels prepared either by treating a 2% (w/v) silk fibroin aqeuous solution at 4°C (thermgel) or by adding 30% (v/v) of glycerol (glygel), were characterized by using Environmental Scanning Electron Microscopy (ESEM), Fourier Transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC), Thermogravimetrical Analysis (TGA) and molecular weight determination. The preparation procedure affected morphology and molecular weight of hydrogels, with no or negligible differences being displayed by FT-IR and DSC analyses. While thermgel presented a well uniform porous structure, the morphology of glygel appeared to be non-porous and heterogeneous. Glygel presented lower water content and lower degradation temperatures, associated with the presence of glycerol but likely also to less-organized protein structures. Cytoxicity tests with human osteoblast-like cells indicated that both gels were not cytoxic, while cell cultures pointed out a faster cell proliferation on glygel and a higher cell activation and differentiation on thermgel. These gels could be used as scaffolds able to promote in situ bone regeneration.

AB - Silk fibroin hydrogels prepared either by treating a 2% (w/v) silk fibroin aqeuous solution at 4°C (thermgel) or by adding 30% (v/v) of glycerol (glygel), were characterized by using Environmental Scanning Electron Microscopy (ESEM), Fourier Transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC), Thermogravimetrical Analysis (TGA) and molecular weight determination. The preparation procedure affected morphology and molecular weight of hydrogels, with no or negligible differences being displayed by FT-IR and DSC analyses. While thermgel presented a well uniform porous structure, the morphology of glygel appeared to be non-porous and heterogeneous. Glygel presented lower water content and lower degradation temperatures, associated with the presence of glycerol but likely also to less-organized protein structures. Cytoxicity tests with human osteoblast-like cells indicated that both gels were not cytoxic, while cell cultures pointed out a faster cell proliferation on glygel and a higher cell activation and differentiation on thermgel. These gels could be used as scaffolds able to promote in situ bone regeneration.

KW - Biocompatibility

KW - Cell cultures

KW - Fibroin

KW - Hydrogels

KW - Silk

UR - http://www.scopus.com/inward/record.url?scp=4043074966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4043074966&partnerID=8YFLogxK

U2 - 10.1163/1568562041271075

DO - 10.1163/1568562041271075

M3 - Article

C2 - 15318796

AN - SCOPUS:4043074966

VL - 15

SP - 851

EP - 864

JO - Journal of Biomaterials Science, Polymer Edition

JF - Journal of Biomaterials Science, Polymer Edition

SN - 0920-5063

IS - 7

ER -